Exploring the functional domain and the target of the tetanus toxin light chain in neurohypophysial terminals

The tetanus toxin light chain blocks calcium induced vasopressin release from neurohypophysial nerve terminals. Here we show that histidine residue 233 within the putative zinc binding motif of the tetanus toxin light chain is essential for the inhibition of exocytosis, in the rat. The zinc chelating agent dipicolinic acid as well as captopril, an inhibitor of zinc-dependent peptidases, counteract the effect of the neurotoxin. Synthetic peptides, the sequences of which correspond to motifs present in the cytoplasmic domain of the synaptic vesicle membrane protein synaptobrevin 1 and 2, prevent the effect of the tetanus toxin light chain. Our results indicate that zinc bound to the zinc binding motif constitutes the active site of the tetanus toxin light chain. Moreover they suggest that cleavage of synaptobrevin by the neurotoxin causes the inhibition of exocytotic release of vasopressin from secretory granules

Deuteron Compton Scattering in Effective Field Theory: Spin-Dependent Cross Sections and Asymmetries

Polarized Compton scattering on the deuteron is studied in nuclear effective field theory. A set of tensor structures is introduced to define 12 independent Compton amplitudes. The scalar and vector amplitudes are calculated up to ${\cal O}((Q/\Lambda)^2)$ in low-energy power counting. Significant contribution to the vector amplitudes is found to come from the spin-orbit type of relativistic corrections. A double-helicity dependent cross section $\Delta_1 \sigma = (\sigma_{+1-1}-\sigma_{+1+1})/2$ is calculated to the same order, and the effect of the nucleon isoscalar spin-dependent polarizabilities is found to be smaller than the effect of isoscalar spin-independent ones. Contributions of spin-independent polarizabilities are investigated in various asymmetries, one of which has as large as 12 (26) percent effect at the center-of-mass photon energy 30 (50) MeV.Comment: 22 pages, 8 figures included, replaced with the version submitted to PR

Stable ultrahigh-density magneto-optical recordings using introduced linear defects

The stability of data bits in magnetic recording media at ultrahigh densities is compromised by thermal `flips' -- magnetic spin reversals -- of nano-sized spin domains, which erase the stored information. Media that are magnetized perpendicular to the plane of the film, such as ultrathin cobalt films or multilayered structures, are more stable against thermal self-erasure than conventional memory devices. In this context, magneto-optical memories seem particularly promising for ultrahigh-density recording on portable disks, and bit densities of $\sim$100 Gbit inch$^{-2}$ have been demonstrated using recent advances in the bit writing and reading techniques. But the roughness and mobility of the magnetic domain walls prevents closer packing of the magnetic bits, and therefore presents a challenge to reaching even higher bit densities. Here we report that the strain imposed by a linear defect in a magnetic thin film can smooth rough domain walls over regions hundreds of micrometers in size, and halt their motion. A scaling analysis of this process, based on the generic physics of disorder-controlled elastic lines, points to a simple way by which magnetic media might be prepared that can store data at densities in excess of 1 Tbit inch$^{-2}$.Comment: 5 pages, 4 figures, see also an article in TRN News at http://www.trnmag.com/Stories/041801/Defects_boost_disc_capacity_041801.htm

The Effect of Training on Stride Duration in a Cohort of Two-Year-Old and Three-Year-Old Thoroughbred Racehorses

Objective gait monitoring is increasingly accessible to trainers. A more comprehensive understanding of ‘normal’ gait adaptations is required. Forty two-year-old thoroughbred racehorses were recruited when entering training and followed for 22 months. Gait analysis was performed by equipping each horse with an inertial measurement unit with inbuilt GPS (GPS-IMU) mounted on the dorsum. Horses were exercised as per their regular training regimen. Data were analysed using a linear mixed model. For two-year-old horses, there was a non-linear pattern of stride duration (SD) over time (p < 0.001) with SD decreasing initially and then ‘flattening off’ over time (linear and quadratic coefficients −0.29 ms/week and 0.006 ms/week2). Horses showed an increase in SD of 2.21 ms (p < 0.001) per 100 m galloped, and over time, SD decreased by 0.04 ms (p < 0.001) with each 100 m galloped per week. Three-year-old horses overall showed no change in SD over time (p = 0.52), but those that had a period of time off showed a decrease in SD of −0.59 ms per week (p = 0.02). They showed an increase in SD of 1.99 ms (p < 0.001) per 100 m galloped, and horses that had a period of time off showed an increase in stride duration of 1.05 ms per 100 m galloped (p = 0.01) compared to horses which did not have time off. Horses demonstrate an adaptation to high-speed exercise over time. SD decreases with training when other factors are controlled for in naïve horses

Domain Dynamics of Magnetic Films with Perpendicular Anisotropy

We study the magnetic properties of nanoscale magnetic films with large perpendicular anisotropy comparing polarization microscopy measurements on Co_28Pt_72 alloy samples based on the magneto-optical Kerr effect with Monte Carlo simulations of a corresponding micromagnetic model. We focus on the understanding of the dynamics especially the temperature and field dependence of the magnetisation reversal process. The experimental and simulational results for hysteresis, the reversal mechanism, domain configurations during the reversal, and the time dependence of the magnetisation are in very good qualitative agreement. The results for the field and temperature dependence of the domain wall velocity suggest that for thin films the hysteresis can be described as a depinning transition of the domain walls rounded by thermal activation for finite temperatures.Comment: 7 pages Latex, Postscript figures included, accepted for publication in Phys.Rev.B, also availible at: http://www.thp.Uni-Duisburg.DE/Publikationen/Publist_Us_R.htm

Singlet Ground State and Magnetization Plateaus in Ba3_3Mn2_2O8_8

Magnetic susceptibility and the magnetization process have been measured in \green polycrystal. In this compound, the magnetic manganese ion exists as Mn$^{5+}$ in a tetrahedral environment, and thus the magnetic interaction can be described by an S=1 Heisenberg model. The ground state was found to be a spin singlet with an excitation gap $\Delta/k_{\rm B}=11.2$ K. Magnetization plateaus were observed at zero and at half of the saturation magnetization. These results indicate that the present system can be represented by a coupled antiferromagnetic dimer model.Comment: 4 pages, 4 figures, jpsj styl

Tension-tension testing of a novel mooring rope construction

This is the author accepted manuscript.Synthetic fibre ropes are in widespread use in maritime applications ranging from lifting to temporary and permanent mooring systems for vessels, offshore equipment and platforms. The selection of synthetic ropes over conventional steel components is motivated by several key advantages including selectable axial stiffness, energy absorption (and hence load mitigation), fatigue resistance and low unit cost. The long-term use of ropes as safety critical components in potentially high dynamic loading environments necessitates that new designs are verified using stringent qualification procedures. The International Organization for Standardization (ISO) is one certification body that has produced several guidelines for the testing of synthetic ropes encompassing quasi-static and dynamic loading as well as fatigue cycling. This paper presents the results of tension-tension tests carried out to ISO 2307:2010, ISO 18692:2007(E) and ISO/TS 19336:2015(E) on three different 12-strand rope constructions manufactured by Ashimori Industry Co. Ltd from polyester and Vectran® fibres. The purpose of the tests was to characterise the performance of a novel 12-strand construction and compare this to a conventional 12-strand construction. Utilising the Dynamic Marine Component test facility (DMaC) at the University of Exeter several key performance metrics were determined including; elongation, minimum break load (MBL) and quasi-static and dynamic stiffness. During the ISO 2307:2010(E) test programme the samples were tested dry and during the ISO 18692:2007(E) and ISO/TS 19336:2015(E) test programmes the samples were fully submerged in tap water after being soaked for at least 24 hours. Two methods were used to quantify sample extension: i) an optical tracking system and ii) a draw-wire potentiometer. Axial compression fatigue and cyclic loading endurance tests were also carried out on two Vectran® samples. Further load-to-failure tests and sample analysis were also carried out by Ashimori Industry Co. Ltd. It was found that the MBL of the samples exceeded the values specified by the manufacturer (by 7.7-29.5% for the polyester samples) with failure occurring at the splices in all cases and minor abrasion noted in several locations. The measured MBL of the novel polyester Straight Strand Rope (SSR) construction was up to 16% higher than the conventional construction with increases of quasi-static and dynamic stiffness of up to 6.8%. Differences between the viscoelastic and viscoplastic behaviour of the samples were also noted. The data obtained during these tests will provide insight into the behaviour of these materials and different rope constructions which will be of use to rope manufacturers, mooring system designers in addition to offshore equipment and vessel operators.The authors at the University of Exeter would like to thank their colleagues at Nagasaki University and Ashimori Industry Co. Ltd for being given the opportunity to carry out the interesting work reported in this paper. Through the Peninsula Research Institute for Marine Renewable Energy (PRIMaRE) consortium, the DMaC test facility was funded from the ERDF Convergence programme and South West Regional Development Agenc