Analyses of moisture in polymers and composites

A suitable method for the direct measurement of moisture concentrations after humidity/thermal exposure on state of the art epoxy and polyimide resins and their graphite and glass fiber reinforcements was investigated. Methods for the determination of moisture concentration profiles, moisture diffusion modeling and moisture induced chemical changes were examined. Carefully fabricated, precharacterized epoxy and polyimide neat resins and their AS graphite and S glass reinforced composites were exposed to humid conditions using heavy water (D20), at ambient and elevated temperatures. These specimens were fixtured to theoretically limit the D20 permeation to a unidirectional penetration axis. The analytical techniques evaluated were: (1) laser pyrolysis gas chromatography mass spectrometry; (2) solids probe mass spectrometry; (3) laser pyrolysis conventional infrared spectroscopy; and (4) infrared imaging thermovision. The most reproducible and sensitive technique was solids probe mass spectrometry. The fabricated exposed specimens were analyzed for D20 profiling after humidity/thermal conditioning at three exposure time durations

Sensitive and Precise Quantification of Insulin-Like mRNA Expression in Caenorhabditis elegans

Insulin-like signaling regulates developmental arrest, stress resistance and lifespan in the nematode Caenorhabditis elegans. However, the genome encodes 40 insulin-like peptides, and the regulation and function of individual peptides is largely uncharacterized. We used the nCounter platform to measure mRNA expression of all 40 insulin-like peptides as well as the insulin-like receptor daf-2, its transcriptional effector daf-16, and the daf-16 target gene sod-3. We validated the platform using 53 RNA samples previously characterized by high density oligonucleotide microarray analysis. For this set of genes and the standard nCounter protocol, sensitivity and precision were comparable between the two platforms. We optimized conditions of the nCounter assay by varying the mass of total RNA used for hybridization, thereby increasing sensitivity up to 50-fold and reducing the median coefficient of variation as much as 4-fold. We used deletion mutants to demonstrate specificity of the assay, and we used optimized conditions to assay insulin-like gene expression throughout the C. elegans life cycle. We detected expression for nearly all insulin-like genes and find that they are expressed in a variety of distinct patterns suggesting complexity of regulation and specificity of function. We identified insulin-like genes that are specifically expressed during developmental arrest, larval development, adulthood and embryogenesis. These results demonstrate that the nCounter platform provides a powerful approach to analyzing insulin-like gene expression dynamics, and they suggest hypotheses about the function of individual insulin-like genes

Development of carbon slurry fuels for transportation (hybrid fuels, phase 2)

Slurry fuels of various forms of solids in diesel fuel are developed and evaluated for their relative potential as fuel for diesel engines. Thirteen test fuels with different solids concentrations are formulated using eight different materials. A variety of properties are examined including ash content, sulfur content, particle size distribution, and rheological properties. Attempts are made to determine the effects of these variations on these fuel properties on injection, atomization, and combustion processes. The slurries are also tested in a single cylinder CLR engine in both direct injection and prechamber configurations. The data includes the normal performance parameters as well as heat release rates and emissions. The slurries perform very much like the baseline fuel. The combustion data indicate that a large fraction (90 percent or more) of the solids are burning in the engine. It appears that the prechamber engine configuration is more tolerant of the slurries than the direct injection configuration

Signal Characteristics of Civil GPS Jammers

This paper surveys the signal properties of 18 commercially available GPS jammers based on experimental data. The paper is divided into two distinct tests. The first characterizes the jamming signals, and the second test determines the effective range of 4 of the jammers. The first test uses power spectra from discrete Fourier transforms (DFTs) of the time series data to show that all the jammers employ approximately the same jamming method, i.e. linear frequency modulation of a single tone. The spectra also show that there are significant jammer-to-jammer variations, including between jammers of the same model, and that a given jammer’s signal may vary over time. The first test also includes measurements of signal power within frequency bands centered at the L1 and L2 frequencies, along with the sweep periods and the sweep range at both frequencies. The second test presents measurements of the attenuation of the jamming signal necessary to allow a commercially available GPS receiver to acquire and track signals from a GPS simulator. From the attenuation levels and some assumptions about the antennas used, upper limits on the effective jamming ranges are calculated for 4 of the jammers, with a resulting maximum range of 6–9 km.Aerospace Engineering and Engineering Mechanic

Formation of Cosmic Dust Bunnies

Planetary formation is an efficient process now thought to take place on a relatively short astronomical time scale. Recent observations have shown that the dust surrounding a protostar emits more efficiently at longer wavelengths as the protoplanetary disk evolves, suggesting that the dust particles are coagulating into fluffy aggregates, "much as dust bunnies form under a bed." One poorly understood problem in this coagulation process is the manner in which micron-sized, charged grains form the fractal aggregate structures now thought to be the precursors of protoplanetary disk evolution. This study examines the characteristics of such fractal aggregates formed by the collision of spherical monomers and aggregates where the charge is distributed over the aggregate structure. The aggregates are free to rotate due to collisions and dipole-dipole electrostatic interactions. Comparisons are made for different precursor size distributions and like-charged, oppositelycharged, and neutral grains

LDEF Space Plasma-High Voltage Drainage Experiment post-flight results

The Space Plasma-High Voltage Drainage Experiment (SP-HVDE) was comprised of two identical experimental trays. With one tray located on the leading (ram facing, B10) edge and the other located on the trailing (wake facing, D4) edge of the Long Duration Exposure Facility (LDEF), it was possible to directly compare the effects of ram and wake spacecraft environments on charged dielectric materials. Six arrays of Kapton dielectric samples of 2 mil, 3 mil, and 5 mil thicknesses maintained at +/- 300, +/- 500, and +/- 1000 voltage bias formed the experimental matrix of each tray. In addition, each tray carried two solar cell strings, one biased at +300 volts and the other at -300 volts, to study current leakage from High Voltage Solar Arrays (HVSA). The SP-HVDE provides the first direct, long-term, in-flight measurements of average leakage current through dielectric materials under electric stress. The experiment also yields information on the long term stability of the bulk dielectric properties of such materials. Data and findings of the SP-HVDE are an extension of those from shorter term flight experiments such as the PIX-1 (Plasma Interaction Experiment) and PIX-2 and are therefore valuable in the design and evaluation of long-lived space systems with high voltage systems exposed to the low earth orbital environment. A summary of the SP-HVDE post flight analysis final report delivered to the LDEF Project Office under contract to the National Aeronautics and Space Administration is presented

Mitochondria and neuroprotection in stroke: Cationic arginine-rich peptides (CARPs) as a novel class of mitochondria-targeted neuroprotective therapeutics

Stroke is the second leading cause of death globally and represents a major cause of devastating long-term disability. Despite sustained efforts to develop clinically effective neuroprotective therapies, presently there is no clinically available neuroprotective agent for stroke. As a central mediator of neurodamaging events in stroke, mitochondria are recognised as a critical neuroprotective target, and as such, provide a focus for developing mitochondrial-targeted therapeutics. In recent years, cationic arginine-rich peptides (CARPs) have been identified as a novel class of neuroprotective agent with several demonstrated mechanisms of action, including their ability to target mitochondria and exert positive effects on the organelle. This review provides an overview on neuronal mitochondrial dysfunction in ischaemic stroke pathophysiology and highlights the potential beneficial effects of CARPs on mitochondria in the ischaemic brain following stroke

The Spectral Variability of Cygnus X-1 at MeV Energies

In previous work, we have used data from the first three years of the CGRO mission to assemble a broad-band $\gamma$-ray spectrum of the galactic black hole candidate Cygnus X-1. Contemporaneous data from the COMPTEL, OSSE and BATSE experiments on CGRO were selected on the basis of the hard X-ray flux (45--140 keV) as measured by BATSE. This provided a spectrum of Cygnus X-1 in its canonical low X-ray state (as measured at energies below 10 keV), covering the energy range from 50 keV to 5 MeV. Here we report on a comparison of this spectrum to a COMPTEL-OSSE spectrum collected during a high X-ray state of Cygnus X-1 (May, 1996). These data provide evidence for significant spectral variability at energies above 1 MeV. In particular, whereas the hard X-ray flux {\it decreases} during the high X-ray state, the flux at energies above 1 MeV {\it increases}, resulting in a significantly harder high energy spectrum. This behavior is consistent with the general picture of galactic black hole candidates having two distinct spectral forms at soft $\gamma$-ray energies. These data extend this picture, for the first time, to energies above 1 MeV.Comment: 5 pages, 4 figures, to be published in AIP Conf. Proc., "The Fifth Compton Symposium