THREE-DIMENSIONAL FINGERPRINT SPECTROSCOPY STUDY ON THE BIOPOLYMER SYSTEM OF POLYPHENOL OXIDASE BINDING WITH CUMALIC ACID

The protection of Cumalic acid (CA), antioxidant, in the biochemical process in nature has aroused great interest. Polyphenol oxidase (PPO), an enzyme, plays a vital function in aging and browning of plants, such as vegetables, fruits, and mushrooms. The interaction of CA and PPO reveals the important information in metabolism and aging. Thus, the molecular mechanism of CA binding with polyphenol oxidase (PPO) was explored by combining spectroscopic methods with molecular modeling. A three-dimensional fingerprint of the CA-PPO complex was built for the first time to characterize the biopolymer interaction between CA and PPO. Application of the spectroscopic methods indicated that CA effectively quenched the intrinsic fluorescence of PPO. The enthalpy change (ΔH°) and entropy change (ΔS°) suggested that the CA-PPO complex was predominantly stabilized by hydrophobic interactions CA and PPO. Building the λ-UV-F fingerprint of CA-PPO made it possible to demonstrate the three-dimensional interactions between CA and PPO. Subsequently, molecular modeling demonstrated that CA was primarily bound to PPO by hydrophobic interactions and hydrogen bonds located at amino acid residues Ala202, His38, His54, and Ser206. The computational simulations were consistent with the spectral experiments demonstrating confidence in the three-dimensional model determined of the CA-PPO interaction

The trans-ancestral genomic architecture of glycemic traits

Glycemic traits are used to diagnose and monitor type 2 diabetes and cardiometabolic health. To date, most genetic studies of glycemic traits have focused on individuals of European ancestry. Here we aggregated genome-wide association studies comprising up to 281,416 individuals without diabetes (30% non-European ancestry) for whom fasting glucose, 2-h glucose after an oral glucose challenge, glycated hemoglobin and fasting insulin data were available. Trans-ancestry and single-ancestry meta-analyses identified 242 loci (99 novel; P < 5 x 10(-8)), 80% of which had no significant evidence of between-ancestry heterogeneity. Analyses restricted to individuals of European ancestry with equivalent sample size would have led to 24 fewer new loci. Compared with single-ancestry analyses, equivalent-sized trans-ancestry fine-mapping reduced the number of estimated variants in 99% credible sets by a median of 37.5%. Genomic-feature, gene-expression and gene-set analyses revealed distinct biological signatures for each trait, highlighting different underlying biological pathways. Our results increase our understanding of diabetes pathophysiology by using trans-ancestry studies for improved power and resolution.A trans-ancestry meta-analysis of GWAS of glycemic traits in up to 281,416 individuals identifies 99 novel loci, of which one quarter was found due to the multi-ancestry approach, which also improves fine-mapping of credible variant sets.Diabetes mellitus: pathophysiological changes and therap

Aperture Effects on Charged-Particles in a Magnetic-Field

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Agricultural ammonia emissions inventory and spatial distribution in the North China Plain

An agricultural ammonia (NH3) emission inventory in the North China Plain (NCP) on a prefecture level for the year 2004, and a 5 × 5 km resolution spatial distribution map, have been calculated for the first time. The census database from China’s statistics datasets, and emission factors re-calculated by the RAINS model supported total emissions of 3071 kt NH3-N yr-1 for the NCP, accounting for 27% of the total emissions in China. NH3 emission from mineral fertilizer application contributed 1620 kt NH3-N yr-1, 54% of the total emission, while livestock emissions accounted for the remaining 46% of the total emissions, including 7%, 27%, 7% and 5% from cattle, pigs, sheep and goats, and poultry, respectively. A high-resolution spatial NH3 emissions map was developed based on 1 × 1 km land use database and aggregated to a 5 × 5 km grid resolution. The highest emission density value was up to 198 kg N ha-1 yr-1

An improved capacitive divider probe for plasma potential measurements in the Phaedrus tokamak

This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder