3,897 research outputs found
Small RNA sequencing of sporadic amyotrophic lateral sclerosis cerebrospinal fluid reveals differentially expressed miRNAs related to neural and glial activity
Amyotrophic lateral sclerosis (ALS) is a clinical subtype of motor neurone disease (MND), a fatal neurodegenerative disease involving the loss of both the upper and lower motor neurones from the motor cortex, brainstem, and spinal cord. Identifying specific disease biomarkers would help to not only improve diagnostic delay but also to classify disease subtypes, monitor response to therapeutic drugs and track disease progression. miRNAs are small non-coding RNA responsible for regulating gene expression and ultimately protein expression and have been used as biomarkers for many cancers and neurodegenerative disorders. Investigating the detection of miRNAs in cerebrospinal fluid (CSF), the fluid that bathes the central nervous system (CNS) is a prime target for identifying potential biomarkers for ALS. This is the first study to investigate the expression of miRNAs in the CSF of ALS patients using small RNA sequencing. We detected 11 differentially expressed miRNAs in the CSF of sporadic ALS (sALS) patients related to neural and glial activity. Additionally, miRNAs involved in glucose metabolism and the regulation of oxidative stress were also identified. Detecting the presence of potential CSF derived miRNA biomarkers in sALS could open up a whole new area of knowledge to help gain a better understanding of disease pathophysiology. Additionally, with further investigation, the tracking of CSF miRNA over the disease course could be used to follow the disease progression and monitor the effect of novel therapeutics that could be personalized to an individual disease phenotype
The role of e-participation and open data in evidence-based policy decision making in local government
The relationships between policies, their values and outcomes are often difficult for citizens and policy makers to assess due to the complex nature of the policy lifecycle. With the opening of data by public administrations there is now a greater opportunity for transparency, accountability and evidence-based decision making in the policy making process. In representative democracies, citizens rely on their elected representatives and local administrations to take policy decisions that address societal challenges and add value to their local communities. Citizens now have the opportunity to assess the impact and values of the policies introduced by their elected representatives and hold them accountable by utilising historical open data that is publicly available. Using a qualitative case study in a UK Local Government Authority, this paper examines how e-participation platforms and the use of open data can facilitate more factual, evidence based and transparent policy decision making and evaluation. From a theoretical stance, this paper contributes to the policy lifecycle and e-participation literature. The paper also offers valuable insights to public administrations on how open data can be utilised for evidence-based policy decision making and evaluationThis work evolved in the context of the project Policy Compass (http://policycompass.eu/), a project co-funded by the EC within FP7, Grant agreement no: 612133
EquiFACS: the Equine Facial Action Coding System
Although previous studies of horses have investigated their facial expressions in specific contexts, e.g. pain, until now there has been no methodology available that documents all the possible facial movements of the horse and provides a way to record all potential facial configurations. This is essential for an objective description of horse facial expressions across a range of contexts that reflect different emotional states. Facial Action Coding Systems (FACS) provide a systematic methodology of identifying and coding facial expressions on the basis of underlying facial musculature and muscle movement. FACS are anatomically based and document all possible facial movements rather than a configuration of movements associated with a particular situation. Consequently, FACS can be applied as a tool for a wide range of research questions. We developed FACS for the domestic horse (Equus caballus) through anatomical investigation of the underlying musculature and subsequent analysis of naturally occurring behaviour captured on high quality video. Discrete facial movements were identified and described in terms of the underlying muscle contractions, in correspondence with previous FACS systems. The reliability of others to be able to learn this system (EquiFACS) and consistently code behavioural sequences was high—and this included people with no previous experience of horses. A wide range of facial movements were identified, including many that are also seen in primates and other domestic animals (dogs and cats). EquiFACS provides a method that can now be used to document the facial movements associated with different social contexts and thus to address questions relevant to understanding social cognition and comparative psychology, as well as informing current veterinary and animal welfare practices
Chemotherapy versus supportive care in advanced non-small cell lung cancer: improved survival without detriment to quality of life
BACKGROUND: In 1995 a meta-analysis of randomised trials investigating the value of adding chemotherapy to primary treatment for non-small cell lung cancer (NSCLC) suggested a small survival benefit for cisplatin-based chemotherapy in each of the primary treatment settings. However, the metaanalysis included many small trials and trials with differing eligibility criteria and chemotherapy regimens. METHODS: The aim of the Big Lung Trial was to confirm the survival benefits seen in the meta-analysis and to assess quality of life and cost in the supportive care setting. A total of 725 patients were randomised to receive supportive care alone (n = 361) or supportive care plus cisplatin-based chemotherapy (n = 364). RESULTS: 65% of patients allocated chemotherapy (C) received all three cycles of treatment and a further 27% received one or two cycles. 74% of patients allocated no chemotherapy (NoC) received thoracic radiotherapy compared with 47% of the C group. Patients allocated C had a significantly better survival than those allocated NoC: HR 0.77 (95% CI 0.66 to 0.89, p = 0.0006), median survival 8.0 months for the C group v 5.7 months for the NoC group, a difference of 9 weeks. There were 19 (5%) treatment related deaths in the C group. There was no evidence that any subgroup benefited more or less fromchemotherapy. No significant differences were observed between the two groups in terms of the pre-defined primary and secondary quality of life end points, although large negative effects of chemotherapy were ruled out. The regimens used proved to be cost effective, the extra cost of chemotherapy being offset by longer survival. CONCLUSIONS: The survival benefit seen in this trial was entirely consistent with the NSCLC meta-analysis and subsequent similarly designed large trials. The information on quality of life and cost should enablepatients and their clinicians to make more informed treatment choices
A quasi-diagonal approach to the estimation of Lyapunov spectra for spatio-temporal systems from multivariate time series
We describe methods of estimating the entire Lyapunov spectrum of a spatially
extended system from multivariate time-series observations. Provided that the
coupling in the system is short range, the Jacobian has a banded structure and
can be estimated using spatially localised reconstructions in low embedding
dimensions. This circumvents the ``curse of dimensionality'' that prevents the
accurate reconstruction of high-dimensional dynamics from observed time series.
The technique is illustrated using coupled map lattices as prototype models for
spatio-temporal chaos and is found to work even when the coupling is not
strictly local but only exponentially decaying.Comment: 13 pages, LaTeX (RevTeX), 13 Postscript figs, to be submitted to
Phys.Rev.
Synchronisation in Coupled Sine Circle Maps
We study the spatially synchronized and temporally periodic solutions of a
1-d lattice of coupled sine circle maps. We carry out an analytic stability
analysis of this spatially synchronized and temporally periodic case and obtain
the stability matrix in a neat block diagonal form. We find spatially
synchronized behaviour over a substantial range of parameter space. We have
also extended the analysis to higher spatial periods with similar results.
Numerical simulations for various temporal periods of the synchronized
solution, reveal that the entire structure of the Arnold tongues and the
devil's staircase seen in the case of the single circle map can also be
observed for the synchronized coupled sine circle map lattice. Our formalism
should be useful in the study of spatially periodic behaviour in other coupled
map lattices.Comment: uuencoded, 1 rextex file 14 pages, 3 postscript figure
Comparing Galaxy Morphology at Ultraviolet and Optical Wavelengths
We have undertaken an imaging survey of 34 nearby galaxies in far-ultraviolet
(FUV, ~1500A) and optical (UBVRI) passbands to characterize galaxy morphology
as a function of wavelength. This sample, which includes a range of classical
Hubble types from elliptical to irregular with emphasis on spirals at low
inclination angle, provides a valuable database for comparison with images of
high-z galaxies whose FUV light is redshifted into the optical and near-
infrared bands. Ultraviolet data are from the UIT Astro-2 mission. We present
images and surface brightness profiles for each galaxy, and we discuss the
wavelength-dependence of morphology for different Hubble types in the context
of understanding high-z objects. In general, the dominance of young stars in
the FUV produces the patchy appearance of a morphological type later than that
inferred from optical images. Prominent rings and circumnuclear star formation
regions are clearly evident in FUV images of spirals, while bulges, bars, and
old, red stellar disks are faint to invisible at these short wavelengths.
However, the magnitude of the change in apparent morphology ranges from
dramatic in early--type spirals with prominent optical bulges to slight in
late-type spirals and irregulars, in which young stars dominate both the UV and
optical emission. Starburst galaxies with centrally concentrated, symmetric
bursts display an apparent ``E/S0'' structure in the FUV, while starbursts
associated with rings or mergers produce a peculiar morphology. We briefly
discuss the inadequacy of the optically-defined Hubble sequence to describe FUV
galaxy images and estimate morphological k-corrections, and we suggest some
directions for future research with this dataset.Comment: Accepted for publication in the ApJS. 15 pages, 17 JPEG figures, 10
GIF figures. Paper and full resolution figures available at
http://nedwww.ipac.caltech.edu/level5/Kuchinski/frames.htm
Density anomaly in a competing interactions lattice gas model
We study a very simple model of a short-range attraction and an outer shell
repulsion as a test system for demixing phase transition and density anomaly.
The phase-diagram is obtained by applying mean field analysis and Monte Carlo
simulations to a two dimensional lattice gas with nearest-neighbors attraction
and next-nearest-neighbors repulsion (the outer shell). Two liquid phases and
density anomaly are found.
The coexistence line between these two liquid phases meets a critical line
between the fluid and the low density liquid at a tricritical point. The line
of maximum density emerges in the vicinity of the tricritical point, close to
the demixing transition
Ultraviolet Signposts of Resonant Dynamics in the Starburst-Ringed Sab Galaxy, M94 (NGC 4736)
M94 (NGC 4736) is investigated using images from the Ultraviolet Imaging
Telescope (FUV-band), Hubble Space Telescope (NUV-band), Kitt Peak 0.9-m
telescope (H-alpha, R, and I bands), and Palomar 5-m telescope (B-band), along
with spectra from the International Ultraviolet Explorer and Lick 1-m
telescopes. The wide-field UIT image shows FUV emission from (a) an elongated
nucleus, (b) a diffuse inner disk, where H-alpha is observed in absorption, (c)
a bright inner ring of H II regions at the perimeter of the inner disk (R = 48
arcsec. = 1.1 kpc), and (d) two 500-pc size knots of hot stars exterior to the
ring on diametrically opposite sides of the nucleus (R= 130 arcsec. = 2.9 kpc).
The HST/FOC image resolves the NUV emission from the nuclear region into a
bright core and a faint 20 arcsec. long ``mini-bar'' at a position angle of 30
deg. Optical and IUE spectroscopy of the nucleus and diffuse inner disk
indicates an approximately 10^7 or 10^8 yr-old stellar population from
low-level starbirth activity blended with some LINER activity. Analysis of the
H-alpha, FUV, NUV, B, R, and I-band emission along with other observed tracers
of stars and gas in M94 indicates that most of the star formation is being
orchestrated via ring-bar dynamics involving the nuclear mini-bar, inner ring,
oval disk, and outer ring. The inner starburst ring and bi-symmetric knots at
intermediate radius, in particular, argue for bar-mediated resonances as the
primary drivers of evolution in M94 at the present epoch. Similar processes may
be governing the evolution of the ``core-dominated'' galaxies that have been
observed at high redshift. The gravitationally-lensed ``Pretzel Galaxy''
(0024+1654) at a redshift of approximately 1.5 provides an important precedent
in this regard.Comment: revised figure 1 (corrected coordinate labels on declination axis);
19 pages of text + 19 figures (jpg files); accepted for publication in A
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