The Effect of Wavelet Families on Watermarking

With the advance of technologies such as the Internet, Wi-Fi Internet availability and mobile access, it is becoming harder than ever to safeguard intellectual property in a digital form. Digital watermarking is a steganographic technique that is used to protect creative content. Copyrighted work can be accessed from many different computing platforms; the same image can exist on a handheld personal digital assistant, as well as a laptop and desktop server computer. For those who want to pirate, it is simple to copy, modify and redistribute digital media. Because this impacts business profits adversely, this is a highly researched field in recent years. This paper examines a technique for digital watermarking which utilizes properties of the Discrete Wavelet Transform (DWT). The digital watermarking algorithm is explained. This algorithm uses a database of 40 images that are of different types. These images, including greyscale, black and white, and color, were chosen for their diverse characteristics. Eight families of wavelets, both orthogonal and biorthogonal, are compared for their effectiveness. Three distinct watermarks are tested. Since compressing an image is a common occurrence, the images are compacted to determine the significance of such an action. Different types of noise are also added. The PSNR for each image and each wavelet family is used to measure the efficacy of the algorithm. This objective measure is also used to determine the influence of the mother wavelet. The paper asks the question: “Is the wavelet family chosen to implement the algorithm of consequence?” In summary, the results support the concept that the simpler wavelet transforms, e.g. the Haar wavelet, consistently outperform the more complex ones when using a non-colored watermark

Exact Solution of the Infinite-Range Quantum Mattis Model

We have solved the quantum version of the Mattis model with infinite-range interactions. A variational approach gives the exact solution for the infinite-range system, in spite of the non-commutative nature of the quantum spin components; this implies that quantum effects are not predominant in determining the macroscopic properties of the system. Nevertheless, the model has a surprisingly rich phase behaviour, exhibiting phase diagrams with tricritical, three-phase and critical end points.Comment: 14 pages, 11 figure

TOBFAC: the database of tobacco transcription factors

<p>Abstract</p> <p>Background</p> <p>Regulation of gene expression at the level of transcription is a major control point in many biological processes. Transcription factors (TFs) can activate and/or repress the transcriptional rate of target genes and vascular plant genomes devote approximately 7% of their coding capacity to TFs. Global analysis of TFs has only been performed for three complete higher plant genomes – Arabidopsis (<it>Arabidopsis thaliana</it>), poplar (<it>Populus trichocarpa</it>) and rice (<it>Oryza sativa</it>). Presently, no large-scale analysis of TFs has been made from a member of the <it>Solanaceae</it>, one of the most important families of vascular plants. To fill this void, we have analysed tobacco (<it>Nicotiana tabacum</it>) TFs using a dataset of 1,159,022 gene-space sequence reads (GSRs) obtained by methylation filtering of the tobacco genome. An analytical pipeline was developed to isolate TF sequences from the GSR data set. This involved multiple (typically 10–15) independent searches with different versions of the TF family-defining domain(s) (normally the DNA-binding domain) followed by assembly into contigs and verification. Our analysis revealed that tobacco contains a minimum of 2,513 TFs representing all of the 64 well-characterised plant TF families. The number of TFs in tobacco is higher than previously reported for Arabidopsis and rice.</p> <p>Results</p> <p>TOBFAC: the database of tobacco transcription factors, is an integrative database that provides a portal to sequence and phylogeny data for the identified TFs, together with a large quantity of other data concerning TFs in tobacco. The database contains an individual page dedicated to each of the 64 TF families. These contain background information, domain architecture via Pfam links, a list of all sequences and an assessment of the minimum number of TFs in this family in tobacco. Downloadable phylogenetic trees of the major families are provided along with detailed information on the bioinformatic pipeline that was used to find all family members. TOBFAC also contains EST data, a list of published tobacco TFs and a list of papers concerning tobacco TFs. The sequences and annotation data are stored in relational tables using a PostgrelSQL relational database management system. The data processing and analysis pipelines used the Perl programming language. The web interface was implemented in JavaScript and Perl CGI running on an Apache web server. The computationally intensive data processing and analysis pipelines were run on an Apple XServe cluster with more than 20 nodes.</p> <p>Conclusion</p> <p>TOBFAC is an expandable knowledgebase of tobacco TFs with data currently available for over 2,513 TFs from 64 gene families. TOBFAC integrates available sequence information, phylogenetic analysis, and EST data with published reports on tobacco TF function. The database provides a major resource for the study of gene expression in tobacco and the <it>Solanaceae </it>and helps to fill a current gap in studies of TF families across the plant kingdom. TOBFAC is publicly accessible at <url>http://compsysbio.achs.virginia.edu/tobfac/</url>.</p

Methamphetamine Causes Differential Alterations in Gene Expression and Patterns of Histone Acetylation/Hypoacetylation in the Rat Nucleus Accumbens

Methamphetamine (METH) addiction is associated with several neuropsychiatric symptoms. Little is known about the effects of METH on gene expression and epigenetic modifications in the rat nucleus accumbens (NAC). Our study investigated the effects of a non-toxic METH injection (20 mg/kg) on gene expression, histone acetylation, and the expression of the histone acetyltransferase (HAT), ATF2, and of the histone deacetylases (HDACs), HDAC1 and HDAC2, in that structure. Microarray analyses done at 1, 8, 16 and 24 hrs after the METH injection identified METH-induced changes in the expression of genes previously implicated in the acute and longterm effects of psychostimulants, including immediate early genes and corticotropin-releasing factor (Crf). In contrast, the METH injection caused time-dependent decreases in the expression of other genes including Npas4 and cholecystokinin (Cck). Pathway analyses showed that genes with altered expression participated in behavioral performance, cell-to-cell signaling, and regulation of gene expression. PCR analyses confirmed the changes in the expression of c-fos, fosB, Crf, Cck, and Npas4 transcripts. To determine if the METH injection caused post-translational changes in histone markers, we used western blot analyses and identified METH-mediated decreases in histone H3 acetylated at lysine 9 (H3K9ac) and lysine 18 (H3K18ac) in nuclear sub-fractions. In contrast, the METH injection caused time-dependent increases in acetylated H4K5 and H4K8. The changes in histone acetylation were accompanied by decreased expression of HDAC1 but increased expression of HDAC2 protein levels. The histone acetyltransferase, ATF2, showed significant METH-induced increased in protein expression. These results suggest that METH-induced alterations in global gene expression seen in rat NAC might be related, in part, to METH-induced changes in histone acetylation secondary to changes in HAT and HDAC expression. The causal role that HATs and HDACs might play in METH-induced gene expression needs to be investigated further

GEOLOGICAL SURVEY CIRCULAR 165 RAPID ANALYSIS OF SILICATE ROCKS RAPID ANALYSIS OF SILICATE ROCKS

ABSTRACT Rapid methods are described for the determination of the major constituents in silicate rocks. SiOg and AlgC^ are determined spectrophotometrically on aliquots of a solution prepared by fusion of the sample with NaOH. A molybdenum blue method is used for SiOg and f err on is used in the determination of A^OS. A second solution prepared by digestion of the sample with HF and HgSC^ is used for the determination of TiOg,&quot; total iron, MnO and PgO5&gt; by spectrophotometric methods. TiO2 is determined as the peroxide, total iron as ferric chloride, MnO as permanganate, and FgOg as molybdivanado phosphoric acid. Additional aliquots of the solution prepared after digestion with HF plus H2SO4 are used for the determination of CaO and MgO by titration methods, using versene. Another aliquot of the same solution is used for the determination of KgO and NagO by flame photometer. FeO is determined by titration with KgCrgOY after decomposition of the sample with HF and HgSO^.. Loss on ignition is determined so that a summation may be obtained and used as an aid in checking the reliability of the results. A schedule for rapid complete analysis of silicate rocks in sets of eight samples is given. Results of rapid analysis of twelve synthetic mixtures prepared from National Bureau of Standards standard samples are compared with values based on those reported by the Bureau of Standards. RAPID ANALYSIS OF SILICATE ROCKS CONTENT

Brain transcription factor expression: effects of acute and chronic amphetamine and injection stress

Amphetamine influences behaviors and the expression of transcription factor genes in the central nervous system (CNS). A single d-amphetamine dose (7.5 mg/kg, i.p.) enhances behavioral stereotypy and augments brain expression of c-fos, fos-B, fra-1, zif268, jun-B, and c-jun by 2-11 fold. When the single amphetamine dose is preceded by 28 saline injections over 14 days, it is half as effective in enhancing expression of these genes. Rats injected with 7.5 mg/kg i.p. twice daily for 2 weeks and sacrificed after the last injection reveal further attenuation or abolition of the amphetamine-induced mRNA upregulation. These stigmata of 'tolerance' in gene expression display partial overlap with behavioral tolerance, manifest as changes in locomotor activity. Rats receiving low (2 mg/kg) amphetamine challenge doses following the 2-week 7.5 mg/kg b.i.d. amphetamine treatment show tolerance to the locomotor activating effects of the drug; no tolerance is evident following a high (7.5 mg/kg) challenge dose. These data suggest that amphetamine-induced alterations in brain transcription factor gene expression can display 'tolerance' and possibly 'cross-tolerance' with the stress caused by i.p. injection