Computed Tomography-guided fine-needle aspiration and tissue-core biopsy of bone lesions in small animals

Nell'uomo, la biopsia TC-guidata a mano libera è un metodo accurato per ottenere dei campioni di tessuto. Ci sono pochi lavori di questa tecnica in medicina veterinaria. Nel presente studio, 21 cani e 2 gatti sono stati sottoposti a biopsia TC-guidata (17 animali) o ago aspirato fine TC-guidato (6 animali) di lesioni ossee. Due delle 17 biospie sono state anche sottoposta ad esame colturale. Tutte le 17 biospie erano diagnostiche (accuratezza 100%). Cinque dei sei aghi aspirati fini erano diagnostici (accuratezza 83.3%). L'accuratezza totale era 95.7%. In un aspirato, la qualità citologica è stata considerata insufficiente in quanto conteneva solo sangue. Non si sono evidenziate complicazioni maggiori. Sono state diagnosticate 14 lesioni neoplastiche, 2 infezioni, e 6 lesioni benigne. L'esame TC dopo somministrazione di mezzo di cotnrasto endovenoso ha aggiunto informazioni utili per evitare grossi vasi e per biopsare tessuto vitale. La bipsia e l'ago aspirato fine TC-guidati a mano libera sembra essere una tecnica sicura e molto accurata per la diagnosi di patologie ossee nei piccoli animaliIn humans, free-hand computed tomography (CT)-guided biopsy is an accurate method to obtain a tissue sample. There are only a few reports of this technique in veterinary medicine. In the present study, 21 dogs and two cats underwent a free-hand CT-guided tissue-core biopsy (17 animals) or fine-needle aspiration (six animals) of a bone lesion. Two out of 17 tissue-core samples were also cultured. All 17 tissue-core biopsy samples were diagnostic (accuracy of 100%). Five out of six aspirates were diagnostic (accuracy of 83.3%). The overall accuracy was 95.7%. In one aspirate, cytologic quality was insufficient containing only blood. No major complications were encountered. Fourteen neoplastic, two infectious and six benign lesions were diagnosed. CT examination after intravenous contrast medium added useful information to avoid large vessels and to biopsy-viable tissue. Free-hand CT-guided tissue-core biopsy and aspiration appears to be a safe and very accurate procedure for use in the diagnosis of bone-associated diseases in small animal

Detecting short-term evolution of Etnean scoria cones: a LIDAR-based approach

The 2001 and 2002–2003 flank eruptions on Mount Etna (Italy) were characterized by intense explosive activity which led to the formation of two large monogenetic scoria cones (one from each eruption) on the upper southern flank of the volcano. Continuous monitoring of Etna, especially during flank eruptions, has provided detailed information on the growth of these cones. They differ in genesis, shape, and size. A set of high resolution (1 m) digital elevation models (DEMs) derived from light detection and ranging (LIDAR) data collected during four different surveys (2004, 2005, 2006, and 2007) has been used to map morphology and to extract the morphometric parameters of the scoria cones. By comparing LIDARderived DEMs with a pre-eruption (1998) 10 m DEM, the volume of the two scoria cones was calculated for the first time. Comparison of the LIDAR-derived DEMs revealed in unprecedented detail morphological changes during scoria cone degradation. In particular, the morphologically more exposed and structurally weaker 2002–2003 cone was eroded rapidly during the first few years after its emplacement mainly due to gravitational instability of slopes and wind erosion

Genomic screening identifies novel linkages and provides further evidence for a role of MYH9 in nonsyndromic cleft lip and palate

Nonsyndromic cleft lip with or without cleft palate (NSCLP) is a common birth anomaly that requires prolonged multidisciplinary rehabilitation. Although variation in several genes has been identified as contributing to NSCLP, most of the genetic susceptibility loci have yet to be defined. To identify additional contributory genes, a high-throughput genomic scan was performed using the Illumina Linkage IVb Panel platform. We genotyped 6008 SNPs in nine non-Hispanic white NSCLP multiplex families and a single large African-American NSCLP multiplex family. Fourteen chromosomal regions were identified with LOD>1.5, including six regions not previously reported. Analysis of the data from the African-American and non-Hispanic white families revealed two likely chromosomal regions: 8q21.3–24.12 and 22q12.2–12.3 with LOD scores of 2.98 and 2.66, respectively. On the basis of biological function, syndecan 2 (SDC2) and growth differentiation factor 6 (GDF6) in 8q21.3–24.12 and myosin heavy-chain 9, non-muscle (MYH9) in 22q12.2–12.3 were selected as candidate genes. Association analyses from these genes yielded marginally significant P-values for SNPs in SDC2 and GDF6 (0.01≤P<0.05). Evidence for an altered transmission was found for four MYH9 SNPs (P<0.01). SNP rs1002246 exhibited altered transmission by all analytic methods. However, analysis of two SNP MYH9 haplotypes did not identify a single high-risk haplotype. Our results confirm a previous report that 8q21.3–24.12 may harbor a clefting gene and identify 22q12.2–12.3 as a new candidate region that contains MYH9. Most importantly, we confirm the previous report of an association with MYH9