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Vinculin binding angle in podosomes revealed by high resolution microscopy
Podosomes are highly dynamic actin-rich adhesive structures formed predominantly by cells of the monocytic lineage, which degrade the extracellular matrix. They consist of a core of F-actin and actin-regulating proteins, surrounded by a ring of adhesion-associated proteins such as vinculin. We have characterised the structure of podosomes in macrophages, particularly the structure of the ring, using three super-resolution fluorescence microscopy techniques: stimulated emission depletion microscopy, structured illumination microscopy and localisation microscopy. Rather than being round, as previously assumed, we found the vinculin ring to be created from relatively straight strands of vinculin, resulting in a distinctly polygonal shape. The strands bind preferentially at angles between 116° and 135°. Furthermore, adjacent vinculin strands are observed nucleating at the corners of the podosomes, suggesting a mechanism for podosome growth
Chasing Hypernyms in Vector Spaces with Entropy
In this paper, we introduce SLQS, a new entropy-based measure for the unsupervised identification of hypernymy and its directionality in Distributional Semantic Models (DSMs). SLQS is assessed through two tasks: (i.) identifying the hypernym in hyponym-hypernym pairs, and (ii.) discriminating hypernymy among various semantic relations. In both tasks, SLQS outperforms other state-of-the-art measures
Surviving historical Patagonian landscapes and climate: molecular insights from Galaxias maculatus
<p>Abstract</p> <p>Background</p> <p>The dynamic geological and climatic histories of temperate South America have played important roles in shaping the contemporary distributions and genetic diversity of endemic freshwater species. We use mitochondria and nuclear sequence variation to investigate the consequences of mountain barriers and Quaternary glacial cycles for patterns of genetic diversity in the diadromous fish <it>Galaxias maculatus </it>in Patagonia (~300 individuals from 36 locations).</p> <p>Results</p> <p>Contemporary populations of <it>G. maculatus</it>, east and west of the Andes in Patagonia, represent a single monophyletic lineage comprising several well supported groups. Mantel tests using control region data revealed a strong positive relationship when geographic distance was modeled according to a scenario of marine dispersal. (<it>r </it>= 0.69, <it>P = 0.055</it>). By contrast, direct distance between regions was poorly correlated with genetic distance (<it>r </it>= -0.05, <it>P </it>= 0.463). Hierarchical AMOVAs using mtDNA revealed that pooling samples according to historical (pre-LGM) oceanic drainage (Pacific vs. Atlantic) explained approximately four times more variance than pooling them into present-day drainage (15.6% vs. 3.7%). Further <it>post-hoc </it>AMOVA tests revealed additional genetic structure between populations east and west of the Chilean Coastal Cordillera (coastal vs. interior). Overall female effective population size appears to have remained relatively constant until roughly 0.5 Ma when population size rapidly increased several orders of magnitude [100× (60×-190×)] to reach contemporary levels. Maximum likelihood analysis of nuclear alleles revealed a poorly supported gene tree which was paraphyletic with respect to mitochondrial-defined haplogroups.</p> <p>Conclusions</p> <p>First diversifying in the central/north-west region of Patagonia, <it>G. maculatus </it>extended its range into Argentina via the southern coastal regions that join the Pacific and Atlantic oceans. More recent gene flow between northern populations involved the most ancient and most derived lineages, and was likely facilitated by drainage reversal(s) during one or more cooling events of the late Pleistocene. Overall female effective population size represents the end result of a widespread and several hundred-fold increase over approximately 0.5 Ma, spanning several climatic fluctuations of the Pleistocene. The minor influence of glacial cycles on the genetic structure and diversity of <it>G. maculatus </it>likely reflects the access to marine refugia during repeated bouts of global cooling. Evidence of genetic structure that was detected on a finer scale between lakes/rivers is most likely the result of both biological attributes (i.e., resident non-migratory behavior and/or landlocking and natal homing in diadromous populations), and the Coastal Cordillera as a dispersal barrier.</p
Non-Compositional Term Dependence for Information Retrieval
Modelling term dependence in IR aims to identify co-occurring terms that are
too heavily dependent on each other to be treated as a bag of words, and to
adapt the indexing and ranking accordingly. Dependent terms are predominantly
identified using lexical frequency statistics, assuming that (a) if terms
co-occur often enough in some corpus, they are semantically dependent; (b) the
more often they co-occur, the more semantically dependent they are. This
assumption is not always correct: the frequency of co-occurring terms can be
separate from the strength of their semantic dependence. E.g. "red tape" might
be overall less frequent than "tape measure" in some corpus, but this does not
mean that "red"+"tape" are less dependent than "tape"+"measure". This is
especially the case for non-compositional phrases, i.e. phrases whose meaning
cannot be composed from the individual meanings of their terms (such as the
phrase "red tape" meaning bureaucracy). Motivated by this lack of distinction
between the frequency and strength of term dependence in IR, we present a
principled approach for handling term dependence in queries, using both lexical
frequency and semantic evidence. We focus on non-compositional phrases,
extending a recent unsupervised model for their detection [21] to IR. Our
approach, integrated into ranking using Markov Random Fields [31], yields
effectiveness gains over competitive TREC baselines, showing that there is
still room for improvement in the very well-studied area of term dependence in
IR
Characterizing response types and revealing noun ambiguity in German association norms
This paper presents an analysis of semantic association norms for German nouns. In contrast to prior studies, we not only collected associations elicited by written representations of target objects but also by their pictorial representations. In a first analysis, we identified systematic differences in the type and distribution of associate responses for the two presentation forms. In a second analysis, we applied a soft cluster analysis to the collected target-response pairs. We subsequently used the clustering to predict noun ambiguity and to discriminate senses in our target nouns
Nanopatterned sapphire substrates in deep UV LEDs is there an optical benefit?
Light emitting diodes LEDs in the deep ultra violet DUV offer new perspectives for multiple applications ranging from 3D printing to sterilization. However, insufficient light extraction severely limits their efficiency. Nanostructured sapphire substrates in aluminum nitride based LED devices have recently shown to improve crystal growth properties, while their impact on light extraction has not been fully verified. We present a model for understanding the impact of nanostructures on the light extraction capability of DUV LEDs. The model assumes an isotropic light source in the semiconductor layer stack and combines rigorously computed scattering matrices with a multilayer solver. We find that the optical benefit of using a nanopatterned as opposed to a planar sapphire substrate to be negligible, if parasitic absorption in the p side of the LED is dominant. If losses in the p side are reduced to 20 , then for a wavelength of 265 ,nm an increase of light extraction efficiency from 7.8 to 25.0 is possible due to nanostructuring. We introduce a concept using a diffuse Lambertian reflector as p contact, further increasing the light extraction efficiency to 34.2 . The results underline that transparent p sides and reflective p contacts in DUV LEDs are indispensable for enhanced light extraction regardless of the interface texture between semiconductor and sapphire substrate. The optical design guidelines presented in this study will accelerate the development of high efficiency DUV LEDs. The model can be extended to other multilayer opto electronic nanostructured devices such as photovoltaics or photodetector
Electrostatically gated membrane permeability in inorganic protocells
Although several strategies are now available to produce functional microcompartments analogous to primitive cell-like structures, little progress has been made in generating protocell constructs with self-controlled membrane permeability. Here we describe the preparation of water-dispersible colloidosomes based on silica nanoparticles and delineated by a continuous semipermeable inorganic membrane capable of self-activated, electrostatically gated permeability. We use crosslinking and covalent grafting of a pH-responsive copolymer to generate an ultrathin elastic membrane that exhibits selective release and uptake of small molecules. This behaviour, which depends on the charge of the copolymer coronal layer, serves to trigger enzymatic dephosphorylation reactions specifically within the protocell aqueous interior. This system represents a step towards the design and construction of alternative types of artificial chemical cells and protocell models based on spontaneous processes of inorganic self-organization
Closed reduction and percutaneus Kirschner wire fixation for the treatment of dislocated calcaneal fractures: surgical technique, complications, clinical and radiological results after 2–10 years
AlN overgrowth of nano-pillar-patterned sapphire with different offcut angle by metalorganic vapor phase epitaxy
We present overgrowth of nano-patterned sapphire with different offcut angles by metalorganic vapor phase epitaxy. Hexagonal arrays of nano-pillars were prepared via Displacement Talbot Lithography and dry-etching. 6.6 µm crack-free and fully coalesced AlN was grown on such substrates. Extended defect analysis comparing X-ray diffraction, electron channeling contrast imaging and selective defect etching revealed a threading dislocation density of about 109 cm−2. However, for c-plane sapphire offcut of 0.2° towards m direction the AlN surface shows step bunches with a height of 10 nm. The detrimental impact of these step bunches on subsequently grown AlGaN multi-quantum-wells is investigated by cathodoluminescence and transmission electron microscopy. By reducing the sapphire offcut to 0.1° the formation of step bunches is successfully suppressed. On top of such a sample an AlGaN-based UVC LED heterostructure is realized emitting at 265 nm and showing an emission power of 0.81 mW at 20 mA (corresponds to an external quantum efficiency of 0.86%)
AlN overgrowth of nano-pillar-patterned sapphire with different offcut angle by metalorganic vapor phase epitaxy
We present overgrowth of nano-patterned sapphire with different offcut angles by metalorganic vapor phase epitaxy. Hexagonal arrays of nano-pillars were prepared via Displacement Talbot Lithography and dry-etching. 6.6 µm crack-free and fully coalesced AlN was grown on such substrates. Extended defect analysis comparing X-ray diffraction, electron channeling contrast imaging and selective defect etching revealed a threading dislocation density of about 109 cm-2. However, for c-plane sapphire offcut of 0.2° towards m direction the AlN surface shows step bunches with a height of 10 nm. The detrimental impact of these step bunches on subsequently grown AlGaN multi-quantum-wells is investigated by cathodoluminescence and transmission electron microscopy. By reducing the sapphire offcut to 0.1° the formation of step bunches is successfully suppressed. On top of such a sample an AlGaN-based UVC LED heterostructure is realized emitting at 265 nm and showing an emission power of 0.81 mW at 20 mA (corresponds to an external quantum efficiency of 0.86 %)
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