Design of Electrophoresis Device for Optimation of Dna Visualization and Dna Concentration Using Software

Molecules of deoxyribo nucleic acid (DNA) show a strong polarization allowing for both motions of the dielectrophoresis induced by polarization and electrophoresis based on its negative charge. Considering high subjective and less quantifiable result of the visualization based qualitative test of DNA on gel electrophoresis, designing the tool using a combination of the principles of electrophoresis and dielectrophoresis completed with a software for optimization of DNA visualization and to measure the concentration of small and large–sized DNA fragment is very needed. Accuracy of measurement of DNA concentration using a spectrophotometer UV /VIS is depend on its availability in the laboratory. The aim of this study was to design device for optimization of DNA visualization and measuring the concentration in the gel electrophoresis using MatLab- based software. Experiment using this software measured the concentration of DNA based on its visualization and compared it with calculation obtained from spectrophotometer UV/VIS. The research results showed that the amount of DNA analysed using a spectrophotometer tend to similar with the measurement results using the MatLab-based software although there was differences in quantitative values

National Economic Recovery (PEN) Through Data Strengthening and Digitization of General Trade in Micro, Small, and Medium Enterprises (UMKM) in Indonesia

MSMEs are one of the actors that play a role in the Indonesian economy. The occurrence of Covid-19 greatly impacted the performance of MSMEs. National Economic Recovery (PEN) through strengthening data and digitizing MSMEs is an important thing to do. The aim is to minimize the impact of losses experienced by MSMEs actors if their products are not sold. However, this effort has several obstacles including human resource problems, infrastructure and information systems, network problems and social & behavioral problems

Parameter Optimization of Strategies at CNC end Milling Machine Roland Modela MDX - 40R Cam Against Surface Roughness Made Insoles Shoe Orthotic EVA Rubber Foam

The Quality Optimizing of surface and processing time insole shoe orthotic (iso) that made from EVA rubber foam (erf) is highly dependent on the determination of machining strategies on a CNC milling process. This paper aims to obtain optimal parameters of surface roughness and machining time iso products made erf. Data processing by Taguchi Design of Experiments used to obtain the most significant effect on the Toolpath parameters of machining strategies on software Powermill 2015. The effect of the four parameters (spindle speed, feeding, depth of cut, and types of milling cutter) set out in this experiment would give effect significant surface roughness and machining time iso products made erf. Orthogonal array is L1224, after the output finish analyzed the experimental results

Disparity of Judge's Decision on Children of Narcotics Crime Actors Study on Denpasar State Court Decision No. 3 / Pid.Sus.Anak / 2014 / PN.Dps. by Denpasar State Court Decision No. 14 / Pid.Sus.Anak / 2015 / PN.Dps.

The problem of this research is Why the disparity between the criminal Denpasar State Court Decision No. 3 / Pid.Sus.Anak / 2014 / PN.Dps. by Denpasar State Court Decision No. 14 / Pid.Sus.Anak / 2015 / PN.Dps.? Whether due to the disparity of criminal child Narcotics Crimes between Denpasar State Court Decision No. 3 / Pid.Sus.Anak / 2014 / PN.Dps. by Denpasar State Court Decision No. 14 / Pid.Sus.Anak / 2015 / PN.Dps. ?The method used by researchers is normative juridical law approach and specification in this study were included descriptive analysis. Now sources and types of data in this study are primary data obtained from interviews with field studies Denpasar State Court Judge, and secondary data obtained from the study of literature. Data were analyzed qualitatively. The problems studied by the theory of Pancasila justice, law enforcement and progressive.Based on the results of research that Children judge anyone considering the Research Society (Litmas) Community Advisors (PK) from the Institute of Corrections (Bapas) that, as considered by the Child Justice in case number 14 / Pid.Sus.Anak / 2015 / PN.Dps. Children temporary Judge's decision in case number 3 / Pid.Sus.Anak / 2014 / PN.Dps. not considering it, should judge Children in case number 3 / Pid.Sus.Anak / 2014 / PN.Dps. PK Litmas Bapas expensive, because the perpetrators of the Child is the only user, but instead judge Children in case number 14 / Pid.Sus.Anak / 2015 / PN.Dps. considering Litmas PK Bapas, whereas perpetrators Children in this case proved to be a dealer. Disparities criminal perpetrators of criminal acts against children Narcotics between Denpasar State Court Decision No. 3 / Pid.Sus.Anak / 2014 / PN.Dps. by Denpasar State Court Decision No. 14 / Pid.Sus.Anak / 2015 / PN.Dps. implications for the protection of child neglect

Analysis of Employee Perception About the Whistleblowing System Toward Intention to Conduct Whistleblowing

This research is entitled “Analysis of Employee Perception about the Whistleblowing System toward Intention to Conduct Whistleblowing (The Study on Employees of Purbalingga and Purwokerto Tax Office)”The objective of this research is to examined individual factors that can influence intention to conduct whistleblowing by applying the Theory of Planned Behavior, namely behavioral attitudes, subjective norms and perceived behavioral on the employees of the Purbalingga Tax Office and employees of Purwokerto Tax Office. This research was conducted at the Purbalingga Tax Office and Purwokerto Tax Office. Using variable that explain in Theory of Planned Behavior, the result of this study can be conclude that: (1) there is positive effect of behavioral attitude toward the intention of employee to conduct whistleblowing, (2) there is positive effect of subjective norm toward the intention of employee to conduct whistleblowing information, and (3) there is positive effect of perceived behavioral control toward the intention of employee to conduct whistleblowing

Substitusi Silase Secara Kimiawi Limbah Padat Surimi Ikan Swanggi (Priacanthus Macracanthus) Pada Tepung Ikan Terhadap Retensi Energi Dan Rasio Konversi Pakan Benih Ikan Nila (Oreochromis Niloticus) [Chemical Silage Substitution of Swanggi Surimi Solid Waste (Priacanthus Macracanthus) of Fish Meal Toward Energy Retention and Tiplapia Seeds (Oreochromis Niloticus) Feed Conversion Rate]

Swanggi surimi waste can be processed into economically valuable source of protein through chemical treatment processes produce crude protein content ranging from 51%. Results of high energy retention and reduced feed conversion ratio of solid waste as a substitute swanggi fish surimi fish meal is expected to optimize the growth of tilapia with lower feed prices, thereby reducing the high cost of feed in aquaculture. This study aims to determine the chemical silage substitution of solid waste swanggi fish surimi (P. macracanthus) in fish meal. The experimental design completely randomized design (CRD) with four treatments with five replications. The treatment used is the number of different proteins in each feed rations. The main parameters are observed energy retention (%) and feed conversion ratio. Analysis of data using analysis of variance (ANOVA) and followed Duncan's multiple range test. These results indicate that substitution is chemically silage solid waste swanggi surimi fish in fish meal provides highly significant effect (p <0.05) on the retention of energy and feed conversion ratio tilapia fish. The average retention of the highest energy on a few observations are in treatment P3 (67,90a ± 0.86%) and the lowest at P0 (64,72b ± 1.17%). Average feed conversion ratio was lowest for the treatment P3 (2,41c ± 0.028) and the highest pda P0 (2,50a ± 0.018)

A general secretion signal for the mycobacterial type VII secretion pathway

Mycobacterial pathogens use specialized type VII secretion (T7S) systems to transport crucial virulence factors across their unusual cell envelope into infected host cells. These virulence factors lack classical secretion signals and the mechanism of substrate recognition is not well understood. Here we demonstrate that the model T7S substrates PE25/PPE41, which form a heterodimer, are targeted to the T7S pathway ESX-5 by a signal located in the C terminus of PE25. Site-directed mutagenesis of residues within this C terminus resulted in the identification of a highly conserved motif, i.e., YxxxD/E, which is required for secretion. This motif was also essential for the secretion of LipY, another ESX-5 substrate. Pathogenic mycobacteria have several different T7S systems and we identified a PE protein that is secreted by the ESX-1 system, which allowed us to compare substrate recognition of these two T7S systems. Surprisingly, this ESX-1 substrate contained a C-terminal signal functionally equivalent to that of PE25. Exchange of these C-terminal secretion signals between the PE proteins restored secretion, but each PE protein remained secreted via its own ESX secretion system, indicating that an additional signal(s) provides system specificity. Remarkably, the YxxxD/E motif was also present in and required for efficient secretion of the ESX-1 substrates CFP-10 and EspB. Therefore, our data show that the YxxxD/E motif is a general secretion signal that is present in all known mycobacterial T7S substrates or substrate complexes