760 research outputs found
Pseudospectra and stability radii of analytic matrix functions with application to time-delay systems
AbstractDefinitions for pseudospectra and stability radii of an analytic matrix function are given, where the structure of the function is exploited. Various perturbation measures are considered and computationally tractable formulae are derived. The results are applied to a class of retarded delay differential equations. Special properties of the pseudospectra of such equations are determined and illustrated
Fluorogenic and bioorthogonal modification of rna using photoclick chemistry
A bromoaryltetrazole-modified uridine was synthesized as a new RNA building block for bioorthogonal, light-activated and postsynthetic modification with commercially available fluorescent dyes. It allows âphotoclickâ-type modifications by irradiation with light (300 nm LED) at internal and terminal positions of presynthesized RNA with maleimide-conjugated fluorophores in good yields. The reaction was evidenced for three different dyes. During irradiation, the emission increases due to the formation of an intrinsically fluorescent pyrazoline moiety as photoclick product. The fluorogenecity of the photoclick reaction was significantly enhanced by energy transfer between the pyrazoline as the reaction product (poor emitter) and the photoclicked dye as the strong emitter. The RNA-dye conjugates show remarkable fluorescent properties, in particular an up to 9.4 fold increase of fluorescence, which are important for chemical biology and fluorescent imaging of RNA in cells
Testing for Antiphospholipid Antibody (aPL) Specificities in Retrospective âNormalâ Cerebral Spinal Fluid (CSF)
Antiphospholipid antibodies (aPL) have been found in the blood of patients with systemic and
neurological disease. The rare reports of aPL in cerebral spinal fluid (CSF) have been limited mostly to
IgG and IgM anticardiolipin (aCL). Our published finding of IgA aPE in the CSF of a young stroke
victim prompted us to establish ânormalâ CSF aPL values for a panel of aPL, which included aCL,
antiphosphatidylserine (aPS), antiphosphatidylethanolamine (aPE) and antiphosphatidylcholine (aPC).
CSF samples were tested by ELISA for IgG, IgM and IgA aPL. In addition, the CSF samples were
tested for activity in the presence and absence of phospholipid (PL) binding plasma-proteins. A total of
24 data points were obtained for each CSF sample.We tested 59 CSF samples obtained from 59 patients
who were undergoing evaluation for systemic or neurologic diseases. All CSF samples had normal
protein, glucose and cell counts. Ten of the 59 CSF samples (17%) had elevated aPL optical density
(OD) values an order of magnitude higher than the other 49 CSF samples for one or more aPL
specificity and/or isotype. One CSF sample had both PL-binding protein dependent and independent
IgG aPE activity. Another CSF sample showed both IgG aPE and aPC reactivity. The remaining eight
CSF samples showed single aPL findings; IgG aPE (5), IgG aPC (1), IgG aCL (1) and IgM aPC (1).
Seven of 10 patients with elevated CSF values were females. As expected, most ânormalâ aPL OD
values were substantially lower in CSF than those we have reported in blood samples from volunteer
blood donors
Quantitative correlation of cardiac glycoside binding to its receptor and inhibition of the sodium pump in chicken heart cells in culture
Fast and Efficient Postsynthetic DNA Labeling in Cells by Means of Strain-Promoted Sydnone-Alkyne Cycloadditions
Fast and efficient: DNA strands, modified with the novel bioorthogonal reporters sydnones, undergo fast and efficient labeling with cyclooctynes and have the potential to become essential tools for imaging DNA and possibly RNA in cells.
Sydnones are highly stable mesoionic 1,3-dipoles that react with cyclooctynes through strain-promoted sydnone-alkyne cycloaddition (SPSAC). Although sydnones have been shown to be valuable bioorthogonal chemical reporters for the labeling of proteins and complex glycans, nucleic acids have not yet been tagged by SPSAC. Evaluation of SPSAC kinetics with model substrates showed fast reactions with cyclooctyne probes (up to k=0.59â
Mâs), and two different sydnones were effectively incorporated into both 2â-deoxyuridines at position 5, and 7-deaza-2â-deoxyadenosines at position 7. These modified nucleosides were synthetically incorporated into single-stranded DNAs, which were successfully postsynthetically labeled with cyclooctyne probes both inâ
vitro and in cells. These results show that sydnones are versatile bioorthogonal tags and have the premise to become essential tools for tracking DNA and potentially RNA in living cells
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