X-ray Observations of Neutron Stars and Pulsars: First Results from XMM-Newton

The X-ray Multi-Mirror Mission XMM-Newton is ESA's largest observatory so far; it is dedicated to explore the Universe in the 0.2 - 15 keV X-ray band of the electromagnetic spectrum. Because of its large collecting area very faint sources not accessible before can be observed and it is therefore the long awaited instrument to study young pulsars and neutron stars in supernova remnants, cooling neutron stars and millisecond pulsars at X-ray energies. The high throughput of the instruments, which all are operated simultaneously, provide high resolution spectral, spatial and temporal information from a source during a single observation and make XMM-Newton unique and best suited for pulsar studies. In this article we briefly describe the instrument capabilities useful for pulsar observations and provide information on the timing accuracy on the relative and absolute scale. We further provide scientific results from observations of the Crab-pulsar, PSR J1617-5055 near RCW 103, of young neutron stars in the supernova remnants RX J0852-4622, Puppis-A and RCW 103 including 1E161348-5055.1 which is identified to be the second binary in a supernova remnant. In addition we report on observations of the cooling neutron star PSR B1055-52 and on the millisecond pulsar PSR J0030+0451 which all were observed by XMM-Newton during the first two years of scientific operation.Comment: 23 pages, 22 figures, 4 tables. To appear in the Proceedings of the 270.WE-Heraeus Seminar on Neutron Stars, Pulsars and Supernova Remnants, Jan 21-25, Physikzentrum Bad Honnef, eds W. Becker, H. Lesch & J. Truemper. Proceedings available as MPE-Report 278. The paper with high resolution images can be obtained from ftp://ftp.xray.mpe.mpg.de/people/web/Becker_Aschenbach.pd

Intestinal epithelial responses to Salmonella enterica serovar Enteritidis: Effects on intestinal permeability and ion transport

Salmonella infection of chickens that leads to potential human foodborne salmonellosis continues to be a major concern. Chickens serve as carriers but, in contrast to humans, rarely show any clinical signs including diarrhea. The present investigations aimed to elucidate whether the absence of diarrhea during acute Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) infection may be linked to specific changes in the electrophysiological properties of the chicken gut. Immediately after slaughter, intestinal pieces of the mid-jejunum and cecum of either commercial broiler or specific pathogen-free (SPF) chickens were mounted in Ussing chambers in 2 separate experimental series. Living Salmonella Enteritidis (3 × 109) or Salmonella Enteritidis endotoxin (20 mg/L), or both, were added to the mucosal side for 1 h. In both experimental series, the Salmonella infection decreased the trans-epithelial ion conductance Gt (P < 0.05). In the jejunum of SPF chickens, there was also a marked decrease in net charge transfer across the epithelium, evidenced by decreased short-circuit current (Isc, P < 0.05). Interestingly, the mucosal application of Salmonella endotoxin to the epithelial preparations from jejunum and cecum of SPF chicken had an effect similar to living bacteria. However, the endotoxin had no additional effect on the intestinal function in the presence of bacteria. The decreasing effect of Salmonella and or its endotoxin on Gt could be partly reversed by serosal addition of histamine. To our knowledge, this is the first study to address the functional response of native intestinal epithelium of chicken to an in vitro Salmonella infection. For the first time, it can be reported that intestinal ion permeability of chicken decreases acutely by the presence of Salmonella. This type of response could counteract ion and fluid secretion and may thus, at least in part, explain why chickens do not develop overt diarrhea after Salmonella infection

Campylobacter jejuni colonization promotes the translocation of Escherichia coli to extra-intestinal organs and disturbs the short-chain fatty acids profiles in the chicken gut

For a long time Campylobacter was only considered as a commensal microorganism in avian hosts restricted to the ceca, without any pathogenic features. The precise reasons for the symptomless chicken carriers are still unknown, but investigations of the gastrointestinal ecology of broiler chickens may improve our understanding of the microbial interactions with the host. Therefore, the current studies were conducted to investigate the effects of Campylobacter jejuni colonization on Escherichia coli translocation and on the metabolic end products (short-chain fatty acids, SCFAs). Following oral infection of 14 day old broiler chickens with 1 × 108 CFU of Campylobacter jejuni NCTC 12744 in two independent animal trials, it was found that C. jejuni heavily colonized the intestine and disseminate to extra-intestinal organs. Moreover, in both animal trials, the findings revealed that C. jejuni promoted the translocation of E. coli with a higher number encountered in the spleen and liver at 14 days post infection (dpi). In addition, Campylobacter affected the microbial fermentation in the gastrointestinal tract of broilers by reducing the amount of propionate, isovalerate, and isobutyrate in the cecal digesta of the infected birds at 2 dpi and, at 7 and 14 dpi, butyrate, isobutyrate, and isovalerate were also decreased. However, in the jejunum, the C. jejuni infection lowered only butyrate concentrations at 14 dpi. These data indicated that C. jejuni may utilize SCFAs as carbon sources to promote its colonization in the chicken gut, suggesting that Campylobacter cannot only alter gut colonization dynamics but might also influence physiological processes due to altered microbial metabolite profiles. Finally, the results demonstrated that C. jejuni can cross the intestinal epithelial barrier and facilitates the translocation of Campylobacter itself as well as of other enteric microorganisms such as E. coli to extra-intestinal organs of infected birds. Altogether, our findings suggest that the Campylobacter carrier state in chicken is characterised by multiple changes in the intestinal barrier function, which supports multiplication and survival within the host

On the orientation of the Sagittarius A* system

The near-infrared emission from the black hole at the Galactic center (Sgr A*) has unique properties. The most striking feature is a suggestive periodic sub-structure that has been observed in a couple of flares so far. Using near-infrared polarimetric observations and modelling the quasi-periodicity in terms of an orbiting blob, we try to constrain the three dimensional orientation of the Sgr A* system. We report on so far unpublished polarimetric data from 2003. They support the observations of a roughly constant mean polarization angle of 60 degr \pm 20 degr from 2004-2006. Prior investigations of the 2006 data are deepened. In particular, the blob model fits are evaluated such that constraints on the position angle of Sgr A* can be derived. Confidence contours in the position-inclination angle plane are derived. On a 3sigma level the position angle of the equatorial plane normal is in the range 60 degr - 108 degr (east of north) in combination with a large inclination angle. This agrees well with recent independent work in which radio spectral/morphological properties of Sgr A* and X-ray observations, respectively, have been used. However, the quality of the presently available data and the uncertainties in our model bring some ambiguity to our conclusions.Comment: 10 pages, 4 figures; Research Note accepted by A&A for publicatio

SPEAR Far Ultraviolet Spectral Images of the Cygnus Loop

We present far-ultraviolet (FUV) spectral images, measured at C IV 1550, He II 1640, Si IV+O IV] 1400, and O III] 1664, of the entire Cygnus Loop, observed with the Spectroscopy of Plasma Evolution from Astrophysical Radiation (SPEAR) instrument, also known as FIMS. The spatial distribution of FUV emission generally corresponds with a limb-brightened shell, and is similar to optical, radio and X-ray images. The features found in the present work include a ``carrot'', diffuse interior, and breakout features, which have not been seen in previous FUV studies. Shock velocities of 140-160 km/s is found from a line ratio of O IV] to O III], which is insensitive not only to resonance scattering but also to elemental abundance. The estimated velocity indicates that the fast shocks are widespread across the remnant. By comparing various line ratios with steady-state shock models, it is also shown that the resonance scattering is widespread.Comment: 13 pages, 3 figures, 1 table, accepted for publication in ApJ

ROSAT HRI Observations of the Crab Pulsar: An Improved Temperature upper limit for PSR 0531+21

ROSAT HRI observations have been used to determine an upper limit of the Crab pulsar surface temperature from the off-pulse count rate. For a neutron star mass of 1.4 \Mo and a radius of 10 km as well as the standard distance and interstellar column density, the redshifted temperature upper limit is\/ $T_s^\infty \le 1.55\times 10^6$ K $(3\sigma)$. This is the lowest temperature upper limit obtained for the Crab pulsar so far. Slightly different values for $T_s^\infty$ are computed for the various neutron star models available in the literature, reflecting the difference in the equation of state.Comment: 5 pages, uuencoded postscript, to be published in the Proceedings of the NATO Advanced Study Insitute on "Lives of the Neutron Stars", ed. A. Alpar, U. Kiziloglu and J. van Paradijs ( Kluwer, Dordrecht, 1995 )

Role of physically effective fiber and estimation of dietary fiber adequacy in high-producing dairy cattle

Highly fermentable diets require the inclusion of adequate amounts of fiber to reduce the risk of subacute rumen acidosis (SARA). To assess the adequacy of dietary fiber in dairy cattle, the concept of physically effective neutral detergent fiber (peNDF) has received increasing attention because it amalgamates information on both chemical fiber content and particle size (PS) of the feedstuffs. The nutritional effects of dietary PS and peNDF are complex and involve feed intake behavior (absolute intake and sorting behavior), ruminal mat formation, rumination and salivation, and ruminal motility. Other effects include fermentation characteristics, digesta passage, and nutrient intake and absorption. Moreover, peNDF requirements depend on the fermentability of the starch source (i.e., starch type and endosperm structure). To date, the incomplete understanding of these complex interactions has prevented the establishment of peNDF as a routine method to determine dietary fiber adequacy so far. Therefore, this review is intended to analyze the quantitative effects of and interactions among forage PS, peNDF, and diet fermentability with regard to rumen metabolism and prevention of SARA, and aims to give an overview of the latest achievements in the estimation of dietary fiber adequacy in high-producing dairy cattle. Recently developed models that synthesize the effects of both peNDF and fermentable starch on rumen metabolism appear to provide an appropriate basis for estimation of dietary fiber adequacy in high-producing dairy cows. Data suggest that a period lasting more than 5 to 6h/d during which ruminal pH is <5.8 should be avoided to minimize health disturbances due to SARA. The knowledge generated from these modeling approaches recommends that average amounts of 31.2% peNDF inclusive particles >1.18mm (i.e., peNDF(>1.18)) or 18.5% peNDF inclusive particles >8mm (i.e., peNDF(>8)) in the diet (DM basis) are required. However, inclusion of a concentration of peNDF(>8) in the diet beyond 14.9% of diet DM may lower DM intake level. As such, more research is warranted to develop efficient feeding strategies that encourage inclusion of energy-dense diets without the need to increase their content in peNDF above the threshold that leads to lower DM intake. The latter would require strategies that modulate the fermentability characteristics of the diet and promote absorption and metabolic capacity of ruminal epithelia of dairy cows

Molecular adaptation of ruminal epithelia to highly fermentable diets

Feeding highly fermentable diets to ruminants is one strategy to increase energy intake. The increase in short-chain fatty acid (SCFA) production and reduced ruminal pH associated with highly fermentable diets imposes a challenge to the metabolism and the regulation of intracellular pH homeostasis of ruminal epithelia. The ruminal epithelia respond to these challenges in a coordinated manner. Whereas the enlargement of absorptive surface area is well documented, emerging evidence at the mRNA and transporter and enzyme activity levels indicate that changes in epithelial cell function may be the initial response. It is not surprising that gene expression analysis has identified pathways involved in fatty acid metabolism, ion transport, and intracellular homeostasis to be the pathways dominantly affected during adaptation and after adaptation to a highly fermentable diet. These findings are important because the intraepithelial metabolism of SCFA, particularly butyrate, helps to maintain the concentration gradient between the cytosol and lumen, thereby facilitating absorption. Butyrate metabolism also controls the intracellular availability of butyrate, which is widely regarded as a signaling molecule. Current data indicate that for butyrate metabolism, 3-hydroxy-3 -methylglutaryl-CoA synthase and acetyl-CoA acetyltransferase are potential regulatory points with transient up- and downregulation during diet adaptation. In addition to nutrient transport and utilization, genes involved in the maintenance of cellular tight junction integrity and induction of inflammation have been identified as differentially expressed genes during adaptation to highly fermentable diets. This may have important implications on ruminal epithelial barrier function and the inflammatory response often associated with subacute ruminal acidosis. The objective of this review is to summarize ruminal epithelial adaptation to highly fermentable diets focusing on the changes at the enzyme and transporter activity levels, as well as the underlying molecular changes at the mRNA and protein expression levels

The X-ray Spectrum and Light Curve of Supernova 1995N

We report on multi-epoch X-ray observations of the Type IIn (narrow emission line) supernova SN 1995N with the ROSAT and ASCA satellites. The January 1998 ASCA X-ray spectrum is well fitted by a thermal bremsstrahlung (kT~10 keV, N_H~6e20 cm^-2) or power-law (alpha~1.7, N_H~1e21 cm^-2) model. The X-ray light curve shows evidence for significant flux evolution between August 1996 and January 1998: the count rate from the source decreased by 30% between our August 1996 and August 1997 ROSAT observations, and the X-ray luminosity most likely increased by a factor of ~2 between our August 1997 ROSAT and January 1998 ASCA observations, although evolution of the spectral shape over this interval is not ruled out. The high X-ray luminosity, L_X~1e41 erg/sec, places SN 1995N in a small group of Type IIn supernovae with strong circumstellar interaction, and the evolving X-ray luminosity suggests that the circumstellar medium is distributed inhomogeneously.Comment: MNRAS accepted. 6 pages, 2 figures; uses mn.sty and psfi