Andrić Nebojša

Ivelja Ivana

Kojić Danijela

Marković Filipović Jelena

Vukčević Jelena

English

University of Szeged

29th International Symposium on Analytical and Environmental Problems  159  EFFECT OF SUBACUTE DIBUTYL PHTHALATE TREATMENT ON THE ACTIVITY AND EXPRESSION OF ANTIOXIDANT ENZYMES IN RAT LIVER   Ivana Ivelja, Jelena Vukčević, Danijela Kojić, Nebojša Andrić, Jelena Marković Filipović  Department of biology and ecology, Faculty of Science, University of Novi Sad, 21000 Novi Sad, Trg Dositeja Obradovića 3, Serbia e-mail: ivana.ivelja@dbe.uns.ac.rs  Abstract Di-n-butyl phthalate (DBP), a common plastic additive, is prone to leaching and evaporating from plastic products due to its weak chemical bonds, resulting in its widespread presence in the environment. The aim of our study was to investigate whether DBP applied orally in various doses disturbs the activity and expression of antioxidant enzymes in rat liver . A total of 30 female Wistar rats were divided into 4 groups and exposed to DBP in food in doses of 0, 100, 500, and 5000 mg/kg diet, for 28 days. After the study period, tissue samples were collected for evaluating the antioxidant capacity and for RNA extraction. Total superoxide dismutase (SOD) and catalase (CAT) activities were measured spectrophotometrically by monitoring changes in absorbance over the 3min interval, while the expression of the Sod1, Sod2 and Cat were obtained by real-time RT-PCR. Our findings revealed that total SOD activity was notably lower in groups treated with 100 and 500 mg DBP/kg diet. Conversely, CAT activity significantly increased in groups exposed to 500 and 5000 mg DBP/kg compared to the control group. However, our RT-qPCR analysis showed no statistically significant changes in the expression of corresponding genes. Our results indicate that subacute dibytil phthalate treatment causes adverse effects on the activity of antioxidant enzymes, potentially leading to oxidative damage.   Acknowledgements This work was supported by the Provincial Secretariat for Higher Education and Scientific Research of the Autonomous Province of Vojvodina [Grant No. 142-451-3164/2023-01).    

Effect of subacute dibutyl phthalate treatment on the activity and expression of antioxidant enzymes in rat liver : [abstract]

http://acta.bibl.u-szeged.hu/82406/1/proceedings_of_isaep_2023_159.pdf

Effect of subacute dibutyl phthalate treatment on the activity and expression of antioxidant enzymes in rat liver : [abstract]

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