2,363 research outputs found
The Influence of pH Variation on CooA Activity
CooA, a CO-sensing heme protein, acts as a transcriptional activator of CO-metabolizing proteins in bacteria such as Rhodospirillum rubrum and Carboxydothermus hydrogenoformans through sequence-specific DNA binding. Previous research indicated a reduced iron center and CO gas were necessary for CooA to achieve its active conformation and bind DNA. To determine if other reaction conditions facilitate CooA activation, the role of pH on CooA function was tested. Specifically, a fluorescence anisotropy assay was employed to measure possible Fe(III) CooA DNA binding from pH 3 - 12. Interestingly, CooA was observed to bind DNA without CO at acidic conditions, with optimal binding observed at pH ~3. These results are discussed in light of the normal CO-dependent activation mechanism of CooA proteins
Classification of journal surfaces using surface topography parameters and software methods to compensate for stylus geometry
Measurements made with a stylus surface tracer which provides a digitized representation of a surface profile are discussed. Parameters are defined to characterize the height (e.g., RMS roughness, skewness, and kurtosis) and length (e.g., autocorrelation) of the surface topography. These are applied to the characterization of crank shaft journals which were manufactured by different grinding and lopping procedures known to give significant differences in crank shaft bearing life. It was found that three parameters (RMS roughness, skewness, and kurtosis) are necessary to adequately distinguish the character of these surfaces. Every surface specimen has a set of values for these three parameters. They can be regarded as a set coordinate in a space constituted by three characteristics axes. The various journal surfaces can be classified along with the determination of a proper wavelength cutoff (0.25 mm) by using a method of separated subspace. The finite radius of the stylus used for profile tracing gives an inherent measurement error as it passes over the fine structure of the surface. A mathematical model is derived to compensate for this error
The effect of vacuum polarisation on muon-proton scattering at small energies and angles
We give a compact expression for the unpolarised differential cross section
for muon-proton scattering in the one photon exchange approximation. The effect
of adding the vacuum polarisation amplitude to the no-spin-flip amplitude for
one photon exchange is calculated at small energies and scattering angles and
is found to be negligible for present experiments.Comment: 6 pages, one figur
COMPTEL solar flare observations
COMPTEL as part of a solar target of opportunity campaign observed the sun during the period of high solar activity from 7-15 Jun. 1991. Major flares were observed on 9 and 11 Jun. Although both flares were large GOES events (greater than or = X10), they were not extraordinary in terms of gamma-ray emission. Only the decay phase of the 15 Jun. flare was observed by COMPTEL. We report the preliminary analysis of data from these flares, including the first spectroscopic measurement of solar flare neutrons. The deuterium formation line at 2.223 MeV was present in both events and for at least the 9 Jun. event, was comparable to the flux in the nuclear line region of 4-8 MeV, consistent with Solar-Maximum Mission (SSM) Observations. A clear neutron signal was present in the flare of 9 Jun. with the spectrum extending up to 80 MeV and consistent in time with the emission of gamma-rays, confirming the utility of COMPTEL in measuring the solar neutron flux at low energies. The neutron flux below 100 MeV appears to be lower than that of the 3 Jun. 1982 flare by more than an order of magnitude. The neutron signal of the 11 Jun. event is under study. Severe dead time effects resulting from the intense thermal x-rays require significant corrections to the measured flux which increase the magnitude of the associated systematic uncertainties
ProxyNCA++: Revisiting and Revitalizing Proxy Neighborhood Component Analysis
We consider the problem of distance metric learning (DML), where the task is
to learn an effective similarity measure between images. We revisit ProxyNCA
and incorporate several enhancements. We find that low temperature scaling is a
performance-critical component and explain why it works. Besides, we also
discover that Global Max Pooling works better in general when compared to
Global Average Pooling. Additionally, our proposed fast moving proxies also
addresses small gradient issue of proxies, and this component synergizes well
with low temperature scaling and Global Max Pooling. Our enhanced model, called
ProxyNCA++, achieves a 22.9 percentage point average improvement of Recall@1
across four different zero-shot retrieval datasets compared to the original
ProxyNCA algorithm. Furthermore, we achieve state-of-the-art results on the
CUB200, Cars196, Sop, and InShop datasets, achieving Recall@1 scores of 72.2,
90.1, 81.4, and 90.9, respectively.Comment: To appear in the European Conference on Computer Vision (ECCV) 202
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