How expertise and language familiarity influence perception of speech of people with Parkinson’s disease

Parkinson’s disease (PD) is a progressive neurological disorder characterized by several motor and non-motor manifestations. PD frequently leads to hypokinetic dysarthria, which affects speech production and often has a detrimental impact on everyday communication. Among the typical manifestations of hypokinetic dysarthria, speech and language therapists (SLTs) identify prosody as the most affected cluster of speech characteristics. However, less is known about how untrained listeners perceive PD speech and how affected prosody influences their assessments of speech. This study explores the perception of sentence type intonation and healthiness of PD speech by listeners with different levels of familiarity with speech disorders in Dutch. We investigated assessments and classification accuracy differences between Dutch-speaking SLTs (n = 18) and Dutch/non-Dutch speaking untrained listeners (n = 27 and n = 124, respectively). We collected speech data from 30 Dutch speakers diagnosed with PD and 30 Dutch healthy controls. The stimuli set consisted of short phrases from spontaneous and read speech and of phrases produced with different sentence type intonation. Listeners participated in an online experiment targeting classification of sentence type intonation and perceived healthiness of speech. Results indicate that both familiarity with speech disorders and with speakers’ language are significant and have different effects depending on the task type, as different listener groups demonstrate different classification accuracy. There is evidence that untrained Dutch listeners classify PD speech as unhealthy more accurately than both trained Dutch and untrained non-Dutch listeners, while trained Dutch listeners outperform the other two groups in sentence type classification

Magnetoresistance of a semiconducting magnetic wire with domain wall

We investigate theoretically the influence of the spin-orbit interaction of Rashba type on the magnetoresistance of a semiconducting ferromagnetic nanostructure with a laterally constrained domain wall. The domain wall is assumed sharp (on the scale of the Fermi wave length of the charge carriers). It is shown that the magnetoresistance in such a case can be considerably large, which is in a qualitative agreement with recent experimental observations. It is also shown that spin-orbit interaction may result in an increase of the magnetoresistance. The role of localization corrections is also briefly discussed.Comment: 5 pages, 2 figure

HAM-5 functions as a MAP kinase scaffold during cell fusion in Neurospora crassa.

Cell fusion in genetically identical Neurospora crassa germlings and in hyphae is a highly regulated process involving the activation of a conserved MAP kinase cascade that includes NRC-1, MEK-2 and MAK-2. During chemotrophic growth in germlings, the MAP kinase cascade members localize to conidial anastomosis tube (CAT) tips every ∼8 minutes, perfectly out of phase with another protein that is recruited to the tip: SOFT, a recently identified scaffold for the MAK-1 MAP kinase pathway in Sordaria macrospora. How the MAK-2 oscillation process is initiated, maintained and what proteins regulate the MAP kinase cascade is currently unclear. A global phosphoproteomics approach using an allele of mak-2 (mak-2Q100G) that can be specifically inhibited by the ATP analog 1NM-PP1 was utilized to identify MAK-2 kinase targets in germlings that were potentially involved in this process. One such putative target was HAM-5, a protein of unknown biochemical function. Previously, Δham-5 mutants were shown to be deficient for hyphal fusion. Here we show that HAM-5-GFP co-localized with NRC-1, MEK-2 and MAK-2 and oscillated with identical dynamics from the cytoplasm to CAT tips during chemotropic interactions. In the Δmak-2 strain, HAM-5-GFP localized to punctate complexes that did not oscillate, but still localized to the germling tip, suggesting that MAK-2 activity influences HAM-5 function/localization. However, MAK-2-GFP showed cytoplasmic and nuclear localization in a Δham-5 strain and did not localize to puncta. Via co-immunoprecipitation experiments, HAM-5 was shown to physically interact with NRC-1, MEK-2 and MAK-2, suggesting that it functions as a scaffold/transport hub for the MAP kinase cascade members for oscillation and chemotropic interactions during germling and hyphal fusion in N. crassa. The identification of HAM-5 as a scaffold-like protein will help to link the activation of MAK-2 cascade to upstream factors and proteins involved in this intriguing process of fungal communication

Изотопный состав подземных вод водосборного бассейна озера Поянху (Китай)

В статье приведены данные об изотопном составе ([delta]D, [delta]18O) подземных вод водосборного бассейна оз. Поянху. По соотношению изотопов [delta]D и [delta]18O определен генезис исследуемых вод, а также проанализировано возможное влияние испарительного концентрирования на формирование их состава. In the article data about isotopic composition ([delta]D, [delta]18O) of the Poyang Lake catchment groundwater is presented. Based on [delta]D and [delta]18O isotopes correlation genesis of the investigated groundwater and influence of evaporation to the groundwater composition are identified

The probability to initiate X chromosome inactivation is determined by the X to autosomal ratio and x chromosome specific allelic properties.

Background: In female mammalian cells, random X chromosome inactivation (XCI) equalizes the dosage of X-encoded gene products to that in male cells. XCI is a stochastic process, in which each X chromosome has a probability to be inactivated. To obtain more insight in the factors setting up this probability, we studied the role of the X to autosome (X:A) ratio in initiation of XCI, and have used the experimental data in a computer simulation model to study the cellular population dynamics of XCI. Methodology/Principal Findings: To obtain more insight in the role of the X:A ratio in initiation of XCI, we generated triploid mouse ES cells by fusion of haploid round spermatids with diploid female and male ES cells. These fusion experiments resulted in only XXY triploid ES cells. XYY and XXX ES lines were absent, suggesting cell death related either to insufficient X-chromosomal gene dosage (XYY) or to inheritance of an epigenetically modified X chromosome (XXX). Analysis of active (Xa) and inactive (Xi) X chromosomes in the obtained triploid XXY lines indicated that the initiation frequency of XCI is low, resulting in a mixed population of XaXiY and XaXaY cells, in which the XaXiY cells have a small proliferative advantage. This result, and findings on XCI in diploid and tetraploid ES cell lines with different X:A ratios, provides evidence that the X:A ratio determines the probability for a given X chromosome to be inactivated. Furthermore, we found that the kinetics of the XCI process can be simulated using a probability for an X chromosome to be inactivated that is proportional to the X:A ratio. These simulation studies re-emphasize our hypothesis that the probability is a function of the concentration of an X-encoded activator of XCI, and of X chromosome specific allelic properties determining the threshold for this activator. Conclusions: The present findings reveal that the probability for an X chromosome to be inactivated is proportional to the X:A ratio. This finding supports the presence of an X-encoded activator of the XCI process. © 2009 Monkhorst et al

Structured specifications for better verification of heap-manipulating programs

Abstract. Conventional specifications typically have a flat structure that is based primarily on the underlying logic. Such specifications lack structures that could have provided better guidance to the verification process. In this work, we propose to add three new structures to a specification framework for separation logic to achieve a more precise and better guided verification for pointer-based programs. The newly introduced structures empower users with more control over the verification process in the following ways: (i) case analysis can be invoked to take advantage of disjointness conditions in the logic. (ii) early, as opposed to late, instantiation can minimise on the use of existential quantification. (iii) formulae that are staged provide better reuse of the verification process. Initial experiments have shown that structured specifications can lead to more precise verification without incurring any performance overhead.