BENEFIT-COST ANALYSIS OF INCOME MAINTENANCE PROPOSALS

Public Economics,

Infectious abortion in swine

Cover title.Includes bibliographical references

Thinking About Privacy: Chapter 1 of "Engaging Privacy and Information Technology in a Digital Age"

Just as recent centuries saw transitions from the agricultural to the industrial to the information age and associated societal and technological changes, the early 21st century will continue to pose dynamic challenges in many aspects of society. Most importantly from the standpoint of this report, advances in information technology are proceeding apace. In this rapidly changing technological context, individuals, institutions, and governments will be forced to reexamine core values, beliefs, laws, and social structures if their understandings of autonomy, privacy, justice, community, and democracy are to continue to have meaning. A central concept throughout U.S. history has been the notion of privacy and the creation of appropriate borders between the individual and the state. In the latter 19th century, as industrial urban society saw the rise of large bureaucratic organizations, notions of privacy were extended to the borders between private organizations and the individual. This report focuses on privacy and its intersections with information technology and associated social and technology trends

A high-throughput immobilized bead screen for stable proteins and multi-protein complexes

We describe an in vitro colony screen to identify Escherichia coli expressing soluble proteins and stable, assembled multiprotein complexes. Proteins with an N-terminal 6His tag and C-terminal green fluorescent protein (GFP) S11 tag are fluorescently labeled in cells by complementation with a coexpressed GFP 1–10 fragment. After partial colony lysis, the fluorescent soluble proteins or complexes diffuse through a supporting filtration membrane and are captured on Talon® resin metal affinity beads immobilized in agarose. Images of the fluorescent colonies convey total expression and the level of fluorescence bound to the beads indicates how much protein is soluble. Both pieces of information can be used together when selecting clones. After the assay, colonies can be picked and propagated, eliminating the need to make replica plates. We used the method to screen a DNA fragment library of the human protein p85 and preferentially obtained clones expressing the full-length ‘breakpoint cluster region-homology' and NSH2 domains. The assay also distinguished clones expressing stable multi-protein complexes from those that are unstable due to missing subunits. Clones expressing stable, intact heterotrimeric E.coli YheNML complexes were readily identified in libraries dominated by complexes of YheML missing the N subunit

The improvement of renal survival with steroid pulse therapy in IgA nephropathy

Background. The benefits of steroid therapy in immunoglobulin A nephropathy (IgAN) have not been established

PH207x: Health in Numbers and PH278x: Human Health and Global Environmental Change: 2012-2013 Course Report

In the 2012-2013 academic year, the first two Harvard School of Public Health courses were offered through HarvardX on the edX platform: PH207x: Health in Numbers and PH278x: Human Health and Global Environmental Change. They were taught by Professors Earl Francis Cook and Marcello Pagano, and Aaron Bernstein and Jack Spengler, respectively. This report describes the structure of these two courses, the demographic characteristics of registrants, and the activity of students. This report was prepared by researchers external to the course teams and is based on examination of the courseware, analyses of the data collected by the edX platform, and interviews and consultations with the course faculty and team members

HarvardX and MITx: The First Year of Open Online Courses, Fall 2012-Summer 2013

HarvardX and MITx are collaborative institutional efforts between Harvard University and MIT to enhance campus-based education, advance educational research, and increase access to online learning opportunities worldwide. Over the year from the fall of 2012 to the summer of 2013, HarvardX and MITx launched 17 courses on edX, a jointly founded platform for delivering massive open online courses (MOOCs). In that year, 43,196 registrants earned certificates of completion. Another 35,937 registrants explored half or more of course content without certification. An additional 469,702 registrants viewed less than half of the content. And 292,852 registrants never engaged with the online content. In total, there were 841,687 registrations from 597,692 unique users across the first year of HarvardX and MITx courses. This report is a joint effort by institutional units at Harvard and MIT to describe the registrant and course data provided by edX in the context of the diverse efforts and intentions of HarvardX and MITx instructor teams

Engaging Privacy and Information Technology in a Digital Age: Executive Summary

James Waldo Helen Nissenbaum Sun Microsystems, New York University Vice Chair Robert M. O'Neil Julie E. Cohen University of Virginia Georgetown University Janey Place Robert W. Crandall Digital Thinking Brookings Institution (resigned April 2006) Ronald L. Rivest Oscar Gandy, Jr. Massachusetts Institute of Technology University of Pennsylvania Teresa Schwartz James Horning George Washington University Network Associates Laboratories Lloyd N. Cutler Gary King Wilmer, Cutler, Pickering, Hale & Dorr LLP, Harvard University served as co-chair until his passing in May 2005. Lin E. Knapp, Independent Consultant Ponte Vedra Beach, Florid

The optimization of in vitro high-throughput chemical lysis of Escherichia coli. Application to ACP domain of the polyketide synthase ppsC from Mycobacterium tuberculosis

Protein production in Escherichia coli involves high-level expression in a culture, followed by harvesting of the cells and finally their disruption, or lysis, to release the expressed proteins. We compare three high-throughput chemical lysis methods to sonication, using a robotic platform and methodologies developed in our laboratory [1]. Under the same expression conditions, all lysis methods varied in the degree of released soluble proteins. With a set of 96 test proteins, we used our split GFP to quantify the soluble and insoluble protein fractions after lysis. Both the amount of soluble protein and the percentage recovered in the soluble fraction using SoluLyse® were well correlated with sonication. Two other methods, Bugbuster® and lysozyme, did not correlate well with sonication. Considering the effects of lysis methods on protein solubility is especially important when accurate protein solubility measurements are needed, for example, when testing adjuvants, growth media, temperature, or when establishing the effects of truncation or sequence variation on protein stability

ER22x: JusticeX: Spring 2013 Course Report

ER22x was offered as a HarvardX course in Spring 2013 on edX, a platform for massive open online courses (MOOCs). It was taught by Professor Michael Sandel. The report was prepared by researchers external to the course team, based on an examination of the courseware, analyses of data collected by the edX platform, and interviews with the course faculty and team members