Germination differences between seeds from apical and basal fruits of "Camelina microcarpa" Andrz. ex DC. produced in two different climates

En el presente trabajo se han estudiado las diferencias de germinación entre semillas de una población de “Camelina microcarpa” Andrz. ex DC. producidas en los frutos apicales y basales de las mismas infrutescencias, y producidas en dos condiciones climáticas diferentes (Lleida y Minnesota). Las semillas apicales germinaron más y antes que las basales, principalmente cuando fueron producidas en Lleida, mientras que estas diferencias se difuminaron en las semillas procedentes de Minnesota; estas últimas semillas también germinaron en mayor medida que las de Lleida. A pesar de las diferencias, los ritmos de germinación fueron similares en casi todos los casos. Los resultados ponen de manifiesto la importancia del lugar de producción de las semillas de cara a su posible comercialización como cultivo oleaginoso.Germination characteristics of seeds collected from apical and basal fruits of the infrutescences, and produced in two different climatic conditions (Lleida and Minnesota) were studied. Apical seeds germinated more and earlier than basal seeds, mainly when they were produced in Lleida, while these differences blur between seeds produced in Minnesota, which germinated in a greater amount than those of Lleida. Despite these differences, germination rates were similar in all cases. The results show the importance of the seed production site if these species was considered as a potential commercial oilseed crop

Sistem Penilaian Kinerja Pegawai dengan Metode Analytical Hierarchy Process (Ahp) (Studi Kasus pada Kantor Stasiun Meteorologi El Tari Kupang)

Penilaian prestasi kinerja adalah suatu proses penilaian yang dilakukan pemimpin Perusahaan secara sistematik berdasarkan pekerjaan yang ditugaskan kepada pegawainya. Stasiun Meteorologi El Tari Kupang merupakan salah satu instansi pemerintahan yang menerapkan penilaian kinerja pegawai untuk tujuan promosi jabatan ataupun pemilihan pegawai berprestasi. Untuk membantu Kepala Stasiun Meteorologi El Tari Kupang dalam proses penilaian kinerja pegawai maka sangat penting dibangun sebuah sistem penilaian kinerja. Penerapan Sistem Pendukung Keputusan (SPK) terkomputerisasi yang mampu membantu proses penilaian kinerja pegawai. Penelitian ini menggunakan metode Analytical Hierarchy Process (AHP) untuk penilaian prestasi kerja pegawai. Sistem yang dibangun berbasis web dan menggunakan kriteria penilaian pegawai meliputi kedisiplinan, prestasi kerja, tanggung jawab dan kompetensi bidang. Hasil analisis yang didapat mampu memberikan urutan rangking terbaik, sehingga menghasilkan kriteria dengan bobot tertinggi yang dapat digunakan sebagai pertimbangan dalam pengambilan keputusan. Hasil pengujian menunjukkan bahwa aplikasi sistem penilaian kinerja pegawai Stasiun Meteorologi El Tari Kupang dengan metode Analytical Hierarchy Proccess dapat berfungsi dengan baik. Hasil perbandingan nilai akhir antara perhitungan sistem dengan perhitungan metode AHP secara manual memiliki nilai yang sama. Hasil perbandingan perangkingan antara sistem dengan data penilaian manual Kantor Stasiun Meteorologi El Tari Kupang mempunyai persamaan urutan perangkingan sebanyak 9 dari 16 pegawai

Comparative epigenomics in distantly related teleost species identifies conserved cis-regulatory nodes active during the vertebrate phylotypic period

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months. After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International).The complex relationship between ontogeny and phylogeny has been the subject of attention and controversy since von Baer's formulations in the 19th century. The classic concept that embryogenesis progresses from clade general features to species-specific characters has often been revisited. It has become accepted that embryos from a clade show maximum morphological similarity at the so-called phylotypic period (i.e., during mid-embryogenesis). According to the hourglass model, body plan conservation would depend on constrained molecular mechanisms operating at this period. More recently, comparative transcriptomic analyses have provided conclusive evidence that such molecular constraints exist. Examining cis-regulatory architecture during the phylotypic period is essential to understand the evolutionary source of body plan stability. Here we compare transcriptomes and key epigenetic marks (H3K4me3 and H3K27ac) from medaka (Oryzias latipes) and zebrafish (Danio rerio), two distantly related teleosts separated by an evolutionary distance of 115-200 Myr. We show that comparison of transcriptome profiles correlates with anatomical similarities and heterochronies observed at the phylotypic stage. Through comparative epigenomics, we uncover a pool of conserved regulatory regions (≈700), which are active during the vertebrate phylotypic period in both species. Moreover, we show that their neighboring genes encode mainly transcription factors with fundamental roles in tissue specification. We postulate that these regulatory regions, active in both teleost genomes, represent key constrained nodes of the gene networks that sustain the vertebrate body plan.The Andalusian government (JA) supported A.F-.M. as scientific manager of the Aquatic Vertebrates Platform at CABD. J.W.C. was supported by a studentship from The Institute of Cancer Research. Spanish and Andalusian government grants BFU2010-14839, CSD2007-00008, and P08-CVI-3488 to J.L.G-.S.; and BFU2011-22916 and P11-CVI-7256 to J.R.M-.M. supported this work.Peer Reviewe

Predictors of functional deterioration in Chinese patients with Psoriatic arthritis: A longitudinal study.

10.1186/1471-2474-15-284BMC Musculoskeletal Disorders15128

Extensive conservation of ancient microsynteny across metazoans due to cis-regulatory constraints

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date; after six months, it is available under a Creative Commons License.-- et al.The order of genes in eukaryotic genomes has generally been assumed to be neutral, since gene order is largely scrambled over evolutionary time. Only a handful of exceptional examples are known, typically involving deeply conserved clusters of tandemly duplicated genes (e.g., Hox genes and histones). Here we report the first systematic survey of microsynteny conservation across metazoans, utilizing 17 genome sequences. We identified nearly 600 pairs of unrelated genes that have remained tightly physically linked in diverse lineages across over 600 million years of evolution. Integrating sequence conservation, gene expression data, gene function, epigenetic marks, and other genomic features, we provide extensive evidence that many conserved ancient linkages involve (1) the coordinated transcription of neighboring genes, or (2) genomic regulatory blocks (GRBs) in which transcriptional enhancers controlling developmental genes are contained within nearby bystander genes. In addition, we generated ChIP-seq data for key histone modifications in zebrafish embryos, which provided further evidence of putative GRBs in embryonic development. Finally, using chromosome conformation capture (3C) assays and stable transgenic experiments, we demonstrate that enhancers within bystander genes drive the expression of genes such as Otx and Islet, critical regulators of central nervous system development across bilaterians. These results suggest that ancient genomic functional associations are far more common than previously thought—involving ∼12% of the ancestral bilaterian genome—and that cis-regulatory constraints are crucial in determining metazoan genome architecture.M.I., M.S.A., S.W.R., and H.B.F. were funded by NIH grant 1R21HG005240-01A1. H.B.F. is an Alfred P. Sloan Fellow and Pew Scholar in the Biomedical Sciences. J.J.T., A.F-M., O.B., E.C-M., and J.L.G-S. were funded by grants BFU2010-14839, CSD2007-00008, and Proyecto de Excelencia CVI-3488.Peer reviewe

Immunolocalization of dually phosphorylated MAPKs in dividing root meristem cells of Vicia faba, Pisum sativum, Lupinus luteus and Lycopersicon esculentum

Key message In plants, phosphorylated MAPKs display constitutive nuclear localization; however, not all studied plant species show co-localization of activated MAPKs to mitotic microtubules. Abstract The mitogen-activated protein kinase (MAPK) signaling pathway is involved not only in the cellular response to biotic and abiotic stress but also in the regulation of cell cycle and plant development. The role of MAPKs in the formation of a mitotic spindle has been widely studied and the MAPK signaling pathway was found to be indispensable for the unperturbed course of cell division. Here we show cellular localization of activated MAPKs (dually phosphorylated at their TXY motifs) in both interphase and mitotic root meristem cells of Lupinus luteus, Pisum sativum, Vicia faba (Fabaceae) and Lycopersicon esculentum (Solanaceae). Nuclear localization of activated MAPKs has been found in all species. Colocalization of these kinases to mitotic microtubules was most evident in L. esculentum, while only about 50 % of mitotic cells in the root meristems of P. sativum and V. faba displayed activated MAPKs localized to microtubules during mitosis. Unexpectedly, no evident immunofluorescence signals at spindle microtubules and phragmoplast were noted in L. luteus. Considering immunocytochemical analyses and studies on the impact of FR180204 (an inhibitor of animal ERK1/2) on mitotic cells, we hypothesize that MAPKs may not play prominent role in the regulation of microtubule dynamics in all plant species

Alternative Splicing Changes Promoted by NOVA2 Upregulation in Endothelial Cells and Relevance for Gastric Cancer

Angiogenesis is crucial for cancer progression. While several anti-angiogenic drugs are in use for cancer treatment, their clinical benefits are unsatisfactory. Thus, a deeper understanding of the mechanisms sustaining cancer vessel growth is fundamental to identify novel biomarkers and therapeutic targets. Alternative splicing (AS) is an essential modifier of human proteome diversity. Nevertheless, AS contribution to tumor vasculature development is poorly known. The Neuro-Oncological Ventral Antigen 2 (NOVA2) is a critical AS regulator of angiogenesis and vascular development. NOVA2 is upregulated in tumor endothelial cells (ECs) of different cancers, thus representing a potential driver of tumor blood vessel aberrancies. Here, we identified novel AS transcripts generated upon NOVA2 upregulation in ECs, suggesting a pervasive role of NOVA2 in vascular biology. In addition, we report that NOVA2 is also upregulated in ECs of gastric cancer (GC), and its expression correlates with poor overall survival of GC patients. Finally, we found that the AS of the Rap Guanine Nucleotide Exchange Factor 6 (RapGEF6), a newly identified NOVA2 target, is altered in GC patients and associated with NOVA2 expression, tumor angiogenesis, and poor patient outcome. Our findings provide a better understanding of GC biology and suggest that AS might be exploited to identify novel biomarkers and therapeutics for anti-angiogenic GC treatments

The role of Cis-Regulatory elements in morphological adaptation to cave environment in Astyanax mexicanus

Trabajo presentado en EMBO Workshop Enhanceropathies: Understanding enhancer function to understand human disease, celebrado en Santander (España) del 06 al 09 de octubre de 2021