85 research outputs found
Refugiados en Serbia: de camino hacia una vida mejor
MĆ”s de 450 000 personas pasaron a travĆ©s de Serbia desde que comenzĆ³ 2015 hasta mediados de noviembre. Sin embargo, en 2014 las cifras fueron ya elevadas e iban en aumento
Effects of subacute dibutyl phthalate exposure on UDP-glucuronosyltransferase 1A9 expression in rat liver : [abstract]
Subacute exposure to dibutyl phthalate results in an increased number of ovarian corpus luteum in female wistar rats : [abstract]
Effect of subacute dibutyl phthalate treatment on the activity and expression of antioxidant enzymes in rat liver : [abstract]
Antigenotoxic effect of plant extracts
This report reviews our study of antigenotoxic compounds from medicinal and aromatic plants performed over several years. The studies of this type are aimed at understanding the protective mechanisms which may be relevant for the primary prevention of cancer and other mutation-related diseases. Antigenotoxic potential in this study is estimated with prokaryotic and eukaryotic tests measuring spontaneous and induced mutations, recombination, mutagenic repair, chromosomal aberrations and micronuclei. Our results indicate that monoterpenoids from sage act as modulators of DNA repair pathways, whereas sage antioxidants interfere with metabolic activation enzymes. The potential use of sage extracts in cancer prevention is discussed
The anthropogenic impact on water quality of the river Danube in Serbia: Microbiological analysis and genotoxicity monitoring
The aim of this work was to examine the impact of urban wastewaters on the water quality of the Danube River in Serbia. Samples of water and sediments for microbiological analysis and genotoxicity monitoring were collected from 6 sites during spring and/or autumn 2010. Sanitary analysis, i.e. enumeration of total and fecal coliforms and intestinal enterococci, indicated moderate to critical fecal contamination, while organic load assessment (oligotroph to heterotroph ratio, index of phosphatase activity) revealed the category of moderately polluted water. Mercury-resistant bacteria were detected in all water samples, with high numbers at locations positioned downstream of Belgrade. There was no correlation of the microbiological parameters of the sediment and water samples. Genotoxicity monitoring, performed by the comet assay on hemocytes of mussels Sinanodonta woodiana, indicated a significant increase of DNA damage in mussels collected from the studied sites compared with the control group.Projekat ministarstva br. 173025 i br. 4300
Molecular Mechanisms of Action of Antimutagens from Sage (Salvia officinalis) and Basil (Ocimum basilicum)
Geographical and biological analysis of the water quality of Bovan Lake, Serbia
In this work we performed a complex geographical analysis of the Bovan Lake basin, Serbia, as well as an analysis of the physical, chemical and biological parameters of water reservoir during a one-year period. The basic sanitary characteristics and physical, chemical and biological parameters, necessary for understanding locality conditions, were studied, and the saprobity index, class of quality, index O/H, degree of saprobity, degree of trophicity and category based on the phosphatase activity index (PAI), were determined. Our results classify the ecosystem as polytrophic. The degree of saprobity is oligosaprobic - beta mesosaprobic, with a catarobic degree in winter, classifying the water as III - IV class of quality. Due to the presence of organic residues, a domination of heterotrophic bacteria in comparison to oligotrophic bacteria is evident. The ability of autopurification is weak. The results indicate the need for continual monitoring of the water quality and environmental protection of Bovan Lake
Ispitivanje antivirusne aktivnosti ekstrakata žalfije Salvia officinalis L. (Lamiaceae)
In the present study, we examined cytotoxicity and extracellular and intracellular antiviral activity of fracĀtionated extracts of wild and cultivated sage Salvia officinalis L. (Lamiaceae) in vitro using the WISH-VSV model system. Extracts were obtained by fractionating depigmented ethanol extracts of sage plants with supercritical CO2 at different pressures. Cytotoxicity was determined by examining cellular morphology in situ with the aid of a colorimetric micromethod and by cell staining with trypan blue. The fraction of distilled cultivated sage obtained at CO2 pressure of 300 bars and temperature of 60Ā°C (149/3) was the most cytotoxic, with CTD10 44 Ī¼g/ml. That of non-distilled cultivated sage obtained at CO2 pressure of 500 bars and temperature of 100Ā°C (144/5) was the least toxic (CTD10 199 Ī¼g/ml). Moreover, 144/5 had an antiviral effect at the intracellular level: when added 5 hours before VSV infection, it caused 100% reduction of CPE at concentrations of 99.5 and 199.0 Ī¼g/ml; when added after virus penetration had occurred, the same concentrations caused 35 and 60% reduction, respectively. The obtained results indicate that antiviral activity of 144/5 involves inhibition of the early steps of the virus infective cycle without a direct virucidal effect. Abbreviations: WISH - human amnion epithelial cells, VSV - vesicular stomatitis virus, HSV - herpes simplex virus, CPE - cytopathic effect, IS - selectivity index, TCID50 - tissue culture infective dose, CTD10 - 10% cytotoxic concentrations.U radu je ispitivana antiviralna aktivnost razliÄito frakcionisanih ekstrakata divlje i gajene žalfije Salvia officinalis L. (Lamiaceae) u in vitro uslovima koristeÄi WISH-VSV model sistem. Ekstrakti su dobijeni frakcionisanjem depigmentisanog etanolnog biljnog ekstrakta pod razliÄitim pritiskom CO2. CitotoksiÄnost je odreÄivana praÄenjem Äelijske morfologije in situ, i bojenjem Äelija sa tripan plavim. Frakcija gajene žalfije dobijena na CO2 pritisku od 300 bara i temperaturi od 60Ā°C (149/3) je pokazala najveÄu citotoksiÄnost (CTD10 44 Ī¼g/ml). Frakcija nedestilisane gajene žalfije dobijena na CO2 pritisku od 500 bara i temperaturi od 100Ā°C (144/5) je pokazala najmanju toksiÄnost (CTD10 199 Ī¼g/ml). TakoÄe, frakcija 144/5 je pokazala i antiviralni efekat na intracelularnom nivou: kada se Äelije tretiraju 5 sati pre VSV infekcije, redukcija CPE je bila 100% na koncentraciji od 99.5 i 199.0 Ī¼g/ml; kada se Äelije tretiraju posle ulaska virusa u Äeliju na istim koncentarcijama redukcija CPE izĀnosi 35% odnosno 60%. Dobijeni rezultati ukazuju da frakcija 144/5 ima antiviralnu aktivnost koja se ostvaruje krozinhibiciju ranih stupnjeva viralne infekcije bezdirektnog virucidalnog efekta
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