69 research outputs found
The Molecular Identification of Organic Compounds in the Atmosphere: State of the Art and Challenges
STUDIES IN THE PHYSIOLOGY OF BLOOD VESSELS IN MAN. APPARATUS AND METHODS. II. A METHOD FOR THE DETERMINATION OF THE VOLUME OF THE SOFT TISSUE ABOUT THE TERMINAL PHALANX OF THE HUMAN FINGER 1
Nonhemocyte sources of selected lysosomal enzymes in Biomphalaria glabrata, B. tenagophila and B. straminea (Mollusca: Pulmonata)
The specific activities of acid phosphatase, alkaline phosphatase, β-glucuronidase, lysozymes, glutamate-oxalacetate transaminase and glutamate-pyruvate transaminate were determined in the head-foot and digestive gland of Brazilian Biomphalaria glabrata (Touros), B. tenagophila (Caçapava) and B. straminea (Monsenhor Gil). All six enzymes were detected inthe 3000g supernatant. Both cytoplasmic enzymes, glutamate-oxalacetate and glutamate-pyruvate transaminase exhibited the highest specific activities. In the case of the four hydrolytic enzymes assayed, β-glucuronidase exhibited the highest specific activity while lysozyme showed the lowest activity. All six enzymes are thought to be produced by cells within the head-foot and digestive gland of B. glabrata, B. tenagophila and B. straminea.<br>Foram determinadas, na massa cefalopedal e na glândula digestiva de Biomphalaria glabrata de Touros (Rio Grande do Norte) B. tenagophila de Cacapava (Sao Paulo) e B. straminea de Monsenhor Gil (PiauÃ), as atividades especÃficas das seguintes enzimas: fosfatase acida, fosfatase alcalina, beta-glucuronidase, lisozima, transaminase glutâmico-oxalacetica e transaminase glutâmico-piruvica. As seis enzimas referidas foram detectadas no sobrenadante a 3000g. Ambas as enzimas citoplasmaticas - transaminases glutamico-oxalacetica e glutamico-piruvica - mostraram as atividades especÃficas mais altas. No caso das quatro enzimas hidrolÃticas, a beta-glucuronidase revelou a mais alta atividade especÃfica, enquanto a lisozima revelou a mais baixa. E admitido que todas as seis enzimas sao produzidas por celulas presentes na massa cefalopedal e na glândula digestiva das tres especies de moluscos examinadas
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