25 research outputs found
Extracellular Matrix Aggregates from Differentiating Embryoid Bodies as a Scaffold to Support ESC Proliferation and Differentiation
Embryonic stem cells (ESCs) have emerged as potential cell sources for tissue engineering and regeneration owing to its virtually unlimited replicative capacity and the potential to differentiate into a variety of cell types. Current differentiation strategies primarily involve various growth factor/inducer/repressor concoctions with less emphasis on the substrate. Developing biomaterials to promote stem cell proliferation and differentiation could aid in the realization of this goal. Extracellular matrix (ECM) components are important physiological regulators, and can provide cues to direct ESC expansion and differentiation. ECM undergoes constant remodeling with surrounding cells to accommodate specific developmental event. In this study, using ESC derived aggregates called embryoid bodies (EB) as a model, we characterized the biological nature of ECM in EB after exposure to different treatments: spontaneously differentiated and retinoic acid treated (denoted as SPT and RA, respectively). Next, we extracted this treatment-specific ECM by detergent decellularization methods (Triton X-100, DOC and SDS are compared). The resulting EB ECM scaffolds were seeded with undifferentiated ESCs using a novel cell seeding strategy, and the behavior of ESCs was studied. Our results showed that the optimized protocol efficiently removes cells while retaining crucial ECM and biochemical components. Decellularized ECM from SPT EB gave rise to a more favorable microenvironment for promoting ESC attachment, proliferation, and early differentiation, compared to native EB and decellularized ECM from RA EB. These findings suggest that various treatment conditions allow the formulation of unique ESC-ECM derived scaffolds to enhance ESC bioactivities, including proliferation and differentiation for tissue regeneration applications. © 2013 Goh et al
Prescribed Burning of Thinning Slash in Regrowth Stands of Karri (Eucalyptus diversicolor) .1. Fire Characteristics, Fuel Consumption and Tree Damage
Formulation of a standardized protocol and determination of the size and appearance of the spleen in healthy cats
Recovery of Tall Open Eucalypt Forest in South-Western Australia following Complete Crown Scorch
Climate change, fuel and fire behaviour in a eucalypt forest
A suite of models was used to examine the links between climate, fuels and fire behaviour in dry eucalypt forests in south-eastern Australia. Predictions from a downscaled climate model were used to drive models of fuel amount, the moisture content of fuels and two models of forest fire behaviour at a location in western Sydney in New South Wales, Australia. We found that a warming and drying climate produced lower fine fuel amounts, but greater availability of this fuel to burn due to lower moisture contents. Changing fuel load had only a small effect on fuel moisture. A warmer, drier climate increased rate of spread, an important measure of fire behaviour. Reduced fuel loads ameliorated climate-induced changes in fire behaviour for one model. Sensitivity analysis of the other fire model showed that changes in fuel amount induced changes in fire behaviour of a similar magnitude to that caused directly by sensitivity to climate. Projection of changes in fire risk requires modelling of changes in vegetation as well as changes in climate. Better understanding of climate change effects on vegetation structure is required