Long-Term Results and Prognostic Factors of Gastric Cancer Patients with Microscopic Peritoneal Carcinomatosis

BACKGROUND: Clinical significance of microscopic peritoneal carcinomatosis remained unclear. The aim of this study was to evaluate the prognostic value of microscopic peritoneal carcinomatosis in gastric cancer. METHODS: From 1996 to 2007, 4426 patients underwent gastrectomy for gastric cancer at Fudan University Shanghai Cancer Center. The clinical and pathological data were reviewed to identify patients with microscopic peritoneal carcinomatosis (group 1). The clinicopathological features and prognosis were examined. Additionally, 242 stage-matched gastric cancer patients without microscopic peritoneal carcinomatosis (group 2) and 118 with macroscopic peritoneal carcinomatosis (group 3) were selected as control groups. RESULTS: Microscopic peritoneal carcinomatosis was found in 121 patients. There were 85 males and 36 females (2.36:1). There was a higher incidence rate of large size tumor (≥5 cm) (P = 0.045), Borrmann IV (P = 0.000), and serosal invasion (P = 0.000) in gastric cancer with microscopic peritoneal carcinomatosis compared with the control group. The 5-year survival rate of gastric cancer with microscopic peritoneal carcinomatosis was 24%, significantly poorer than that of the stage-matched control group but better than that of patients with macroscopic peritoneal carcinomatosis. The independent prognostic factors identified included pathological stage and operative curability. CONCLUSIONS: The presence of microscopic peritoneal carcinomatosis was associated with worse prognosis for gastric cancer, but curative surgery showed potential to improve prognosis

Malformation rate of the developing f ish embryo of Plectorhinchus cinctus to indicate marine environment qual ity

[摘要]：以养殖场培育的花尾胡椒鲷受精卵为实验材料测试了厦门西海域5 个站位的表层水水样对花尾胡椒 鲷受精卵的胚胎发育毒性,并运用GC2MS 手段分析了水样中的美国EPA 优控的16 种PAHs 组分的含量。结 果表明:3 号站位(厦门市工业和生活污水主要排放口的外侧) 和4 号站位(厦门西港的港区) 的水样对鱼卵的胚 胎发育的毒性最强;8 号站位(位于主航道并濒临嵩屿电厂) 的水样对鱼卵胚胎发育的毒性次之,1 号站位(湾 口) 和5 号站位(内港) 的水样对鱼卵的胚胎发育的毒性较小。水样中的PAHs 含量分析结果表明,仅用水样中 的PAHs 含量不能全面地反映水样对鱼卵胚胎发育的毒性。最后,对本实验方法应用于海洋环境生物监测的有 效性进行了探讨。[Abstract]:Cultured zygotes of Plectorhi nchus ci nct us were done to make test the toxicity of surface water samples in Western Xia2 men Sea Area. The results showed that the water samples at Station 3 (the outlet of industrial and domestic sewage of Xiamen City) and Station 4 (the major port area of Xiamen Island) were the most toxic , it would be revealed that the industrial and domestic sewage and port pollution were the major sources of pollutants. Station 8 (in the sea - route and nearby a big power plant) was less toxic. The toxicity at station 1 (the outlet of Western Xiamen Bay) and Station 5 (the inner port area) were the least . The results showed that it was useful to test the toxicity of water samples by the method. Simultaneously sixteen components of EPA priority PAHs in these samples were also measured by GC/ MS (SIM mode) . The sequence of the toxicity to zygotes and the sequence of the concentrations of total PAHs were not the same , since the factors influencing the toxicity to zygotes were complicated , it would be also revealed that the method was useful to indicate the total marine environment quality.国家自然科学基金资助项目(A20077023 ,C40106012

Dysregulated Expression of Neuregulin-1 by Cortical Pyramidal Neurons Disrupts Synaptic Plasticity

SummaryNeuregulin-1 (NRG1) gene variants are associated with increased genetic risk for schizophrenia. It is unclear whether risk haplotypes cause elevated or decreased expression of NRG1 in the brains of schizophrenia patients, given that both findings have been reported from autopsy studies. To study NRG1 functions in vivo, we generated mouse mutants with reduced and elevated NRG1 levels and analyzed the impact on cortical functions. Loss of NRG1 from cortical projection neurons resulted in increased inhibitory neurotransmission, reduced synaptic plasticity, and hypoactivity. Neuronal overexpression of cysteine-rich domain (CRD)-NRG1, the major brain isoform, caused unbalanced excitatory-inhibitory neurotransmission, reduced synaptic plasticity, abnormal spine growth, altered steady-state levels of synaptic plasticity-related proteins, and impaired sensorimotor gating. We conclude that an “optimal” level of NRG1 signaling balances excitatory and inhibitory neurotransmission in the cortex. Our data provide a potential pathomechanism for impaired synaptic plasticity and suggest that human NRG1 risk haplotypes exert a gain-of-function effect

Potent Neutralization of Influenza A Virus by a Single-Domain Antibody Blocking M2 Ion Channel Protein

Influenza A virus poses serious health threat to humans. Neutralizing antibodies against the highly conserved M2 ion channel is thought to offer broad protection against influenza A viruses. Here, we screened synthetic Camel single-domain antibody (VHH) libraries against native M2 ion channel protein. One of the isolated VHHs, M2-7A, specifically bound to M2-expressed cell membrane as well as influenza A virion, inhibited replication of both amantadine-sensitive and resistant influenza A viruses in vitro, and protected mice from a lethal influenza virus challenge. Moreover, M2-7A showed blocking activity for proton influx through M2 ion channel. These pieces of evidence collectively demonstrate for the first time that a neutralizing antibody against M2 with broad specificity is achievable, and M2-7A may have potential for cross protection against a number of variants and subtypes of influenza A viruses

Probing the Behaviors of Gold Nanorods in Metastatic Breast Cancer Cells Based on UV-vis-NIR Absorption Spectroscopy

In this work, behaviors of positively-charged AuNRs in a highly metastatic tumor cell line MDA-MB-231 are examined based on UV-vis-NIR absorption spectroscopy in combination with inductively coupled plasma mass spectrometry (ICP-MS), transmission electron microscopy (TEM) and dark-field microscopic observation. It is found that characteristic surface plasmon resonance (SPR) peaks of AuNRs can be detected using spectroscopic method within living cells that have taken up AuNRs. The peak area of transverse SPR band is shown to be proportionally related to the amount of AuNRs in the cells determined with ICP-MS, which suggests a facile and real time quantification method for AuNRs in living cells. The shape of longitudinal SPR band in UV-vis-NIR spectrum reflects the aggregation state of AuNRs in the cells during the incubation period, which is proved by TEM and microscopic observations. Experimental results reveal that AuNRs are internalized by the cells rapidly; the accumulation, distribution and aggregation of AuNRs in the cells compartments are time and dose dependent. The established spectroscopic analysis method can not only monitor the behaviors of AuNRs in living cells but may also be helpful in choosing the optimum laser stimulation wavelength for anti-tumor thermotherapy

Dynamics of Local Search Trajectory in Traveling Salesman Problem

This paper investigates dynamics of a local search trajectory generated by running the Or-opt heuristic on the traveling salesman problem. This study evaluates the dynamics of the local search heuristic by estimating the correlation dimension for the search trajectory, and finds that the local heuristic search process exhibits the transition from high-dimensional stochastic to low-dimensional chaotic behavior. The detection of dynamical complexity for a heuristic search process has both practical as well as theoretical relevance. The revealed dynamics may cast new light on design and analysis of heuristics and result in the potential for improved search process.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/45818/1/10732_2005_Article_3604.pd

A heterozygous moth genome provides insights into herbivory and detoxification

How an insect evolves to become a successful herbivore is of profound biological and practical importance. Herbivores are often adapted to feed on a specific group of evolutionarily and biochemically related host plants1, but the genetic and molecular bases for adaptation to plant defense compounds remain poorly understood2. We report the first whole-genome sequence of a basal lepidopteran species, Plutella xylostella, which contains 18,071 protein-coding and 1,412 unique genes with an expansion of gene families associated with perception and the detoxification of plant defense compounds. A recent expansion of retrotransposons near detoxification-related genes and a wider system used in the metabolism of plant defense compounds are shown to also be involved in the development of insecticide resistance. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore and offers wider insights into insect adaptation to plant feeding, as well as opening avenues for more sustainable pest management.Minsheng You … Simon W Baxter … et al