1,156 research outputs found
Brefeldin A and Exo1 Completely Releave the Block of Cholera Toxin Action by a Dipeptide Metalloendoprotease Substrate
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Quantitative SEM/EDS analysis of high-level waste glasses
Silicate glass will be used to stabilize high-level radioactive wastes (HLW) for disposal in a geological repository. The chemical durability of waste glass in the repository will be determined by the nature and progress of the glass-water (underground water) reaction. Aqueous corrosion of HLW glasses is known to result in the chemical alteration of the glass surface and the formation of secondary phases. Accurate quantitative analysis the altered glass surface and the reaction products that form is important for understanding the reaction kinetics and mechanism of glass corrosion. SEM/EDS is a powerful tool to study the alteration of waste-disposal glasses through quantitatively characterizing the reaction products. It is able to detect trace elements at concentrations less than 1 wt.%: for example, Fig. 1 shows the uranium peaks detected by SEM/EDS of a typical simulated waste glass, which contains 0.25 wt.% uranium in the matrix glass according to ICP/MS analysis. Nickel (0.44 wt.%) and chromium (0.47 wt.%) peaks are also clearly distinguishable in the spectrum. However, quantitative analyses of glass compositions are complicated by the lack of well-characterized reference samples having known and homogeneous compositions for candidate waste glasses, which may contain a combination of more than 20 different elements in one matrix. It has been proposed that unreacted glass with known compositions could satisfactorily serve as standards for quantitative analysis of waste-disposal glasses and the secondary phases. The main advantage of using such standards is that they contain all the elements to be analyzed in the homogeneous vitreous matrices. In this paper, we evaluate several reference waste glasses with respect to their suitability as standard samples for quantitative SEM/EDS analysis. The precision and accuracy using such standards for quantitative analysis are also discussed. All the experiments were carried out on a Topeon ABT 60 SEM operating at 20 kV
Novel RNA modifications in the nervous system: form and function
Modified RNA molecules have recently been shown to regulate nervous system functions. This mini-review and associated mini-symposium provide an overview of the types and known functions of novel modified RNAs in the nervous system, including covalently modified RNAs, edited RNAs, and circular RNAs. We discuss basic molecular mechanisms involving RNA modifications as well as the impact of modified RNAs and their regulation on neuronal processes and disorders, including neural fate specification, intellectual disability, neurodegeneration, dopamine neuron function, and substance use disorders
Non-Markovian dynamics for an open two-level system without rotating wave approximation: Indivisibility versus backflow of information
By use of the two measures presented recently, the indivisibility and the
backflow of information, we study the non-Markovianity of the dynamics for a
two-level system interacting with a zero-temperature structured environment
without using rotating wave approximation (RWA). In the limit of weak coupling
between the system and the reservoir, and by expanding the time-convolutionless
(TCL) generator to the forth order with respect to the coupling strength, the
time-local non-Markovian master equation for the reduced state of the system is
derived. Under the secular approximation, the exact analytic solution is
obtained and the sufficient and necessary conditions for the indivisibility and
the backflow of information for the system dynamics are presented. In the more
general case, we investigate numerically the properties of the two measures for
the case of Lorentzian reservoir. Our results show the importance of the
counter-rotating terms to the short-time-scale non-Markovian behavior of the
system dynamics, further expose the relations between the two measures and
their rationality as non-Markovian measures. Finally, the complete positivity
of the dynamics of the considered system is discussed
Spin diffusion at finite electric and magnetic fields
Spin transport properties at finite electric and magnetic fields are studied
by using the generalized semiclassical Boltzmann equation. It is found that the
spin diffusion equation for non-equilibrium spin density and spin currents
involves a number of length scales that explicitly depend on the electric and
magnetic fields. The set of macroscopic equations can be used to address a
broad range of the spin transport problems in magnetic multilayers as well as
in semiconductor heterostructure. A specific example of spin injection into
semiconductors at arbitrary electric and magnetic fields is illustrated
Spectral analysis and zeta determinant on the deformed spheres
We consider a class of singular Riemannian manifolds, the deformed spheres
, defined as the classical spheres with a one parameter family of
singular Riemannian structures, that reduces for to the classical metric.
After giving explicit formulas for the eigenvalues and eigenfunctions of the
metric Laplacian , we study the associated zeta functions
. We introduce a general method to deal with some
classes of simple and double abstract zeta functions, generalizing the ones
appearing in . An application of this method allows to
obtain the main zeta invariants for these zeta functions in all dimensions, and
in particular and . We give
explicit formulas for the zeta regularized determinant in the low dimensional
cases, , thus generalizing a result of Dowker \cite{Dow1}, and we
compute the first coefficients in the expansion of these determinants in powers
of the deformation parameter .Comment: 1 figur
Urinary active transforming growth factor ß in feline chronic kidney disease
The cytokine transforming growth factor beta 1 (TGF-β1) has been widely implicated in the development and progression of renal fibrosis in chronic kidney disease (CKD) in humans and in experimental models. The aims of this study were to assess the association between urinary active TGF-β1 and (a) development of CKD in a cross-sectional study, (b) deterioration of renal function over 1 year in a longitudinal study, and (c) renal histopathological parameters in cats. A human active TGF-β1 ELISA was validated for use in feline urine.
Cross-sectional analysis revealed no significant difference in urinary active TGF-β1:creatinine ratio (aTGF-β1:UCr) between groups with differing renal function. Longitudinally, non-azotaemic cats that developed CKD demonstrated a significant (P = 0.028) increase in aTGF-β1:UCr approximately 6 months before the development of azotaemia, which remained elevated (P = 0.046) at diagnosis (approximately 12 months prior, 8.4 pg/mg; approximately 6 months prior, 22.2 pg/mg; at CKD diagnosis, 24.6 pg/mg). In the histopathology study, aTGF-β1:UCr was significantly higher in cats with moderate (P = 0.02) and diffuse (P = 0.005) renal fibrosis than in cats without fibrosis. Cats with moderate renal inflammation had significantly higher urinary active aTGF-β1 concentrations than cats with mild (P = 0.035) or no inflammatory change (P = 0.004). The parameter aTGF-β1:UCr was independently associated with Log urine protein:creatinine ratio in a multivariable analysis of clinicopathological parameters and interstitial fibrosis score in a multivariable analysis of histopathological features. These results suggest that urinary aTGF-β1 reflects the severity of renal pathology. Increases in urinary aTGF-β1 followed longitudinally in individual cats may indicate the development of CKD
Time-Dependent Spintronic Transport and Current-Induced Spin Transfer Torque in Magnetic Tunnel Junctions
The responses of the electrical current and the current-induced spin transfer
torque (CISTT) to an ac bias in addition to a dc bias in a magnetic tunnel
junction are investigated by means of the time-dependent nonquilibrium Green
function technique. The time-averaged current (time-averaged CISTT) is
formulated in the form of a summation of dc current (dc CISTT) multiplied by
products of Bessel functions with the energy levels shifted by . The tunneling current can be viewed as to happen between the photonic
sidebands of the two ferromagnets. The electrons can pass through the barrier
easily under high frequencies but difficultly under low frequencies. The tunnel
magnetoresistance almost does not vary with an ac field. It is found that the
spin transfer torque, still being proportional to the electrical current under
an ac bias, can be changed by varying frequency. Low frequencies could yield a
rapid decrease of the spin transfer torque, while a large ac signal leads to
both decrease of the electrical current and the spin torque. If only an ac bias
is present, the spin transfer torque is sharply enhanced at the particular
amplitude and frequency of the ac bias. A nearly linear relation between such
an amplitude and frequency is observed.Comment: 13 pages,8 figure
Isotopic Insights into Biological Regulation of Zinc in Contaminated Systems
AbstractAquatic organisms use a variety ofbiogeochemical reactions to regulate essential and non-essential trace metals. Many of these mechanisms can lead to isotopic fractionation, thus measurement of metal isotopes may yield insights into the processes by which organisms respond to metal exposure. We illustrate these concepts with two case studies, one involving an intra- and the other an extra-cellular mechanism of Zn sequestration. In the first study, the mayflyNeocloeontrianguliferwas grown in the laboratory, and fed a diet of Zn-doped diatoms at Zn levels exceeding the requirements for normal mayfly life functions. The N. trianguliferlarvae consumed the diatoms and retained their Zn isotopic signature. Upon metamorphosis, the subimago life stage lost Zn mass either in the exuvia or by excretion, and the Zn retainedwas isotopically enriched. Thus, Zn uptake is non-fractionating, but Zn regulation favors the lighter isotope. Thus the Zn remaining in the subimago was isotopically heavier. In the second study, Zn was adsorbed on the cell walls and exopolysaccharide secretions of cyanobacteria, which favored the heavier Zn isotope.Continued adsorption eventually resulted in nucleation and biomineralization of hydrozincite {Zn5(CO3)2(OH)6}. These case studies demonstrate the utility of Zn isotopes to provide insights into how aquatic insects respond to metal exposure
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