8 research outputs found
Self similar Barkhausen noise in magnetic domain wall motion
A model for domain wall motion in ferromagnets is analyzed. Long-range
magnetic dipolar interactions are shown to give rise to self-similar dynamics
when the external magnetic field is increased adiabatically. The power spectrum
of the resultant Barkhausen noise is of the form , where
can be estimated from the critical exponents for interface
depinning in random media.Comment: 7 pages, RevTex. To appear in Phys. Rev. Let
Dynamics of a ferromagnetic domain wall and the Barkhausen effect
We derive an equation of motion for the the dynamics of a ferromagnetic
domain wall driven by an external magnetic field through a disordered medium
and we study the associated depinning transition. The long-range dipolar
interactions set the upper critical dimension to be , so we suggest that
mean-field exponents describe the Barkhausen effect for three-dimensional soft
ferromagnetic materials. We analyze the scaling of the Barkhausen jumps as a
function of the field driving rate and the intensity of the demagnetizing
field, and find results in quantitative agreement with experiments on
crystalline and amorphous soft ferromagnetic alloys.Comment: 4 RevTex pages, 3 ps figures embedde
Dynamics of a ferromagnetic domain wall: avalanches, depinning transition and the Barkhausen effect
We study the dynamics of a ferromagnetic domain wall driven by an external
magnetic field through a disordered medium. The avalanche-like motion of the
domain walls between pinned configurations produces a noise known as the
Barkhausen effect. We discuss experimental results on soft ferromagnetic
materials, with reference to the domain structure and the sample geometry, and
report Barkhausen noise measurements on FeCoB amorphous
alloy. We construct an equation of motion for a flexible domain wall, which
displays a depinning transition as the field is increased. The long-range
dipolar interactions are shown to set the upper critical dimension to ,
which implies that mean-field exponents (with possible logarithmic correction)
are expected to describe the Barkhausen effect. We introduce a mean-field
infinite-range model and show that it is equivalent to a previously introduced
single-degree-of-freedom model, known to reproduce several experimental
results. We numerically simulate the equation in , confirming the
theoretical predictions. We compute the avalanche distributions as a function
of the field driving rate and the intensity of the demagnetizing field. The
scaling exponents change linearly with the driving rate, while the cutoff of
the distribution is determined by the demagnetizing field, in remarkable
agreement with experiments.Comment: 17 RevTeX pages, 19 embedded ps figures + 1 extra figure, submitted
to Phys. Rev.
Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor
Despite decades of research in the epidermal growth factor receptor (EGFR) signalling field, and many targeted anti-cancer drugs that have been tested clinically, the success rate for these agents in the clinic is low, particularly in terms of the improvement of overall survival. Intratumoral heterogeneity is proposed as a major mechanism underlying treatment failure of these molecule-targeted agents. Here we highlight the application of fluorescence lifetime microscopy (FLIM)-based biosensing to demonstrate intratumoral heterogeneity of EGFR activity. For sensing EGFR activity in cells, we used a genetically encoded CrkII-based biosensor which undergoes conformational changes upon tyrosine-221 phosphorylation by EGFR. We transfected this biosensor into EGFR-positive tumour cells using targeted lipopolyplexes bearing EGFR-binding peptides at their surfaces. In a murine model of basal-like breast cancer, we demonstrated a significant degree of intratumoral heterogeneity in EGFR activity, as well as the pharmacodynamic effect of a radionuclide-labeled EGFR inhibitor in situ. Furthermore, a significant correlation between high EGFR activity in tumour cells and macrophage-tumour cell proximity was found to in part account for the intratumoral heterogeneity in EGFR activity observed. The same effect of macrophage infiltrate on EGFR activation was also seen in a colorectal cancer xenograft. In contrast, a non-small cell lung cancer xenograft expressing a constitutively active EGFR conformational mutant exhibited macrophage proximity-independent EGFR activity. Our study validates the use of this methodology to monitor therapeutic response in terms of EGFR activity. In addition, we found iNOS gene induction in macrophages that are cultured in tumour cell-conditioned media as well as an iNOS activity-dependent increase in EGFR activity in tumour cells. These findings point towards an immune microenvironment-mediated regulation that gives rise to the observed intratumoral heterogeneity of EGFR signalling activity in tumour cells in vivo