An investigation into the dynamics of vehicles with hydraulically interconnected suspensions

University of Technology, Sydney. Faculty of Engineering.This thesis examines the dynamics of a particular class of vehicle suspension, namely hydraulically interconnected suspension (HIS), often claimed to break the compromise between ride and handling performance. Yet such systems have, until quite recently, received little attention in the academic literature. Ideally, interconnected schemes have the capability, unique among passive suspensions, to provide stiffness and damping characteristics dependent on the all-wheel suspension mode of operation. The modelling approach proposed here is necessarily multidisciplinary, drawing from multi-body vibration theory and fluid dynamics. A simple half-car model is used to illustrate the basic principles and to demonstrate the application of the methodology. The half-car is treated as a lumped-mass multi-body system and the fluid circuits as continuous line elements. Individual fluid components are modelled using the impedance method, and the relationships between the fluid states at the extremities of each circuit are determined by the transfer matrix method. The resulting set of linear, frequency-dependent state-space equations, which govern the coupled dynamics of the half-car system, are derived and then applied in a variety of ways. This includes a free vibration analysis, ride comfort assessment and multi-objective optimisation. A number of key components that influence HIS performance are identified and a sensitivity analysis of their effects is presented. Validation of the theoretical modelling is performed in two ways. First, simulations of an identical half-car using an alternative, nonlinear finite element fluid model are conducted. Second, experiments with a unique, purpose-built, half-car test rig are performed. The free and forced vibration results obtained with both methods, in general, agree very well with the proposed linear model. The methodology presented is found to be an effective and useful way of modelling HIS- equipped vehicles, particularly in the frequency domain. The obtained results suggest that interconnected suspension schemes may provide, at least to some extent, an improved compromise between ride and handling. However, further investigation of this claim, including the development of a detailed full-car model, is recommended as a topic for future studies

Damage identification based on response-only measurements using cepstrum analysis and artificial neural networks

This article presents a response-only structural health monitoring technique that utilises cepstrum analysis and artificial neural networks for the identification of damage in civil engineering structures. The method begins by applying cepstrum-based operational modal analysis, which separates source and transmission path effects to determine the structure's frequency response functions from response measurements only. Principal component analysis is applied to the obtained frequency response functions to reduce the data size, and structural damage is then detected using a two-stage ensemble of artificial neural networks. The proposed method is verified both experimentally and numerically using a laboratory two-storey framed structure and a finite element representation, both subjected to a single excitation. The laboratory structure is tested on a large-scale shake table generating ambient loading of Gaussian distribution. In the numerical investigation, the same input is applied to the finite model, but the obtained responses are polluted with different levels of white Gaussian noise to better replicate real-life conditions. The damage is simulated in the experimental and numerical investigations by changing the condition of individual joint elements from fixed to pinned. In total, four single joint changes are investigated. The results of the investigation show that the proposed method is effective in identifying joint damage in a multi-storey structure based on response-only measurements in the presence of a single input. Because the technique does not require a precise knowledge of the excitation, it has the potential for use in online structural health monitoring. Recommendations are given as to how the method could be applied to the more general multiple-input case. © The Author(s) 2014

Proximate factors underpinning receiver responses to deceptive false alarm calls in wild tufted capuchin monkeys: is it counterdeception?

Previous research demonstrates that tufted capuchin monkeys use terrestrial predator alarm calls in a functionally deceptive manner to distract conspecifics when feeding on contestable resources, although the success of this tactic is limited because listeners frequently ignore these calls when given in such situations. While this decreased response rate is suggestive of a counterstrategy to deception by receivers, the proximate factors underpinning the behavior are unclear. The current study aims to test if the decreased response rate to alarm calls in competitive contexts is better explained by the perception of subtle acoustic differences between predator-elicited and deceptive false alarms, or by receivers varying their responses based on the context in which the signal is received. This was tested by first examining the acoustic structure of predator-elicited and deceptive false alarms for any potentially perceptible acoustic differences, and second by comparing the responses of capuchins to playbacks of each of predator-elicited and false alarms, played back in noncompetitive contexts. The results indicate that deceptive false alarms and predator-elicited alarms show, at best, minimal acoustic differences based on the structural features measured. Likewise, playbacks of deceptive false alarms elicited antipredator reactions at the same rate as did predator-elicited alarms, although there was a nonsignificant tendency for false alarms to be more likely to elicit escape reactions. The lack of robust acoustic differences together with the high response rate to false alarms in noncompetitive contexts suggests that the context in which the signal is received best explains receiver responses. It remains unclear, however, if listeners ascribe different meanings to the calls based on context, or if they generally ignore all signals in competitive contexts. Whether or not the decreased response rate of receivers directly stems from the deceptive use of the calls cannot be determined until these latter possibilities are rigorously tested

A missing dimension in measures of vaccination impacts

Immunological protection, acquired from either natural infection or vaccination, varies among hosts, reflecting underlying biological variation and affecting population-level protection. Owing to the nature of resistance mechanisms, distributions of susceptibility and protection entangle with pathogen dose in a way that can be decoupled by adequately representing the dose dimension. Any infectious processes must depend in some fashion on dose, and empirical evidence exists for an effect of exposure dose on the probability of transmission to mumps-vaccinated hosts [1], the case-fatality ratio of measles [2], and the probability of infection and, given infection, of symptoms in cholera [3]. Extreme distributions of vaccine protection have been termed leaky (partially protects all hosts) and all-or-nothing (totally protects a proportion of hosts) [4]. These distributions can be distinguished in vaccine field trials from the time dependence of infections [5]. Frailty mixing models have also been proposed to estimate the distribution of protection from time to event data [6], [7], although the results are not comparable across regions unless there is explicit control for baseline transmission [8]. Distributions of host susceptibility and acquired protection can be estimated from dose-response data generated under controlled experimental conditions [9]–[11] and natural settings [12], [13]. These distributions can guide research on mechanisms of protection, as well as enable model validity across the entire range of transmission intensities. We argue for a shift to a dose-dimension paradigm in infectious disease science and community health

Fat levels analysis of mantis shrimp (harpiosquilla raphidea) in the interidal zone in the waters of the state of Suli, Central Maluku

Mantis shrimp (Harpiosquilla raphidea) is a type of predatory shrimp. Mantis shrimp is a type of crustacean with high nutritional value, but it is not widely known by the people of Eastern Indonesia, especially the people of Suli. This research was conducted in May 2021 and was tested for fat content at the Basic Chemistry Laboratory, Pattimura University, Ambon, with the type of descriptive research. This study used the Soxhlet method, which is the method used for fat extraction with N-Hexane as a fat solvent. The results of this study showed that the fat content of white mantis shrimp was 3.33% and black mantis shrimp was 5.56%. The fat content of white mantis shrimp is 3.33% and black mantis shrimp is 5.56%

Intraocular solitary extramedullary plasmacytoma presenting as unilateral anterior and intermediate uveitis preceded by refractory glaucoma

Background: Solitary extramedullary plasmacytoma (SEP) is a localised proliferation of monoclonal plasma cells involving soft tissue with no or minimal bone marrow involvement and no other systemic evidence of multiple myeloma. Intraocular involvement is exceedingly rare. Case presentation: We report a 78-year-old man who was referred with glaucoma in the right eye. He subsequently developed anterior chamber (AC) inflammation and refractory glaucoma then dense vitritis. A vitrectomy was performed with the biopsy revealing numerous plasma cells with atypical findings. In conjunction with the flow cytometry results, and a systemic work up excluding multiple myeloma, a diagnosis of SEP was made. The patient was treated with ocular external beam radiotherapy with resolution of the intraocular inflammation and control of the intraocular pressure. He remains well with no local recurrence and no development of multiple myeloma over a follow up period of 2.5 years. Conclusions: This is the first case report of SEP presenting as intraocular inflammation without a uveal tract mass

Quality control for diagnostic oral microbiology laboratories in European countries

Participation in diagnostic microbiology internal and external quality control (QC) processes is good laboratory practice and an essential component of a quality management system. However, no QC scheme for diagnostic oral microbiology existed until 2009 when the Clinical Oral Microbiology (COMB) Network was created. At the European Oral Microbiology Workshop in 2008, 12 laboratories processing clinical oral microbiological samples were identified. All these were recruited to participate into the study and six laboratories from six European countries completed both the online survey and the first QC round. Three additional laboratories participated in the second round. Based on the survey, European oral microbiology laboratories process a significant (mean per laboratory 4,135) number of diagnostic samples from the oral cavity annually. A majority of the laboratories did not participate in any internal or external QC programme and nearly half of the laboratories did not have standard operating procedures for the tests they performed. In both QC rounds, there was a large variation in the results, interpretation and reporting of antibiotic susceptibility testing among the laboratories. In conclusion, the results of this study demonstrate the need for harmonisation of laboratory processing methods and interpretation of results for oral microbiology specimens. The QC rounds highlighted the value of external QC in evaluating the efficacy and safety of processes, materials and methods used in the laboratory. The use of standardised methods is also a prerequisite for multi-centre epidemiological studies that can provide important information on emerging microbes and trends in anti-microbial susceptibility for empirical prescribing in oro-facial infections

ciliaFA : a research tool for automated, high-throughput measurement of ciliary beat frequency using freely available software

Background: Analysis of ciliary function for assessment of patients suspected of primary ciliary dyskinesia (PCD) and for research studies of respiratory and ependymal cilia requires assessment of both ciliary beat pattern and beat frequency. While direct measurement of beat frequency from high-speed video recordings is the most accurate and reproducible technique it is extremely time consuming. The aim of this study was to develop a freely available automated method of ciliary beat frequency analysis from digital video (AVI) files that runs on open-source software (ImageJ) coupled to Microsoft Excel, and to validate this by comparison to the direct measuring high-speed video recordings of respiratory and ependymal cilia. These models allowed comparison to cilia beating between 3 and 52 Hz. Methods: Digital video files of motile ciliated ependymal (frequency range 34 to 52 Hz) and respiratory epithelial cells (frequency 3 to 18 Hz) were captured using a high-speed digital video recorder. To cover the range above between 18 and 37 Hz the frequency of ependymal cilia were slowed by the addition of the pneumococcal toxin pneumolysin. Measurements made directly by timing a given number of individual ciliary beat cycles were compared with those obtained using the automated ciliaFA system. Results: The overall mean difference (± SD) between the ciliaFA and direct measurement high-speed digital imaging methods was −0.05 ± 1.25 Hz, the correlation coefficient was shown to be 0.991 and the Bland-Altman limits of agreement were from −1.99 to 1.49 Hz for respiratory and from −2.55 to 3.25 Hz for ependymal cilia. Conclusions: A plugin for ImageJ was developed that extracts pixel intensities and performs fast Fourier transformation (FFT) using Microsoft Excel. The ciliaFA software allowed automated, high throughput measurement of respiratory and ependymal ciliary beat frequency (range 3 to 52 Hz) and avoids operator error due to selection bias. We have included free access to the ciliaFA plugin and installation instructions in Additional file 1 accompanying this manuscript that other researchers may use