The Side-Effects of the Space Charge Field Introduced by Hollow Electron Beam in the Electron Cooler of CSRm

Electron cooler is used to improve the quality of the beam in synchrotron, however it also introduces nonlinear electromagnetic field, which cause tuneshift, tunespread and may drive resonances leading to beam loss. In this paper the tuneshift and the tunespread caused by nonlinear electromagnetic field of the hollow electron beam was investigated, and the resonance driving terms of the nonlinear electromagnetic field was analysed. The differences were presented comparing with the solid electron beam. The calculations were performed for $^{238}U^{32+}$ ions of energy 1.272MeV stored in CSRm, using the parameters given in table1. The conclusion is that in this situation nonlinear field caused by the hollow electron beam do not lead to serious resonances

Ku80 cooperates with CBP to promote COX-2 expression and tumor growth.

Cyclooxygenase-2 (COX-2) plays an important role in lung cancer development and progression. Using streptavidin-agarose pulldown and proteomics assay, we identified and validated Ku80, a dimer of Ku participating in the repair of broken DNA double strands, as a new binding protein of the COX-2 gene promoter. Overexpression of Ku80 up-regulated COX-2 promoter activation and COX-2 expression in lung cancer cells. Silencing of Ku80 by siRNA down-regulated COX-2 expression and inhibited tumor cell growth in vitro and in a xenograft mouse model. Ku80 knockdown suppressed phosphorylation of ERK, resulting in an inactivation of the MAPK pathway. Moreover, CBP, a transcription co-activator, interacted with and acetylated Ku80 to co-regulate the activation of COX-2 promoter. Overexpression of CBP increased Ku80 acetylation, thereby promoting COX-2 expression and cell growth. Suppression of CBP by a CBP-specific inhibitor or siRNA inhibited COX-2 expression as well as tumor cell growth. Tissue microarray immunohistochemical analysis of lung adenocarcinomas revealed a strong positive correlation between levels of Ku80 and COX-2 and clinicopathologic variables. Overexpression of Ku80 was associated with poor prognosis in patients with lung cancers. We conclude that Ku80 promotes COX-2 expression and tumor growth and is a potential therapeutic target in lung cancer

Effects of incubation temperature, starter culture level and total solids content on the rheological properties of yogurt

The effects of incubation temperature (35-45oC), starter culture level (0.003-0.006%, w/v), and milk total solids content (12.32-15.68%, w/v) on the rheological properties of yogurt were investigated using central composite rotatable design (CCRD) and response surface methodology (RSM). Gelation time during milk fermentation and apparent viscosity of yogurt stored for 3 days were the rheological parameters studied. The results revealed that the gelation time and apparent viscosity of yogurt were significantly affected (P < 0.1) by incubation temperature, starter culture level, and total solids. Incubation temperature had the greatest influence on the gelation time. Higher temperature led to shorter gelation time, but resulted in lower apparent viscosity of yogurt. Apparent viscosity of yogurt was mainly affected by total solids. Higher apparent viscosities were also obtained at lower incubation temperature

Selection of Peptide Inhibitor to Matrix Metalloproteinase-2 Using Phage Display and Its Effects on Pancreatic Cancer Cell lines PANC-1 and CFPAC-1

Despite tremendous advances in cancer treatment and survival rates, pancreatic cancer remains one of the most deadly afflictions and the fourth leading cause of cancer deaths in the world. Matrix Metalloproteinases (MMPs) are thought to be involved in cancer progression. Matrix metalloproteinase (MMP)-2 is known to play a pivotal role in tumor invasion, metastasis and angiogenesis, and validated to be the anticancer target. Inhibition of MMP-2 activity is able to reduce the cancer cell invasion and suppress tumor growth in vivo. Two novel peptides, M204C4 and M205C4, which could specially inhibit MMP-2 activity, were identified by a phage display library screening. We showed that M204C4 and M205C4 inhibited the activity of MMP-2 in a dose dependent manner in vitro. Two peptides reduced MMP-2 mediated invasion of the pancreatic cancer cell lines PANC-1 and CFPAC-1, but not affected the expression and release of MMP-2. Furthermore, these two peptides could suppress tumor growth in vivo. Our results indicated that two peptides selected by phase display technology may be used as anticancer drugs in the future

Learning to Solve Geometry Problems via Simulating Human Dual-Reasoning Process

Geometry Problem Solving (GPS), which is a classic and challenging math problem, has attracted much attention in recent years. It requires a solver to comprehensively understand both text and diagram, master essential geometry knowledge, and appropriately apply it in reasoning. However, existing works follow a paradigm of neural machine translation and only focus on enhancing the capability of encoders, which neglects the essential characteristics of human geometry reasoning. In this paper, inspired by dual-process theory, we propose a Dual-Reasoning Geometry Solver (DualGeoSolver) to simulate the dual-reasoning process of humans for GPS. Specifically, we construct two systems in DualGeoSolver, namely Knowledge System and Inference System. Knowledge System controls an implicit reasoning process, which is responsible for providing diagram information and geometry knowledge according to a step-wise reasoning goal generated by Inference System. Inference System conducts an explicit reasoning process, which specifies the goal in each reasoning step and applies the knowledge to generate program tokens for resolving it. The two systems carry out the above process iteratively, which behaves more in line with human cognition. We conduct extensive experiments on two benchmark datasets, GeoQA and GeoQA+. The results demonstrate the superiority of DualGeoSolver in both solving accuracy and robustness from explicitly modeling human reasoning process and knowledge application.Comment: IJCAI 2024 Accepte

SHEARING BEHAVIOR OF STRUCTURAL INSULATED PANEL WALL SHELLED WITH BAMBOO SCRIMBER

In this study, shearing behavior of a structural insulated panel (SIP) wall, which consisted of a Styrofoam core board, shell panel of bamboo scrimber, and frame of Spruce–Pine–Fir dimension lumber, was tested under monotonic and cyclic loads. Results showed that the SIP wall failed at similar positions under two loading modes, although more serious destruction occurred under cyclic than monotonic load. There was a linear relationship between load and displacement at the initial loading stage, which indicated that the wall worked under the elastic state. At a later loading stage, bearing capacity and rigidity decreased as a result of wall slip. Shearing strength under monotonic and cyclic loads was 20.0 and 15.8 kNm-1, respectively, which met the requirement of the standard code for design of timber structures. Energy consumption of the SIP wall covered with bamboo scrimber was 11,556.6 Jm-1

Data-driven state monitoring of air preheater using density peaks clustering and evidential Knearest neighbour classifier

Data-driven state monitoring requiring a little priori knowledge plays a key role for timely fault detection and is therefore of great importance for the safe and economical operation of the thermal power plant. The main drawback for most of the existing data-driven methods is the complex procedure of data preprocessing and model training especially when unlabelled operating data is used. To overcome this issue, this paper proposes a new framework of data-driven state monitoring approach for the thermal power plant devices. The approach is composed of two steps. In the first step, density peaks clustering(DPC) is performed on the historical data to generate labels for the data. Then in the second step, evidential K-nearest neighbour(EKNN) method is used to monitor the current state based on the labelled historical data and operating data. Verifications on operating data of an air preheater system of a 1000MW thermal power plant show that the proposed method can identify various air leakage states accurately and efficiently

Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic Carcinoma

Objective: Cathepsin D (CTSD) is a pivotal orchestrator in the occurrence and development of tumors. Recently, CTSD was detected in salivary adenoid cystic carcinoma (SACC). However, its functional role in perineural invasion (PNI) of SACC remained elusive. We conducted the present study to detect the expression of CTSD in SACC, analyze the correlation between CTSD expression and prognosis of SACC patients and elucidate the role of CTSD in occurrence of PNI in SACC to lay the foundation for further studies.Methods: Immunohistochemical analysis was conducted to assess CTSD and Ki67 expression in 158 SACC samples and 20 normal salivary gland samples adjacent to carcinoma. Meanwhile, the correlation between CTSD and PNI of SACC specimens was analyzed using Wilcoxon test. QRT-PCR, immunofluorescence and western blot analysis were used to examine the levels of CTSD mRNA and protein in SACC-LM cell line. SiRNA-mediated CTSD silence was performed. Scratch wound healing assay, transwell invasion assay and DRG co-culture assay of PNI was used to detect the ability of migration, invasion and PNI. FITC-phalloidin was used to detect cytoskeletal organization.Results: Our data demonstrated that the positive expression of CTSD was observed in 74.1% (117/158) of SACC cases, and the expression of CTSD was significantly correlated with the PNI (p &lt; 0.05). The ability of migration, invasion, and PNI could be inhibited significantly by siRNA-mediated CTSD silence (p &lt; 0.01). Furthermore, siRNA-mediated CTSD silence inhibited cytoskeletal organization and pseudo foot formation in SACC-LM cells.Conclusion: Our results suggested that an association between PNI and expression of CTSD existed. CTSD may promote PNI of SACC accompanied by cytoskeletal organization and pseudo foot formation

A population of innate myelolymphoblastoid effector cell expanded by inactivation of mTOR complex 1 in mice

Adaptive autoimmunity is restrained by controlling population sizes and pathogenicity of harmful clones, while innate destruction is controlled at effector phase. We report here that deletion of Rptor in mouse hematopoietic stem/progenitor cells causes self-destructive innate immunity by massively increasing the population of previously uncharacterized innate myelolymphoblastoid effector cells (IMLECs). Mouse IMLECs are CD3-B220-NK1.1-Ter119- CD11clow/-CD115-F4/80low/-Gr-1- CD11b+, but surprisingly express high levels of PD-L1. Although they morphologically resemble lymphocytes and actively produce transcripts from Immunoglobulin loci, IMLECs have non-rearranged Ig loci, are phenotypically distinguishable from all known lymphocytes, and have a gene signature that bridges lymphoid and myeloid leukocytes. Rptor deletion unleashes differentiation of IMLECs from common myeloid progenitor cells by reducing expression of Myb. Importantly, IMLECs broadly overexpress pattern-recognition receptors and their expansion causes systemic inflammation in response to Toll-like receptor ligands in mice. Our data unveil a novel leukocyte population and an unrecognized role of Raptor/mTORC1 in innate immune tolerance