Biosynthesis of Cholinesterase-inhibitory Substances from Non-inhibitory Dialkyl Thiophosphates by Plant Tissues

Aqueous solutions of the potassium salts of diethyl thiophosphate and diethyl dithiophosphate were applied to intact leaves of the following 10 plant species: alfalfa (Medicago saliva L.), carrot (Daucus carota L.), strawberry (Fragaria chiloensis, Duchesne), American elderberry (Sambucus canadensis L.), velvetleaf (Abutilon theophrasti Medic.), dandelion (Taraxacum officinale Weber), soybean (Glycine max, Merr.), lima bean (Phaseolus limensis Macf.), catnip (Nepeta cataria L.), and Virginia creeper (Hedera quinquefolia L.). After 24 to 72 hours, aqueous homogenates of all species, excepting carrot, inhibited the action of human plasma cholinesterase in vitro. Neither the plant homogenates nor the thiophosphate salts either separately or mixed brought about inhibition. It is postulated that dialkyl thio- or dithiophosphates are incorporated by intact living plant tissue into cholinesterase-inhibitory esters

A Preliminary Survey of Some Midwestern Plants for Substances Inhibiting Human Plasma Cholinesterase in Vitro

Substances which inhibit the action of human plasma cholinesterase in vitro have been found in aqueous extracts of various members of the plant family Solanaceae. A preliminary survey of some weedy and cultivated midwestern representatives of this family revealed eleven active species in six genera: Datura, Lycopersicon, Nicotiana, Petunia, Physalis, and Solanum. A member each of the genera Capsicum and Lycium were inactive. Inhibitory substances were not found in thirty-one species representing nineteen plant families other than Solanaceae

Emotions in business-to-business service relationships

Emotion in business-to-business service relationships regarding cargo services is explored. The service relationship is characterised by mutual trust and cooperation. Contact is mainly via telephone or e-mail with some face-to-face interactions and participants providing a complex, multi-skilled seamless service. Experience rather than training plays a vital role with long-term service relationships built up and maintained. Emotional sensitivity is acquired partly by experience and a repeat customer base but mainly through a genuine desire to help and get to know others. In contrast to the view of emotional labour bringing managerial control or adverse affects to service staff, the emotion engendered by this work is authentic expression bringing personal satisfaction

Confronting the digital:Doing ethnography in modern organizational settings

Digital technologies pervade modern life. As a result, organizational ethnographers must contend with informants interacting in face-to-face and digitally mediated encounters (e.g., through email, Facebook Messenger, and Skype). This overlap of informants’ digital and physical interactions challenges ethnographers’ ability to demonstrate authenticity and multivocality in their accounts of contemporary organizing. Drawing on recent theorizing about the nature of digital artifacts and two cases of ethnographic fieldwork, we argue that digital artifacts afford ethnographers different modes of being co-present with research participants: digital as archive and digital as process. We offer guidelines to researchers on how to deploy these modes of co-presence in order to improve authenticity and multivocality in ethnographic studies of modern organizations. We also explore the implications for methodological concerns such as ethics, analytical choice, and reflexivity

Communitarian perspectives on social enterprise

Concepts of social enterprise have been debated repeatedly, and continue to cause confusion. In this paper, a meta-theoretical framework is developed through discussion of individualist and communitarian philosophy. Philosophers from both traditions build social theories that emphasise either consensus (a unitarist outlook) or diversity (a pluralist outlook). The various discourses in corporate governance reflect these assumptions and create four distinct approaches that impact on the relationship between capital and labour. In rejecting the traditional discourse of private enterprise, social enterprises have adopted other approaches to tackle social exclusion, each derived from different underlying beliefs about the purpose of enterprise and the nature of governance. The theoretical framework offers a way to understand the diversity found within the sector, including the newly constituted Community Interest Company (CIC).</p

Understanding Video Game Developers as an Occupational Community

The video game industry has rapidly expanded over the last four decades; yet there is limited research about the workers who make video games. In examining these workers, this article responds to calls for renewed attention to the role of the occupation in understanding project-based workers in boundaryless careers. Specifically, this article uses secondary analysis of online sources to demonstrate that video game developers can be understood as a unique social group called an occupational community (OC). Once this classification has been made, the concept of OC can be used in future research to understand video game workers in terms of identity formation, competency development, career advancement and support, collective action, as well as adherence to and deviance from organizational and industry norms

Abi1 gene silencing by short hairpin RNA impairs Bcr-Abl-induced cell adhesion and migration in vitro and leukemogenesis in vivo

Abl interactor (Abi) 1 was first identified as the downstream target of Abl tyrosine kinases and was found to be dysregulated in leukemic cells expressing oncogenic Bcr-Abl and v-Abl. Although the accumulating evidence supports a role of Abi1 in actin cytoskeleton remodeling and growth factor/receptor signaling, it is not clear how it contributes to Bcr-Abl-induced leukemogenesis. We show here that Abi1 gene silencing by short hairpin RNA attenuated the Bcr-Abl-induced abnormal actin remodeling, membrane-type 1 metalloproteinase clustering and inhibited cell adhesion and migration on fibronectin-coated surfaces. Although the knock down of Abi1 expression did not affect growth factor-independent growth of Bcr-Abl-transformed Ba/F3 cells in vitro, it impeded competitive expansion of these cells in non obese diabetic (NOD)/ severe combined immuno-deficiency (SCID) mice. Remarkably, the knock down of Abi1 expression in Bcr-Abl-transformed Ba/F3 cells impaired the leukemogenic potential of these cells in NOD/SCID mice. Abi1 contributes to Bcr-Abl-induced leukemogenesis in part through Src family kinases, as the knock down of Abi1 expression attenuates Bcr-Abl-stimulated activation of Lyn. Together, these data provide for the first time the direct evidence that supports a critical role of Abi1 pathway in the pathogenesis of Bcr-Abl-induced leukemia

What Was I Thinking? Eye-Tracking Experiments Underscore the Bias that Architecture Exerts on Nuclear Grading in Prostate Cancer

We previously reported that nuclear grade assignment of prostate carcinomas is subject to a cognitive bias induced by the tumor architecture. Here, we asked whether this bias is mediated by the non-conscious selection of nuclei that “match the expectation” induced by the inadvertent glance at the tumor architecture. 20 pathologists were asked to grade nuclei in high power fields of 20 prostate carcinomas displayed on a computer screen. Unknown to the pathologists, each carcinoma was shown twice, once before a background of a low grade, tubule-rich carcinoma and once before the background of a high grade, solid carcinoma. Eye tracking allowed to identify which nuclei the pathologists fixated during the 8 second projection period. For all 20 pathologists, nuclear grade assignment was significantly biased by tumor architecture. Pathologists tended to fixate on bigger, darker, and more irregular nuclei when those were projected before kigh grade, solid carcinomas than before low grade, tubule-rich carcinomas (and vice versa). However, the morphometric differences of the selected nuclei accounted for only 11% of the architecture-induced bias, suggesting that it can only to a small part be explained by the unconscious fixation on nuclei that “match the expectation”. In conclusion, selection of « matching nuclei » represents an unconscious effort to vindicate the gravitation of nuclear grades towards the tumor architecture

Embracing the otherness of others: an approach for teacher educators to assuage social and political tribalism

We live in a corrosively polarized climate where sharply divided beliefs about globalization and populism magnify existing biases. This problem is exacerbated by the fact that those who want to educate the next generation – teacher candidates – have been schooled in systems that focus on obedience, conformity, and docility. This breadcrumbs approach to education bears the danger of reproducing the status quo rather than changing it. This chapter explores the promises, perils, and provocations of curriculum regarding how we think about diversity and delineates a learning experience for pre-service teachers that allows them to identify, approach, and become the Other. The lived experience illuminates the cultural and experiential origins of their beliefs while reconciling the uncomfortable notion that bias is part of the human condition. In times where sharpened divisions intensify social and political tribalism it is essential to understand that it is the way one acts upon one’s biases that determines what kind of world one creates