Acute retinal necrosis syndrome – a case report

U radu su prikazani dijagnostički postupci i liječenje bolesnice oboljele od akutne retinalne nekroze uzrokovane varicella zoster virusom. Unatoč dugotrajnom liječenju ishod liječenja bio je nepovoljan i bolesnica je ostala trajno slabovidna na bolesno oko. Neke bolesti oka poput akutne retinalne nekroze mogu biti brzog i progresivnog tijeka pa zahtijevaju hitno provođenje dijagnostičkih postupaka koji olakšavaju pravilan i pravovremen izbor načina liječenja. Gubitak vida moguća je posljedica akutne retinalne nekroze, neovisno o etiologiji bolesti. Do gubitka vida češće dolazi u slučaju zakašnjelog liječenja, no gubitak vida moguć je i u slučaju ispravnog i na vrijeme započetog liječenja. Detaljan pregled i obrada ključni su za postavljanje dijagnoze, a problem je što klinička slika može nalikovati neinfekcioznom uveitisu, a također i promjenama u tijeku maligne bolesti.The paper presents diagnostic procedures and treatment of a patient with acute retinal necrosis caused by varicella zoster virus. Despite long-term treatment, the outcome of therapy was poor and the patient remained with permanently impaired vision. Some eye disorders, such as acute retinal necrosis can be rapidly progressive, and therefore require prompt and accurate diagnosis to guide initial therapy. Regardless of the etiology, acute retinal necrosis can lead to vision loss. Vision loss in acute retinal necrosis can occur if proper treatment is delayed or even if it is not delayed. Detailed physical examination and diagnostic procedures are key for reaching the diagnosis, but sometimes it is difficult to distinguish infectious uveitis from non-infectious cause or even manifestations of malignant tumor

Uloga limfadenektomije u bolesnika s karcinomom prostate

Prostate cancer is one of the most important men’s health issues in developed countries. For patients with prostate cancer a preoperative staging of the disease must be made. Involvement of lymph nodes could be assessed using imaging methods (CT or/and MRI), however, newer methods also exist (PET/CT, PSMA PET/CT). For some patients during radical prostatectomy a pelvic lymphadenectomy is recommended. Pelvic lymphadenectomy is indicated in intermediate- and high-risk group patients and with increased probability of lymph node invasion. The most used prediction tools for preoperative assessment of lymph nodes are Briganti and MSKCC nomograms and Partin tables. Pelvic lymphadenectomy can include different lymph nodes group, but extended lymphadenectomy is the recommended procedure. In 1-20% of patients, the lymph node invasion is present. Pelvic lymphadenectomy is primarily a diagnostic and staging method, and in minority of patients with positive lymph nodes it can be a curative method, too. In other patients with positive lymph nodes adjuvant therapy (radiotherapy and androgen deprivation therapy) can be beneficial.Karcinom prostate je jedan od značajnijih zdravstvenih problema muškaraca u razvijenom dijelu svijeta. U bolesnika s dijagnosticiranim karcinomom prostate neophodno je učiniti prijeoperacijsko stupnjevanje bolesti. Zahvaćenost limfnih čvorova se standardno određuje uz pomoć slikovnih metoda (CT i/ili/ MR) iako postoje i novije metode (PET/CT, PSMA PET/CT). U određenog broja bolesnika prilikom radikalne prostatektomije treba učiniti i zdjeličnu limfadenektomiju. Odluka o potrebi za zdjeličnom limfadenektomijom se donosi na osnovu svrstavanja bolesnika u umjerenu odnosno grupu visokoga rizika i ako je vjerojatnost za zahvaćenost limfnih čvorova povećana. Najčešće danas korišteni nomogrami za prijeoperacijsku procjenu zahvaćenosti limfnih čvorova su Briganti i MSKCC nomogram te Partinove tablice. Zdjelična limfadenektomija može obuhvaćati različite skupine limfnih čvorova ali se preporuča učiniti proširenu zdjeličnu limfadenektomiju. U 1-20% bolesnika nalaze se pozitivni limfni čvorovi. Iako zdjelična limfadenektomija ima prvenstveno dijagnostički i prognostički značaj, u manjeg broja bolesnika s pozitivnim limfnim čvorovima može biti i definitivna terapijska metoda. U ostalih bolesnika s pozitivnim limfnim čvorovima adjuvantna terapija (radioterapija i androgen deprivacijska terapija) može biti od terapijskog značaja

Advantages and disadvantages of varicella and herpes zoster vaccination

Vodene kozice (VK) su česta akutna zarazna bolest s načelno dobrom prognozom. Bolest može biti teška i životno ugrožavajuća. Može se spriječiti cijepljenjem koje se u nekim zemljama (SAD, Australija) u okviru univerzalnog imunizacijskog programa provodi desetljećima. Obavezno cijepljenje djece protiv VK u Europi nije uobičajeno. Herpes-zoster (HZ) nastaje nakon reaktivacije varicella-zoster virusa (VZV) koji nakon primarne infekcije trajno ostaje u organizmu. I HZ je bolest uglavnom dobre prognoze, ali i potencijalno ozbiljnih komplikacija među kojima se ističe postherpetička neuralgija. I ova bolest se može spriječiti cijepljenjem. U radu su opisane mogućnosti te prednosti i nedostaci cijepljenja protiv VK i HZ. Uvođenje cijepljenja treba poticati posebno za rizične skupine u kojih se može očekivati razvoj teških oblika bolesti. Daljnja istraživanja učinkovitosti trenutnih atenuiranih, ali i novih umrtvljenih cjepiva te alternativnih putova primjene i potrebe docjepljivanja proširit će spoznaje o utjecaju na pojavnost teških kliničkih slika i komplikacija VK i i HZ.Varicella is a common acute infectious disease, generally with a good prognosis. However, varicella may present as severe and life threatening disease. Varicella is a vaccine-preventable disease and varicella vaccination has been carried out in some countries (USA, Australia) for decades. Mandatory childhood vaccination against varicella is not common in Europe. After primary infection varicella zoster virus (VZV) stays within the host permanently and VZV reactivation causes herpes zoster (HZ). HZ is also a disease with generally good prognosis, but also with potentialy severe complications, and the most distinguished one is postherpetic neuralgia. HZ is also a vaccine-preventable disease. This paper describes prophylactic possibilities against varicella and HZ and their advantages and disadvantages. Vaccine implementation should be encouraged, especially vaccination of individuals who are at high risk of developing severe disease. Further studies on attenuated vaccines and also on new recombinant vaccines, new route of vaccine administration and booster vaccination will bring new knowledge on vaccine impact on severe disease incidence and VZV and HZ complications

Nurse attitudes in Croatia towards primary resuscitation of prematurely born infants

CILJ: Cilj istraživanja bilo je ispitati stavove medicinskih sestara u Hrvatskoj o primarnom oživljavanju prijevremeno rođene djece. METODE: Pisanom anketom ispitivana je dob, roditeljstvo, radno iskustvo i mjesto rada te stavovi medicinskih sestara iz Hrvatske o primarnom oživljavanju prijevremeno rođene djece. Anketirano je 174 medicinskih sestara; 49 zaposlenih u rađaonici ili na mjestima rada sa trudnicama (Rađ/Tr), 79 u pedijatrijskim/ neonatološkim jedinicama intenzivnog liječenja (JIL/NICU), 46 na radnom mjestu sa općom populacijom (OP). REZULTATI: Nije bilo statistički značajne razlike među skupinama u odnosu na dob, materinstvo i duljinu radnog staža. Postoji statistički značajna razlika u stavovima sestara zaposlenih u Rađ/Tr te u JIL/NICU u odnosu na sestre zaposlene na mjestima rada sa OP u 22. i 23. tjednu gestacije. Postoji statistički značajna razlika u stavovima sestara zaposlenih u JIL/NICU u odnosu na sestre zaposlene na mjestima rada sa OP u 24., 25. i 26. tjednu gestacije. Nema razlike u stavovima sestara JIL/NICU-a u odnosu na sestre zaposlene u Rađ/Tr. RASPRAVA: Medicinske sestre na mjestu rada sa OP sklonije su pristupanju primarnog oživljavanja prijevremeno rođene novorođenčadi u odnosu na njihove kolegice u komparativnim skupinama. Ovakav je rezultat posljedica činjenice da sestre zaposlene u JIL/NICU i Rađ/Tr imaju drugačije iskustvo u svezi primijen terapijskih i dijagnostičkih postupaka s ciljem održavanja života novorođenčadi rođene na granici preživljenja. ZAKLJUČAK: Radno mjesto, tj. iskustvo stečno na specifičnom radnom mjestu, a ne suvremeni stavovi i preporuke kliničke bioetike, osnova su u stvaranju stavova o primarnom oživljavanju prijevremeno rođene novorođenčadi. Edukacije iz područja bioetičkih stavova mora bit trajna i u skladu sa suvremenim mjerama intezivnog liječenja i dugoročnog ishoda prijevremeno rođene novorođenčadi.AIM: The aim was to determine the attitudes of nurses in Croatia toward primary resuscitation of prematurely born infants. METHODS: A written questionnaire was distributed to the nursing staff wiith questions regarding their age, parity, work experience, work place and attitudes toward primary resuscitation of prematurely born infants. A total of 174 nurses completed the questionnaire; 49 of them working at the delivery room or in contact with pregnant women (DR/PW), 79 working at the pediatric/neonatal intensive care units (PICU/NICU), 46 working with the general population (GP). RESULTS: There were no statistically significant differences regarding age, parity and work experience among groups, nor in attitudes among DR/PW and PICU/NICU nurses There was a statistically significant difference in attitudes toward primary resuscitation of infants born at 22nd and 23rd gestational week among DR/PW – PICU /NICU and GP nurses, as well as in attitudes toward primary resuscitation of infants born at 24th to 26th gestational week among PICU /NICU and GP nurses. DISCUSSION: GP nursing staff has attitude to initiate more likely primary resuscitation of premature born infants than their colleagues in the comparative groups. Such results are consequences of the fact that PICU/NICU-DR/PW nurses have different experience towards the complexity of procedures applied in primary resuscitation of infants who are born at the margins of viability. CONCLUSIONS: Working place i.e. experience at the particular working place, but not modern guidance in clinical bioethics, play major role in creation of attitude towards the primary resuscitation of prematurely born infants among nurses. Education in clinical bioethics should be continuous, in accordance with medical advances in neonatal intensive care and long term outcome of prematurely born infants

Presječna studija dinamike temperature izdaha nakon popušene cigarete i njezina povezanost s promjenama plućne funkcije u pušača prediktivnima za rizik od KOPB-a

Exhaled breath temperature (EBT) is a biomarker of inflammation and vascularity of the airways already shown to predict incident COPD. This cross-sectional study was aimed to assess the potential of EBT in identifying “healthy” smokers susceptible to cigarette smoke toxicity of the airways and to the risk of developing COPD by analysing the dynamics of EBT after smoking a cigarette and its associations with their demographics (age, smoking burden) and lung function. The study included 55 current smokers of both sexes, 29–62 years of age, with median smoking exposure of 15 (10–71.8) pack-years. EBT was measured at baseline and 5, 15, 30, 45, and 60 min after smoking a single cigarette. Lung function was measured with spirometry followed by a bronchodilator test. To compare changes in EBT between repeated measurements we used the analysis of variance and the area under the curve (EBTAUC) as a dependent variable. Multivariate regression analysis was used to look for associations with patient characteristics and lung function in particular. The average (±SD) baseline EBT was 33.42±1.50 °C. The highest significant increase to 33.84 (1.25) °C was recorded 5 min after the cigarette was smoked (p=0.003), and it took one hour for it to return to the baseline. EBTAUC showed significant repeatability (ICC=0.85, p<0.001) and was significantly associated with age, body mass index, number of cigarettes smoked a day, baseline EBT, and baseline FEF75 (R2=0.39, p<0.001 for the model). Our results suggest that EBT after smoking a single cigarette could be used as early risk predictor of changes associated with chronic cigarette smoke exposure.Temperatura izdaha (EBT) biomarker je upale i vaskularizacije dišnih putova za koji je utvrđeno da može predvidjeti nastanak KOPB-a. Cilj ove presječne studije bio je procijeniti potencijal EBT-a u identifikaciji “zdravih” pušača čiji su dišni putovi osjetljivi na toksični učinak duhanskoga dima uz rizik od nastanka KOPB-a. Taj se cilj planirao postići utvrđivanjem povezanosti dinamike EBT-a nakon popušene cigarete s demografskim karakteristikama (dob, spol, pušačka navika) i plućnom funkcijom ispitanika. U ispitivanje je bilo uključeno 55 aktivnih pušača obaju spolova u dobi od 29 do 62 godine, čiji je medijan izloženosti duhanskom dimu bio 15 (10 – 71,8) pušačkih godina (pack-years). EBT je mjeren na početku te 5, 15, 30, 45 i 60 minuta nakon popušene cigarete. Plućnu funkciju mjerili smo spirometrijski, uz susljedni bronhodilatacijski test. Za analizu dinamike EBT-a koristili smo se analizom varijance te smo izračunali površinu ispod krivulje u odnosu na početno mjerenje (EBTAUC) te EBTAUC upotrijebili kao zavisnu varijablu. Multivarijatna regresijska analiza korištena je za utvrđivanje povezanosti EBTAUC s karakteristikama ispitanika, posebice s plućnom funkcijom. Prosječni (±SD) izmjereni početni EBT bio je 33,42±1,50 °C, a najveće značajno izmjereno povećanje zabilježeno je 5 minuta nakon popušene cigarete (33,84 (1,25) °C, p=0,003). Bilo je potrebno 60 minuta kako bi se EBT vratio na početnu vrijednost. Utvrđena je značajna ponovljivost EBTAUC (ICC=0,85, p<0,001). Ispitivanjem je utvrđeno je da je EBTAUC značajno nezavisno povezan s dobi, indeksom tjelesne mase, brojem popušenih cigareta dnevno, početnim EBT-om te s početnim FEF75 (R2=0,39, p<0,001 za model). Rezultati ove studije sugeriraju da EBT nakon popušene cigarete može biti koristan kao rani prediktor rizika od promjena koje su povezane s kroničnom izloženosti duhanskom dimu

Mutual protection of ribosomal proteins L5 and L11 from degradation is essential for p53 activation upon ribosomal biogenesis stress

Impairment of ribosomal biogenesis can activate the p53 protein independently of DNA damage. The ability of ribosomal proteins L5, L11, L23, L26, or S7 to bind Mdm2 and inhibit its ubiquitin ligase activity has been suggested as a critical step in p53 activation under these conditions. Here, we report that L5 and L11 are particularly important for this response. Whereas several other newly synthesized ribosomal proteins are degraded by proteasomes upon inhibition of Pol I activity by actinomycin D, L5 and L11 accumulate in the ribosome-free fraction where they bind to Mdm2. This selective accumulation of free L5 and L11 is due to their mutual protection from proteasomal degradation. Furthermore, the endogenous, newly synthesized L5 and L11 continue to be imported into nucleoli even after nucleolar disruption and colocalize with Mdm2, p53, and promyelocytic leukemia protein. This suggests that the disrupted nucleoli may provide a platform for L5- and L11-dependent p53 activation, implying a role for the nucleolus in p53 activation by ribosomal biogenesis stress. These findings may have important implications with respect to understanding the pathogenesis of diseases caused by impaired ribosome biogenesis

BIOACTIVE PHENOLIC COMPOUNDS IN THE TREATMENT OF EXPERIMENTAL ULCERATIVE COLITIS AND COLON CANCER

Uvod: Ulcerozni kolitis (UK) je kronična bolest koja nastaje kao posljedica neodgovarajućeg imunološkog odgovora. Rak debelog crijeva treći je najčešće dijagnosticirani zloćudni tumor i drugi uzrok smrtnosti. Dosadašnje terapije ovih bolesti nisu se pokazale odgovarajućim. Klorogenična kiselina i luteolin su polifenolni spojevi s pokazanim značajnim farmakološkim učincima u tretmanu različitih bolesti. Cilj istraživanja: Cilj rada je ispitati i predložiti mehanizam djelovanja klorogenične kiseline i luteolina u terapiji UK i raka debelog crijeva. Materijal i metode: Istraživanje je provedeno na in vivo modelu kolitisa i na in vitro modelu raka debelog crijeva. Tijekom razvoja kolitisa praćene su kliničke promjene, a stupanj oštećenja tkiva debeloga crijeva određen je histopatološkom analizom. Izražaj istraživanih proteina određen je Western blot metodom i imunohistokemijski. Uloga signalnog puta izvanstaničnim signalima reguliranih kinaza (ERK) istražena je primjenom inhibitora. Citotoksičnost ispitivanih spojeva određena je testom stanične vijabilnosti i sposobnosti stvaranja kolonija. Autofagija i apoptoza analizirane su fluorescencijski. Uloga autofagije ispitana je primjenom inhibitora autofagije. Rezultati: Klorogenična kiselina i luteolin ublažavaju kliničke simptome i smanjuju intenzitet upale. Klorogenična kiselina smanjuje aktivaciju proteina signalnih puteva proteinskih kinaza aktiviranih mitogenom (MAPK), proteinske kinaze B (PKB (Akt)) i pretvarača signala i aktivatora transkripcije 3 (STAT3) te djeluje antioksidacijski. Luteolin aktivira ERK protein. Klorogenična kiselina ne utječe na vijabilnost stanica, dok luteolin smanjuje njihovu vijabilnost i proliferaciju te djeluje proapoptotski, proautofagično i antimetastatski. Zaključak: Istraživanjem je pokazan protuupalni i antiapoptotski učinak klorogenične kiseline u UK te protuupalni, antiapoptotski i antiautofagični učinak luteolina koji je posredovan ERK signalnim putem. Antikarcinogena aktivnost luteolina se temelji na ERK/FOXO3a ovisnom mehanizmu i antimetastatskom potencijaluIntroduction: Ulcerative colitis (UC) is a chronic disease that results from an inadequate immune response. Colon cancer is the third most commonly diagnosed malignancy and the second leading cause of death. Previous therapies for these diseases have not been shown to be adequate. Chlorogenic acid and luteolin are polyphenolic compounds with a shown significant pharmacological effects in the treatment of various diseases. Aim of the study: The aim of this study was to investigate and propose the mechanism of action of chlorogenic acid and luteolin in the treatment of UC and colon cancer. Matherial and methods: The study was performed in an in vivo model of colitis and in an in vitro model of colon cancer. During the development of colitis, clinical changes were monitored and the degree colon tissue damage was determined by histopathological analysis. The expression of the investigated proteins was determined by the Western blot method and immunohistochemically. The role of the extracellular regulated kinases (ERK) signaling pathway was investigated using inhibitor. The cytotoxicity of the test compounds was determined by a cell viability and colony formation assay. Autophagy and apoptosis were analyzed by fluorescence. The role of autophagy was investigated using autophagy inhibitor. Results: Chlorogenic acid and luteolin alleviate clinical symptoms and reduce the intensity of inflammation. Chlorogenic acid reduces the activation of mitogen-activated protein kinases (MAPK), protein kinase B (PKB (Akt)) and signal transducer and transcription activator 3 (STAT3) signaling pathway proteins and has an antioxidant effect. Luteolin activates ERK protein. Chlorogenic acid does not affect cell viability, while luteolin reduces their viability and proliferation and has proaptotic, proautophagic and antimetastatic effect. Conclusion: The study demonstrated the antiinflammatory and antiapoptotic effect of chlorogenic acid in the UC and the antiinflammatory, antiapoptotic and antiautophagic effect of luteolin mediated by the ERK signaling pathway. The anticarcinogenic activity of luteolin is based on the ERK/FOXO3a dependent mechanism and antimetastatic potential

BIOACTIVE PHENOLIC COMPOUNDS IN THE TREATMENT OF EXPERIMENTAL ULCERATIVE COLITIS AND COLON CANCER

Uvod: Ulcerozni kolitis (UK) je kronična bolest koja nastaje kao posljedica neodgovarajućeg imunološkog odgovora. Rak debelog crijeva treći je najčešće dijagnosticirani zloćudni tumor i drugi uzrok smrtnosti. Dosadašnje terapije ovih bolesti nisu se pokazale odgovarajućim. Klorogenična kiselina i luteolin su polifenolni spojevi s pokazanim značajnim farmakološkim učincima u tretmanu različitih bolesti. Cilj istraživanja: Cilj rada je ispitati i predložiti mehanizam djelovanja klorogenične kiseline i luteolina u terapiji UK i raka debelog crijeva. Materijal i metode: Istraživanje je provedeno na in vivo modelu kolitisa i na in vitro modelu raka debelog crijeva. Tijekom razvoja kolitisa praćene su kliničke promjene, a stupanj oštećenja tkiva debeloga crijeva određen je histopatološkom analizom. Izražaj istraživanih proteina određen je Western blot metodom i imunohistokemijski. Uloga signalnog puta izvanstaničnim signalima reguliranih kinaza (ERK) istražena je primjenom inhibitora. Citotoksičnost ispitivanih spojeva određena je testom stanične vijabilnosti i sposobnosti stvaranja kolonija. Autofagija i apoptoza analizirane su fluorescencijski. Uloga autofagije ispitana je primjenom inhibitora autofagije. Rezultati: Klorogenična kiselina i luteolin ublažavaju kliničke simptome i smanjuju intenzitet upale. Klorogenična kiselina smanjuje aktivaciju proteina signalnih puteva proteinskih kinaza aktiviranih mitogenom (MAPK), proteinske kinaze B (PKB (Akt)) i pretvarača signala i aktivatora transkripcije 3 (STAT3) te djeluje antioksidacijski. Luteolin aktivira ERK protein. Klorogenična kiselina ne utječe na vijabilnost stanica, dok luteolin smanjuje njihovu vijabilnost i proliferaciju te djeluje proapoptotski, proautofagično i antimetastatski. Zaključak: Istraživanjem je pokazan protuupalni i antiapoptotski učinak klorogenične kiseline u UK te protuupalni, antiapoptotski i antiautofagični učinak luteolina koji je posredovan ERK signalnim putem. Antikarcinogena aktivnost luteolina se temelji na ERK/FOXO3a ovisnom mehanizmu i antimetastatskom potencijaluIntroduction: Ulcerative colitis (UC) is a chronic disease that results from an inadequate immune response. Colon cancer is the third most commonly diagnosed malignancy and the second leading cause of death. Previous therapies for these diseases have not been shown to be adequate. Chlorogenic acid and luteolin are polyphenolic compounds with a shown significant pharmacological effects in the treatment of various diseases. Aim of the study: The aim of this study was to investigate and propose the mechanism of action of chlorogenic acid and luteolin in the treatment of UC and colon cancer. Matherial and methods: The study was performed in an in vivo model of colitis and in an in vitro model of colon cancer. During the development of colitis, clinical changes were monitored and the degree colon tissue damage was determined by histopathological analysis. The expression of the investigated proteins was determined by the Western blot method and immunohistochemically. The role of the extracellular regulated kinases (ERK) signaling pathway was investigated using inhibitor. The cytotoxicity of the test compounds was determined by a cell viability and colony formation assay. Autophagy and apoptosis were analyzed by fluorescence. The role of autophagy was investigated using autophagy inhibitor. Results: Chlorogenic acid and luteolin alleviate clinical symptoms and reduce the intensity of inflammation. Chlorogenic acid reduces the activation of mitogen-activated protein kinases (MAPK), protein kinase B (PKB (Akt)) and signal transducer and transcription activator 3 (STAT3) signaling pathway proteins and has an antioxidant effect. Luteolin activates ERK protein. Chlorogenic acid does not affect cell viability, while luteolin reduces their viability and proliferation and has proaptotic, proautophagic and antimetastatic effect. Conclusion: The study demonstrated the antiinflammatory and antiapoptotic effect of chlorogenic acid in the UC and the antiinflammatory, antiapoptotic and antiautophagic effect of luteolin mediated by the ERK signaling pathway. The anticarcinogenic activity of luteolin is based on the ERK/FOXO3a dependent mechanism and antimetastatic potential

JAVA WEB ALAT ZA IZRADU STATIČKE HTML STRANICE

Cilj ovog završnog rada je stvaranje alternative za platforme za izradu web stranica kao što su Wix i Squarespace gdje će korisnik biti u mogućnosti da izveze konačni rezultat u obliku HTML i CSS datoteka što nije bilo moguće s već postojećim platformama za stvaranje web stranica. Također će biti moguće da korisnik određuje raspored elemenata web stranice kroz crtež koji može biti nacrtan ručno ili kroz neki program sličan MS Paint. Prvi korak u stvaranju web stranice je stvaranje slike i njeno učitavanje. Sliku se zatim procesira kroz backend pisan u programskom jeziku Java uz korištenje biblioteke OpenCV. Drugi korak će biti prikaz generiranih HTML i CSS datoteka uz mogućnost da korisnik dodatno podesi raspored i veličinu elemenata.Main goal of this final thesis is to create an alternative solution for building web sites similar to Wix and Squarespace which would allow user to export the final result as HTML and CSS files which wasn't possible in already existing website bulder platforms. It's also going to be possible for user to arrange the layout through drawing which can be drawn manually pr by using software like MS Paint. First step in creating a web page is creating a picture and uploading it. Afterwards, the picture is being processed through backend wiritten in Java combined with OpenCV library. Second step is going to be preview of generated HTML and CSS file with option that allows user to additionally rearrange the layout