Heat transfer in solar absorber plates with micro-channels

Analytical, computational and experimental studies were carried out to investigate heat transfer and fluid flow in micro-channel absorber plates for compact (thin and light-weight) solar thermal collectors. The main objective of the work was to study different design and/or operating scenarios as well as study the significance of various micro-scaling effects. Analytical investigation showed that, under similar conditions, the proposed design yields a much higher fin efficiency, F and collector efficiency factor, F’ compared with the conventional solar collector design. An analytical model combining convective heat transfer in the collector fluid with axial conduction in the metal plate was developed. The predicted plate temperature profiles from the analytical model were in close agreement with the measured profiles. The model further showed that axial thermal conduction can significantly alter the plate temperature profile. Experiments were designed to represent real life operation of the proposed system. A CFD study, using the same design and operating parameters, produced results comparable with experiments. This numerical simulation also gave further insight into the heat transfer and fluid flow patterns in the micro-channel plate. The effect of channel cross section geometry was studied. The Nusselt number was observed to increase as the aspect ratio approached unity. Measured friction factors were similar in trend to the predictions for rectangular channels, although the overall rise in fluid temperature resulted in slightly lower friction factors. Thermal performance reduced slightly with increase in hydraulic diameter. The significance of various scaling effects was also investigated experimentally and numerically. Most of the typical scaling effects such as viscous dissipation and entrance effects were found to be insignificant however, conjugate heat transfer, surface boundary condition, surface finish and measurement uncertainties could be significant. The results showed a Reynolds number dependent Nusselt number which has been attributed to axial thermal conduction. It was also observed that only three walls were transferring heat; the walls of heat transfer had a uniform peripheral temperature while the heat flux varied peripherally. The closest simplified thermal boundary condition to represent heat transfer in these channels is the H1 with three (3) walls transferring heat. Increased surface roughness (obtained by using an etching technique to create the channels) was found to have a detrimental effect on heat transfer. The results showed that thermal improvement can be achieved by increasing the fluid velocity; however, pumping the thermal fluid above a pump power per plate area of 0.3 W/m2 resulted in marginal improvement. In practice, optimum microchannel geometry in plates should be sized based on fluid properties and operating conditions. The micro-channels should also have thin walls to minimise the effects of conjugate heat transfer. A Photovoltaic pump should be installed alongside the collector in order to provide pumping power required and minimise the overall fluid temperature rise. The results are beneficial for the design of micro-channel absorber plates for low heat flux operation up to 1000W/m2

Simple methodology for the quantitative analysis of fatty acids in human red blood cells

In the last years, there has been an increasing interest in evaluating possible relations between fatty acid (FA) patterns and the risk for chronic diseases. Due to the long life span (120 days) of red blood cells (RBCs), their FA profile reflects a longer term dietary intake and was recently suggested to be used as an appropriate biomarker to investigate correlations between FA metabolism and diseases. Therefore, the aim of this work was to develop and validate a simple and fast methodology for the quantification of a broad range of FAs in RBCs using gas chromatography with flame ionization detector, as a more common and affordable equipment suitable for biomedical and nutritional studies including a large number of samples. For this purpose, different sample preparation protocols were tested and compared, including a classic two-step method (Folch method) with modifications and different one-step methods, in which lipid extraction and derivatization were performed simultaneously. For the one-step methods, different methylation periods and the inclusion of a saponification reaction were evaluated. Differences in absolute FA concentrations were observed among the tested methods, in particular for some metabolically relevant FAs such as trans elaidic acid and eicosapentaenoic acid. The one-step method with saponification and 60 min of methylation time was selected since it allowed the identification of a higher number of FAs, and was further submitted to in-house validation. The proposed methodology provides a simple, fast and accurate tool to quantitatively analyse FAs in human RBCs, useful for clinical and nutritional studies.This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e Tecnologia) through project PTDC/SAU-ENB/116929/2010 and EXPL/EMS-SIS/2215/2013. ROR acknowledges PhD scholarship SFRH/BD/97658/2013 attributed by FCT (Fundação para a Ciência e Tecnologia).info:eu-repo/semantics/publishedVersio

Microwave Energy Increases Fatty Acid Methyl Ester Yield in Human Whole Blood Due to Increased Sphingomyelin Transesterification

Dried blood spots (DBS) by fingertip prick collection for fatty acid profiling are becoming increasingly popular due to ease of collection, minimal invasiveness and its amenability to high-throughput analyses. Herein, we assess a microwave-assisted direct transesterification method for the production of fatty acid methyl esters (FAME) from DBS. Technical replicates of human whole blood were collected and 25-μL aliquots were applied to chromatography strips prior to analysis by a standard 3-h transesterification method or microwave-assisted direct transesterification method under various power (variable vs constant), time (1-5 min) and reagent (1-10% H2SO4 in methanol) conditions. In addition, a standard method was compared to a 5-min, 30-W power microwave in 1% H2SO4 method for FAME yield from whole blood sphingomyelin, and sphingomyelin standards alone and spiked in whole blood. Microwave-assisted direct transesterification yielded no significant differences in both quantitative (nmol/100 µL) and qualitative (mol%) fatty acid assessments after as little as 1.5- and 1-min reaction times, respectively, using the variable power method and 5% H2SO4 in methanol. However, 30-W power for 5 min increased total FAME yield of the technical replicates by 14%. This increase appears largely due to higher sphingomyelin-derived FAME yield of up to 109 and 399% compared to the standard method when determined from whole blood or pure standards, respectively. In conclusion, microwave-assisted direct transesterification of DBS achieved in as little as 1-min, and 5-min reaction times increase total fatty acids primarily by significantly improving sphingomyelin-derived fatty acid yield

Intestinal microbiota in human health and disease: the impact of probiotics

The complex communities of microorganisms that colonise the human gastrointestinal tract play an important role in human health. The development of culture-independent molecular techniques has provided new insights in the composition and diversity of the intestinal microbiota. Here, we summarise the present state of the art on the intestinal microbiota with specific attention for the application of high-throughput functional microbiomic approaches to determine the contribution of the intestinal microbiota to human health. Moreover, we review the association between dysbiosis of the microbiota and both intestinal and extra-intestinal diseases. Finally, we discuss the potential of probiotic microorganism to modulate the intestinal microbiota and thereby contribute to health and well-being. The effects of probiotic consumption on the intestinal microbiota are addressed, as well as the development of tailor-made probiotics designed for specific aberrations that are associated with microbial dysbiosis