Inhibition of the activity of pro-inflammatory secretory phospholipase A2 by acute phase proteins

Pro-Inflammatory non-pancreatic phospholipase A2 (sPLA2) is markedly over-expressed in acute systemic and chronic local inflammatory processes. Since in acute phase reaction sPLA2 is often over-expressed simultaneously with acute phase proteins (APP), it is important to determine whether APP interacts with sPLA2. We tested ten APPs for interaction with sPLA2 using as a substrate multilamellar Hposomes composed either of PC:Lyso PC or PE:Lyso PE. Using PC:Lyso PC substrate, CRP, lactoferrin and SAP were found to inhibit sPLA2 activity with an IC50 of 25 μg/ml, 7.5 μg/ml and 50 μg/ml, respectively, corresponding to 0.21 μM, 0.1 μM and 0.21 μM respectively. Using PE:Lyso PE substrate only SAP was inhibitory, with an IC50 of 10 μg/ml (0.04 μM). Phosphorylcholine abolished the inhibitory activity of CRP but not of SAP or lactoferrin. Addition of phosphorylethanolamine or of excess calcium had no effect on the inhibitory activity of APP. Limulin, lysozyme, transferrin, β2-microglobulin, α2-macroglobulin, human and bovine albumins had no effect on sPLA2 activity. Therefore neither the structure of pentraxins, or ironbinding, bacteriostatic property or amyloidogenic property preclude whether APP modulates sPLA2 activity. Inhibition of pro-inflammatory sPLA2 by APP may be one of the protective mechanisms of the acute phase reaction

Semiclassical Strings in Electric and Magnetic Fields Deformed AdS5×S5AdS_5 \times S^5 Spacetimes

We first apply the transformation of mixing azimuthal and internal coordinate or mixing time and internal coordinate to the 11D M-theory with a stack N M2-branes to find the spacetime of a stack of N D2-branes with magnetic or electric flux in 10 D IIA string theory, after the Kaluza-Klein reduction. We then perform the T duality to the spacetime to find the background of a stack of N D3-branes with magnetic or electric flux. In the near-horizon limit the background becomes the magnetic or electric field deformed $AdS_5 \times S^5$. We adopt an ansatz to find the classical string solution which is rotating in the deformed $S^5$ with three angular momenta in the three rotation planes. The relations between the classical string energy and its angular momenta are found and results show that the external magnetic and electric fluxes will increase the string energy. Therefore, from the AdS/CFT point of view, the corrections of the anomalous dimensions of operators in the dual SYM theory will be positive. We also investigate the small fluctuations in these solutions and discuss the effects of magnetic and electric fields on the stability of these classical rotating string solutions. Finally, we find the possible solutions of string pulsating on the deformed spacetimes and show that the corrections to the anomalous dimensions of operators in the dual SYM theory are non-negative.Comment: Latex 18 pages, correct sec. 3.

Recherches sur les propriétés antibiotiques des larves de Nématodes parasites

Stefanski W., Przyjalkowski Z. Recherches sur les propriétés antibiotiques des larves de Nématodes parasites. In: Bulletin de l'Académie Vétérinaire de France tome 112 n°8, 1959. pp. 495-498

L’influence de certaines Bactéries sur l’établissement de Trichines dans le tube digestif de la souris

Stefanski W., Przyjalkowski Z. L’influence de certaines bactéries sur l’établissement de Trichines dans le tube digestif de la Souris. In: Bulletin de l'Académie Vétérinaire de France tome 117 n°3, 1964. pp. 131-135

Tenidap sodium inhibits secretory non-pancreatic phospholipase A2 synthesis by foetal rat calvarial osteoblasts

Tenidap (TD) was initially defined as a dual inhibitor of cyclooxygenase and lipoxygenase. This study was designed to assess its inhibitory activity against proinflammatory phospholipase A2. This study shows that TD inhibits the synthesis of pro-inflammatory secretory non-pancreatic phospholipase A2 (sPLA2). Concentrations as low as 0.25 μg/ml (0.725 μM) reduced the release of sPLA2 by 40% from foetal rat calvarial osteoblasts stimulated with IL-1β and TNFα, whereas a concentration of 2.5 μg/ml (7.25 μM) reduced the release by over 80%. TD also markedly reduced the release of sPLA2 from unstimulated cells. There was no direct inhibition of sPLA2 enzymatic activity by TD in vitro. Northern blot analysis showed that TD did not affect the sPLA2 mRNA levels; however, immunoblotting showed a dose-dependent reduction in sPLA2 enzyme. These results, together with a marked reduction in sPLA2 enzymatic activity, suggest that TD inhibits sPLA2 synthesis at the post-transcriptional level. Therefore TD seems to inhibit the arachidonic acid cascade proximally to cyclooxygenase and lipoxygenase and its anti-inflammatory activity may be related at least in part to the inhibition of sPLA2 synthesis

Free field realization of the exceptional current superalgebra \hat{D(2,1;\a)}_k

The free-field representations of the D(2,1;\a) current superalgebra and the corresponding energy-momentum tensor are constructed. The related screening currents of the first kind are also presented.Comment: Latex file, 10 page

Zero-Bias Conductance Through Side-Coupled Double Quantum Dots

Low temperature zero-bias conductance through two side-coupled quantum dots is investigated using Wilson's numerical renormalization group technique. A low-temperature phase diagram is computed. Near the particle-hole symmetric point localized electrons form a spin-singlet associated with weak conductance. For weak inter-dot coupling we find enhanced conductance due to the two-stage Kondo effect when two electrons occupy quantum dots. When quantum dots are populated with a single electron, the system enters Kondo regime with enhanced conductance. Analytical expressions for the width of the Kondo regime and the Kondo temperature in this regime are given.Comment: to be published in the Proceedings of the NATO Advanced Research Workshop on "Electron Correlations in New Materials and Nanosystems" held in Yalta, Ukraine, 19 - 23 September 2005 (NATO Science Series II, Springer 2006

Bohr-Sommerfeld Quantization of Periodic Orbits

We show, that the canonical invariant part of $\hbar$ corrections to the Gutzwiller trace formula and the Gutzwiller-Voros spectral determinant can be computed by the Bohr-Sommerfeld quantization rules, which usually apply for integrable systems. We argue that the information content of the classical action and stability can be used more effectively than in the usual treatment. We demonstrate the improvement of precision on the example of the three disk scattering system.Comment: revte

Introduction to COFFE: The Next-Generation HPCMP CREATE-AV CFD Solver

HPCMP CREATE-AV Conservative Field Finite Element (COFFE) is a modular, extensible, robust numerical solver for the Navier-Stokes equations that invokes modularity and extensibility from its first principles. COFFE implores a flexible, class-based hierarchy that provides a modular approach consisting of discretization, physics, parallelization, and linear algebra components. These components are developed with modern software engineering principles to ensure ease of uptake from a user's or developer's perspective. The Streamwise Upwind/Petrov-Galerkin (SU/PG) method is utilized to discretize the compressible Reynolds-Averaged Navier-Stokes (RANS) equations tightly coupled with a variety of turbulence models. The mathematics and the philosophy of the methodology that makes up COFFE are presented

Circular and Folded Multi-Spin Strings in Spin Chain Sigma Models

From the SU(2) spin chain sigma model at the one-loop and two-loop orders we recover the classical circular string solution with two S^5 spins (J_1, J_2) in the AdS_5 x S^5 string theory. In the SL(2) sector of the one-loop spin chain sigma model we explicitly construct a solution which corresponds to the folded string solution with one AdS_5 spin S and one S^5 spin J. In the one-loop general sigma model we demonstrate that there exists a solution which reproduces the energy of the circular constant-radii string solution with three spins (S_1, S_2, J).Comment: 16 pages, LaTeX, no figure