Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth.

We have isolated and sequenced genes from Saccharomyces cerevisiae (SRP54SC) and Schizosaccharomyces pombe (SRP54sp) encoding proteins homologous to both the 54-kD protein subunit (SRP54mam) of the mammalian signal recognition particle (SRP) and the product of a gene of unknown function in Escherichia coli, ffh (Römisch, K., J. Webb, J. Herz, S. Prehn, R. Frank, M. Vingron, and B. Dobberstein. 1989. Nature (Lond.). 340:478-482; Bernstein H. D., M. A. Poritz, K. Strub, P. J. Hoben, S. Brenner, P. Walter. 1989. Nature (Lond.). 340:482-486). To accomplish this we took advantage of short stretches of conserved sequence between ffh and SRP54mam and used the polymerase chain reaction (PCR) to amplify fragments of the homologous yeast genes. The DNA sequences predict proteins for SRP54sc and SRP54sp that are 47% and 52% identical to SRP54mam, respectively. Like SRP54mam and ffh, both predicted yeast proteins contain a GTP binding consensus sequence in their NH2-terminal half (G-domain), and methionine-rich sequences in their COOH-terminal half (M-domain). In contrast to SRP54mam and ffh the yeast proteins contain additional Met-rich sequences inserted at the COOH-terminal portion of the M-domain. SRP54sp contains a 480-nucleotide intron located 78 nucleotides from the 5' end of the open reading frame. Although the function of the yeast homologues is unknown, gene disruption experiments in S. cerevisiae show that the gene is essential for growth. The identification of SRP54sc and SRP54sp provides the first evidence for SRP related proteins in yeast

Systematic errors and combination of individual CRF solutions in the framework of the international pilot project for the next ICRF

A new international Pilot Project for the re-determination of the ICRF was initiated by the International VLBI Service for Geodesy and Astrometry (IVS) in January 2005. The purpose of this project is to compare the individual CRF solutions and to analyze their systematic and random errors with focus on the selection of the optimal strategy for the next ICRF realization. Eight radio source catalogues provided by the IVS Analysis Centers GA, SHAO, DGFI, GIUB-BKG, JPL, MAO NANU, GSFC, USNO were analyzed. In present study, four analytical models were used to investigate the systematic differences between solutions: solid rotation, rotation and deformation (IERS method), and expansion in orthogonal functions: Legendre-Fourier polynomials and spherical functions. It was found that expansions by orthogonal function describe the differences between individual catalogues better than the two former models. Finally, the combined CRF was generated. Using the radio source positions from this combined catalogue for estimation of EOP has shown improvement of the uncertainty of the celestial pole offset time series.Comment: 9 pages, 8 figures. Presented at the XXVIth IAU General Assembly, JD16, Prague, Czech Republic, 14-25 August 200

Flavor distributions in the nucleons: SU(2) sea asymmetry or isospin symmetry breaking?

The Gottfried sum-rule violation reported by the New Muon Collaboration was interpreted as an indication for a flavor asymmetry of the sea quark in the nucleon. We investigate the alternative possibility that isospin symmetry between the proton and the neutron is breaking. We examine systematically the consequences of this possibility for several processes, namely, neutrino deep inelastic scattering, the charged pion Drell-Yan process, the proton Drell-Yan process, and semi-inclusive deep inelastic scattering, and conclude that a decision between the two alternative explanations is possible

Introduction to the special issue on Ophthalmic Genetics: Vision in 2020

In this special issue of the American Journal of Medical Genetics, Part C, we explore the ever‐expanding field of Ophthalmic Genetics. The eye is unique among organs for its accessibility to physical examination, permitting exploration of every tissue by slit lamp microscopy, ophthalmoscopy, and imaging including color and autofluorescent photography, ultrasound, optical coherence tomography (OCT), electrophysiology, and adaptive optics confocal and scanning laser ophthalmoscopy. This accessibility permits a variety of surgical and nonsurgical treatments, including the first FDA‐approved gene therapy, voretigene neparvovec‐rzyl for RPE65‐associated Leber Congenital Amaurosis. In this issue, we sought to provide a survey highlighting how heritable ophthalmic disorders are recognizable and accessible to clinical geneticists as well as ophthalmologists

Secular Aberration Drift and IAU Definition of ICRS

The gravitational attraction of the Galactic centre leads to the centrifugal acceleration of the Solar system barycentre. It results in secular aberration drift which displaces the position of the distant radio sources. The effect should be accounted for in high-precision astrometric reductions as well as by the corresponding update of the ICRS definition.Comment: 6 page

UV Filters with Antagonistic Action at Androgen Receptors in the MDA-kb2 Cell Transcriptional-Activation Assay

The fact that certain ultraviolet (UV) filters used in cosmetics display estrogenic activity prompted us to study potential actions on androgen receptors (AR) in the human breast carcinoma cell line MDA-kb2, which expresses functional endogenous AR and glucocorticoid receptors (GR) and is stably transfected with a luciferase reporter plasmid. Dihydrotestosterone (DHT), methyltrienolone (R1881), methyltestosterone, danazol, and androstenedione increased luciferase activity, with EC50 values between 0.11 nM (R1881), 0.14 nM (DHT), and 73.5 nM (androstenedione). DHT-induced luciferase gene expression was inhibited by nonsteroidal antiandrogens, hydroxyflutamide, flutamide, bicalutamide, and vinclozolin. In contrast, the steroidal AR agonist/antagonist cyproterone actetate showed agonistic activity in the absence and presence of DHT, which was not blocked by hydroxyflutamide and thus seems not to be mediated by AR. GR-mediated activation of luciferase by dexamethasone was 100 times less potent than DHT and was not antagonized by hydroxyflutamide. The cell line was used for screening of UV filters, benzophenone-3 (Bp-3), benzophenone-4, 3-benzylidene camphor, 4-methylbenzylidene camphor, butyl-methoxy-dibenzoylmethane, homosalate (HMS), octyl-dimethyl-PABA, and octyl-methoxycinnamate. Two of these, Bp-3 and HMS, antagonized DHT-induced AR activation below cytotoxic concentrations, with IC50 of 5.57 10−6 M (HMS) and 4.98 10−6 M (Bp-3). None of the eight UV filters displayed agonistic activity when tested alone, but high concentrations of Bp-3 induced an increase of luciferase activity in the presence of dexamethasone, which was not blocked by hydroxyflutamide or the estrogen antagonist, ICI 182,780. These data indicate that the UV filters Bp-3 and HMS possess antiandrogenic activity in vitro in addition to estrogenic activit

Ocular genetics in the genomics age

Current genetic screening methods for inherited eye diseases are concentrated on the coding exons of known disease genes (gene panels, clinical exome). These tests have a variable and often limited diagnostic rate depending on the clinical presentation, size of the gene panel and our understanding of the inheritance of the disorder (with examples described in this issue). There are numerous possible explanations for the missing heritability of these cases including undetected variants within the relevant gene (intronic, up/down‐stream and structural variants), variants harbored in genes outside the targeted panel, intergenic variants, variants undetectable by the applied technology, complex/non‐Mendelian inheritance, and nongenetic phenocopies. In this article we further explore and review methods to investigate these sources of missing heritability

Silent messages in negotiations: The role of nonverbal communication in cross-cultural business negotiations

This study specifically explored the perceived importance of the following nonverbal factors in the negotiation process: proxemics (location and negotiation site), physical arrangement (seating and furniture arrangement), and kinesics (eye contact, facial expressions and gestures). The participants are professional business negotiators of different nationalities. The findings show that the negotiators\u27 perception about the three factors and their roles in negotiation are consistent with the nonverbal communication literature