QTL analysis and genomic selection using RADseq derived markers in Sitka spruce: the potential utility of within family data

Sitka spruce (Picea sitchensis (Bong.) Carr) is the most common commercial plantation species in Britain and a breeding programme based on traditional lines has been in operation since the early 1960s. Rotation lengths of 40-years have led breeders to adopt a process of indirect selection at younger ages based on traits well correlated with final selection, but still the generation interval is unlikely to reduce much below twenty years. Recent successful developments with genomic selection in animal breeding have led tree breeders to consider the application of this technology. In this study a RAD sequence assay was developed as a means of investigating the potential of molecular breeding in a non-model species. DNA was extracted from nearly 500 clonally replicated trees growing in a single full-sibling family at one site in Britain. The technique proved successful in identifying 132 QTLs for 5-year bud-burst and 2 QTLs for 6-year height. In addition, the accuracy of predicting phenotypes by genomic selection was strikingly high at 0.62 and 0.59 respectively. Sensitivity analysis with 200 offspring found only a slight fall in correlation values (0.54 and 0.38) although when the training population reduced to 50 offspring predictive values fell further (0.33 and 0.25). This proved an encouraging first investigation into the potential use of genomic selection in the breeding of Sitka spruce. The authors investigate how problems associated with effective population size and linkage disequilibrium can be avoided and suggest a practical way of incorporating genomic selection into a dynamic breeding programme

Thrombospondins in the heart: potential functions in cardiac remodeling

Cardiac remodeling after myocardial injury involves inflammation, angiogenesis, left ventricular hypertrophy and matrix remodeling. Thrombospondins (TSPs) belong to the group of matricellular proteins, which are non-structural extracellular matrix proteins that modulate cell–matrix interactions and cell function in injured tissues or tumors. They interact with different matrix and membrane-bound proteins due to their diverse functional domains. That the expression of TSPs strongly increases during cardiac stress or injury indicates an important role for them during cardiac remodeling. Recently, the protective properties of TSP expression against heart failure have been acknowledged. The current review will focus on the biological role of TSPs in the ischemic and hypertensive heart, and will describe the functional consequences of TSP polymorphisms in cardiac disease

MCM-41 as a support for small platinum particles: a catalyst for low-temperature carbon monoxide oxidation

MCM-41 is loaded with platinum via different pathways, resulting in highly active oxidation catalysts

The John Williams Index of Palaeopalynology

The John Williams Index of Palaeopalynology (JWIP) is the result of the lifetime's work of Dr John E. Williams. Housed at the Department of Palaeontology of The Natural History Museum (NHM) in London, the JWIP is publically available and provides probably the most comprehensive fully cross-referenced catalogue on palaeopalynology in the world. It has 23,350 references to fossil palynomorph genera or species as of February 2012. Since its inception in 1971, every publication in the collection referring to a fossil palynomorph genus or species has been critiqued by John E. Williams. Each item is given an accession number and appropriately referenced within the JWIP using index cards which are sorted alphabetically. Once added to the main reference subindex, further entries are completed for four themed subindexes. The first three of these are sets of cards on the three major palynomorph groups (acritarchs/dinoflagellate cysts, chitinozoa and pollen/spores), 26 stratigraphical intervals and 17 geographical areas. The fourth themed subindex is where each palynomorph taxon has a card (or cards) listing all the records of that species in the literature within six categories (acritarchs, dinoflagellate cysts, chitinozoa, fungal spores, pollen/spores and miscellaneous). Due to the sustained and meticulous recording of data since 1971, users can therefore search the database by major palynomorph group, species, age and/or geographical region. The comprehensive and cross-referenced nature of the JWIP means that researchers can readily identify key publications on, for example, specific palynomorph types over a particular interval in a prescribed area. The JWIP is currently entirely analogue, but the NHM is currently evaluating potential strategies for digitisation