PyPHLAWD: A python tool for phylogenetic dataset construction

Comprehensive phylogenetic trees are essential for many ecological and evolutionary studies. Researchers may use existing trees or construct their own. In order to infer new trees, researchers often rely on programmes that construct datasets from publicly available molecular data.Here, we present PyPHLAWD, a phylogenetically guided tool written in python that creates molecular datasets for building trees. PyPHLAWD constructs clusters (putative orthologs) that may be used for downstream analyses and provides users with a set of easy to interpret results. PyPHLAWD can conduct both baited (analyses that require the identification of gene regions a priori) and clustering analyses (analyses that do not require a priori identification of gene regions).PyPHLAWD is extensible, flexible, open source, and available at https://github.com/FePhyFoFum/PyPHLAWD, with a detailed website outlining instructions and functionality at https://fephyfofum.github.io/PyPHLAWD/.The utility of PyPHLAWD is highlighted here with an example workflow for the plant clade Dipsacales and may be applied to any clade with publicly available data on GenBank.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147863/1/mee313096_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147863/2/mee313096.pd

Flight Determination of the Static Longitudinal Stability Boundaries of the Bell X-5 Research Airplane with 59 Deg Sweepback

During the flight program on the Bell X-5 airplane with 59 deg sweepback to determine the practical Mach number and normal-force coefficient limits of this configuration, a reduction in static longitudinal stability was encountered in maneuvering flight. A determination of the boundary for reduction of longitudinal stability extending to a Mach number of 0.98 is presented in this paper. A reduction of static longitudinal stability existed for all elevator and all stabilizer-executed maneuvers. The reduction of stability existed for maneuvers executed with elevator near a normal-force coefficient of 0.6 for a Mach number range of about 0.31 to 0.76. Above a Mach number of 0.76 the normal-force coefficient for reduction of stability gradually decreased to a value of 0.2 at a Mach number of 0.98. For stabilizer-executed maneuvers the stability boundary was the same as for elevator maneuvers up to a Mach number of 0.88. Above this Mach number the reduction of stability occurred at slightly higher normal-force coefficients decreasing from about 0.51 at a Mach number of 0.92 to a value of 0.311 at a Mach number of 0.97. The airplane has been flown to a Mach number of 1.04 at a normal-force coefficient of about 0.15 without encountering any reduction of stability. The pilot did not consider the reduction of stability to be dangerous at altitudes above 30,000 feet; however, precise flight was impossible. At angles of attack above that at which the reduction of longitudinal stability occurred, directional instability and aileron control overbalance were encountered

Archives of the Congregation of the Mission, Province of the West

This inventory of the Province of the West’s archives reflects its holdings at the end of 1988, with some notable additions. Highlights include materials on the Congregation’s government, including minutes, working papers, and other documents for general assemblies; records for each American province (with the greatest emphasis on the Province of the West), reflecting the Congregation’s historical growth in the United States; published works on the Daughters of Charity; various items on the Ladies of Charity and the Saint Vincent de Paul Society; publications (amounting to hundreds of titles) on Vincent de Paul, Louise de Marillac, Catherine Laboure, and Elizabeth Ann Seton; and various audio-visual materials

Individual-specific changes in the human gut microbiota after challenge with enterotoxigenic Escherichia coli and subsequent ciprofloxacin treatment

Acknowledgements The authors wish to thank Mark Stares, Richard Rance, and other members of the Wellcome Trust Sanger Institute’s 454 sequencing team for generating the 16S rRNA gene data. Lili Fox Vélez provided editorial support. Funding IA, JNP, and MP were partly supported by the NIH, grants R01-AI-100947 to MP, and R21-GM-107683 to Matthias Chung, subcontract to MP. JNP was partly supported by an NSF graduate fellowship number DGE750616. IA, JNP, BRL, OCS and MP were supported in part by the Bill and Melinda Gates Foundation, award number 42917 to OCS. JP and AWW received core funding support from The Wellcome Trust (grant number 098051). AWW, and the Rowett Institute of Nutrition and Health, University of Aberdeen, receive core funding support from the Scottish Government Rural and Environmental Science and Analysis Service (RESAS).Peer reviewedPublisher PD