Sastav i struktura zajednica makrozoobentosa mediteranske krške rijeke Cetine i njene pritoke Rude

Benthic invertebrate communities of the Cetina River and the upper reach of the Ruda River were sampled monthly at 11 sites from August 2004 to August 2005. The sampling sites were located along the longitudinal gradient of the Cetina and in the upper reach of the Cetina\u27s largest tributary, the Ruda River. Throughout the study, a total of 310225 animals were collected in 366 samples. In the upper and middle reaches the most numerous taxa were Amphipoda and Gastropoda, while the most numerous taxon in the lower reaches was Gastropoda. The highest and the lowest population density of benthic invertebrates were recorded at the sites Preočki Most in June 2005 (22728 individuals/m2) and Radmanove Mlinice in November 2004 (90 individuals/m2), respectively. At sites Trilj I and Trilj II, the most numerous taxa were Oligochaeta and Chironomidae, indicators of watercourses with higher organic loads. The composition of benthic invertebrate communities at sites along the entire course of Cetina does not reflect the situation expected under natural conditions due to flow regulation and construction of hydroelectric power plants and reservoirs.Istraživanje bentičkih beskralježnjaka rijeke Cetine i izvorišnog dijela toka rijeke Rude provedeno je u razdoblju od kolovoza 2004. do kolovoza 2005. godine. Cilj istraživanja bio je utvrditi sastav i strukturu bentičkih beskralježnjaka duž toka rijeke Cetine i izvorišnog dijela rijeke Rude. U razdoblju od 13 mjeseci u 366 uzoraka ukupno je prikupljeno 310225 jedinki bentičkih beskralježnjaka. Najbrojnije su bile skupine Amphipoda i Gastropoda, i to u gornjem i srednjem dijelu toka, dok je u donjem dijelu toka najbrojnija skupina bila Gastropoda. Udio predstavnika čistih voda iz skupina Plecoptera, Ephemeroptera i Trichoptera smanjivao se od izvora prema ušću. Najveća gustoća bentičkih beskralježnjaka bila je na gornjem dijelu toka, na postaji Preočki most u lipnju 2005. godine (22728 jedinki/m2), a najmanja na postaji na donjem dijelu toka, Radmanovim mlinicama, u studenom 2004. godine (90 jedinki/m2). Na postajama Trilj I i Trilj II najzastupljenije su bile skupine Oligochaeta i Chironomidae koje su karakteristične za organski opterećenije vodotoke. Uzrok tome je vjerojatno neriješena odvodnja otpadnih i komunalnih voda grada Trilja i okolnih područja. Sastav zajednica makrozoobentosa na postajama duž cijelog toka rijeke Cetine ne odražava situaciju koju bismo očekivali u prirodnim uvjetima, zbog regulacije samog toka i izgradnje hidroelektrana i akumulacija

Insight into the mechanism of ferroptosis inhibition by ferrostatin-1

Ferroptosis is a form of cell death primed by iron and lipid hydroperoxides and prevented by GPx4. Ferrostatin-1 (fer-1) inhibits ferroptosis much more efficiently than phenolic antioxidants. Previous studies on the antioxidant efficiency of fer-1 adopted kinetic tests where a diazo compound generates the hydroperoxyl radical scavenged by the antioxidant. However, this reaction, accounting for a chain breaking effect, is only minimally useful for the description of the inhibition of ferrous iron and lipid hydroperoxide dependent peroxidation. Scavenging lipid hydroperoxyl radicals, indeed, generates lipid hydroperoxides from which ferrous iron initiates a new peroxidative chain reaction. We show that when fer-1 inhibits peroxidation, initiated by iron and traces of lipid hydroperoxides in liposomes, the pattern of oxidized species produced from traces of pre-existing hydroperoxides is practically identical to that observed following exhaustive peroxidation in the absence of the antioxidant. This supported the notion that the anti-ferroptotic activity of fer-1 is actually due to the scavenging of initiating alkoxyl radicals produced, together with other rearrangement products, by ferrous iron from lipid hydroperoxides. Notably, fer-1 is not consumed while inhibiting iron dependent lipid peroxidation. The emerging concept is that it is ferrous iron itself that reduces fer-1 radical. This was supported by electroanalytical evidence that fer-1 forms a complex with iron and further confirmed in cells by fluorescence of calcein, indicating a decrease of labile iron in the presence of fer-1. The notion of such as pseudo-catalytic cycle of the ferrostatin-iron complex was also investigated by means of quantum mechanics calculations, which confirmed the reduction of an alkoxyl radical model by fer-1 and the reduction of fer-1 radical by ferrous iron. In summary, GPx4 and fer-1 in the presence of ferrous iron, produces, by distinct mechanism, the most relevant anti-ferroptotic effect, i.e the disappearance of initiating lipid hydroperoxides

Nanotechnology in Tumor Biomarker Detection: The Potential of Liganded Nanoclusters as Nonlinear Optical Contrast Agents for Molecular Diagnostics of Cancer

Cancer is one of the leading causes of premature death, and, as such, it can be prevented by developing strategies for early and accurate diagnosis. Cancer diagnostics has evolved from the macroscopic detection of malignant tissues to the fine analysis of tumor biomarkers using personalized medicine approaches. Recently, various nanomaterials have been introduced into the molecular diagnostics of cancer. This has resulted in a number of tumor biomarkers that have been detected in vitro and in vivo using nanodevices and corresponding imaging techniques. Atomically precise ligand-protected noble metal quantum nanoclusters represent an interesting class of nanomaterials with a great potential for the detection of tumor biomarkers. They are characterized by high biocompatibility, low toxicity, and suitability for controlled functionalization with moieties specifically recognizing tumor biomarkers. Their non-linear optical properties are of particular importance as they enable the visualization of nanocluster-labeled tumor biomarkers using non-linear optical techniques such as two-photon-excited fluorescence and second harmonic generation. This article reviews liganded nanoclusters among the different nanomaterials used for molecular cancer diagnosis and the relevance of this new class of nanomaterials as non-linear optical probe and contrast agents.Project STIM-REI, European Regional Development Fund—the Operational Programme Competitiveness and Cohesion 2014–2020Peer Reviewe

High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress

Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workflow, including IgG enrichment, HILIC glycopeptide purification, and nano-LC-MS analysis of tryptic glycopeptides applied for the analysis of rat IgG. A cohort of 80 animals was exposed to seven stressors in a customized chronic stress protocol with blood and tissue sampling in three timepoints. Young female rats experienced an increase in agalactosylated glycoforms on IgG2a and IgG2c accompanied by a decrease in monogalactosylation. Among old females, increased galactosylation was observed in the IgG2b subclass, pointing to an anti-inflammatory activity of IgG. Additionally, IgG Fc N-glycosylation patterns in Sprague Dawley rats were analyzed, quantified, and reported for the first time. Our findings emphasize age-, sex- and subclass-dependent differences in IgG glycosylation related to chronic stress exposure, confirming the relevance of newly developed methods for further research in glycobiology of rodent immune response. SIGNIFICANCE: In this study, we showed that a high-throughput streamlined methodology based on protein L 96-well monolithic plates for efficient rat IgG immunoaffinity enrichment from blood plasma, paired with appropriate tryptic glycopeptide preparation, HILIC-SPE enrichment, and nano-LC-MS methods was suitable for quick processing of large sample sets. We report a subclass-specific profiling and changes in rat IgG Fc galactosylation and adrenal gland immunohistochemistry of male and female animals exposed to a customized chronic stress protocol

Plasma N-glycome composition associates with chronic low back pain

BACKGROUND: Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging. Moreover, symptoms and prognosis of LBP are influenced by age, gender, occupation, habits, and psychological factors. LBP may be characterized by an underlying inflammatory process. Previous studies indicated a connection between inflammatory response and total plasma N-glycosylation. We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease. METHODS: Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up. Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery. RESULTS: We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls. Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP. CONCLUSIONS: Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation. GENERAL SIGNIFICANCE: To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology

Koncert praizvedbi (studenti Muzičke akademije Sveučilišta u Zagrebu, 31. 5. 2022.)

Drugi dio snimke koncerta praizvedbi skladbi studenata Muzičke akademije Sveučilišta u Zagrebu. Koncert je održan na Muzičkoj akademiji u Koncertnoj dvorani "Blagoje Bersa" 31. 5. 2022. Izvođači: Doris Tkalčević (violina), Luka Jalšovec (klavir), Niko Kos (kontrabas), Svit Štarkl (udaraljke), Paula Bukvić (violina), Dora Šimunić (viola), Karla Kostović (violončelo), Matej Vukić (truba), Shushan Ghukasyan (klavir), Ana Labazan Brajša (violina), Marija Matshkalyan (violina), Tin Reba (viola), Janko Franković (violončelo), Mateo Narančić (klavir), Lovro Čutić (truba), Marko Mumlek (truba), Filip Pavlić (truba), Kristina Strinić (truba), Barbara Kšenek (flauta), Sunčica Hadaš (klarinet), Petar Krokar (klavir), Jana Palac (violina), Ema Perić (violina), Ana Majer (violončelo), Ante Labetić (violina), Filip Nemet Gužvić (viola), Bruno Tarbuk (viola), Zoran Resnik (violončelo), Damir Gregurić, v. umj. sur. (klavir), mješoviti zbor (soprani: Dora Ćutić, Vita Jozić, Latica Kovačević, Sara Vrdoljak, Antonija Žarković; alti: Karla Bakter, Katarina Boras, Lovorka Brkić, Valeria Markić, Gabriela Posavec, Marina Veža, Noemi Vučković; tenori: Lovro Bzdilik, Emanuel Tomljenović; baritoni: Lovro Ivić, Bruno Kota, Šime Vuksan; basovi: Mihael Horvat, Ivan Makar, Ronald Strabić). Program: 1. Tin Ujević: Prva studija za violinu (izvođač: Doris Tkalčević); 2. Luka Jalšovec: Chiaroscuro (izvođači: Luka Jalšovec, Niko Kos); 3. Svit Štarkl: Prelude (izvođač: Svit Štarkl); 4. Bruno Kota: Horloge za mješoviti zbor i gudački kvartet (izvođači: mješoviti zbor, Ante Labetić, Filip Nemet Gužvić, Bruno Tarbuk, Zoran Resnik); 5. Ivan Makar: Stavak za klavir (izvođač: Damir Gregurić, v. umj. sur.); 6. Karlo Bogatac: Nokturno (izvođači: Paula Bukvić, Doris Tkalčević, Dora Šimunić, Karla Kostović, Matej Vukić); 7. Fabijan Košćak: Les Episodes, kvintet za klavir i gudače (izvođači: Shushan Ghukasyan, Ana Labazan Brajša, Marija Matshkalyan, Tin Reba, Janko Franković); 8. Josip Prajz: Omne vivum ex vivo (izvođači: Mateo Narančić, Lovro Čutić, Marko Mumlek, Filip Pavlić, Kristina Strinić); 9. Fabijan Košćak: Tri večernje slike (izvođači: Barbara Kšenek, Sunčica Hadaš, Petar Krokar, Jana Palac, Ema Perić, Tin Reba, Ana Majer)

Koncert praizvedbi (studenti Muzičke akademije Sveučilišta u Zagrebu, 31. 5. 2022.)

Prvi dio snimke koncerta praizvedbi skladbi studenata Muzičke akademije Sveučilišta u Zagrebu. Koncert je održan na Muzičkoj akademiji u Koncertnoj dvorani "Blagoje Bersa" 31. 5. 2022. Izvođači: Doris Tkalčević (violina), Luka Jalšovec (klavir), Niko Kos (kontrabas), Svit Štarkl (udaraljke), Paula Bukvić (violina), Dora Šimunić (viola), Karla Kostović (violončelo), Matej Vukić (truba), Shushan Ghukasyan (klavir), Ana Labazan Brajša (violina), Marija Matshkalyan (violina), Tin Reba (viola), Janko Franković (violončelo), Mateo Narančić (klavir), Lovro Čutić (truba), Marko Mumlek (truba), Filip Pavlić (truba), Kristina Strinić (truba), Barbara Kšenek (flauta), Sunčica Hadaš (klarinet), Petar Krokar (klavir), Jana Palac (violina), Ema Perić (violina), Ana Majer (violončelo), Ante Labetić (violina), Filip Nemet Gužvić (viola), Bruno Tarbuk (viola), Zoran Resnik (violončelo), Damir Gregurić, v. umj. sur. (klavir), mješoviti zbor (soprani: Dora Ćutić, Vita Jozić, Latica Kovačević, Sara Vrdoljak, Antonija Žarković; alti: Karla Bakter, Katarina Boras, Lovorka Brkić, Valeria Markić, Gabriela Posavec, Marina Veža, Noemi Vučković; tenori: Lovro Bzdilik, Emanuel Tomljenović; baritoni: Lovro Ivić, Bruno Kota, Šime Vuksan; basovi: Mihael Horvat, Ivan Makar, Ronald Strabić). Program: 1. Tin Ujević: Prva studija za violinu (izvođač: Doris Tkalčević); 2. Luka Jalšovec: Chiaroscuro (izvođači: Luka Jalšovec, Niko Kos); 3. Svit Štarkl: Prelude (izvođač: Svit Štarkl); 4. Bruno Kota: Horloge za mješoviti zbor i gudački kvartet (izvođači: mješoviti zbor, Ante Labetić, Filip Nemet Gužvić, Bruno Tarbuk, Zoran Resnik); 5. Ivan Makar: Stavak za klavir (izvođač: Damir Gregurić, v. umj. sur.); 6. Karlo Bogatac: Nokturno (izvođači: Paula Bukvić, Doris Tkalčević, Dora Šimunić, Karla Kostović, Matej Vukić); 7. Fabijan Košćak: Les Episodes, kvintet za klavir i gudače (izvođači: Shushan Ghukasyan, Ana Labazan Brajša, Marija Matshkalyan, Tin Reba, Janko Franković); 8. Josip Prajz: Omne vivum ex vivo (izvođači: Mateo Narančić, Lovro Čutić, Marko Mumlek, Filip Pavlić, Kristina Strinić); 9. Fabijan Košćak: Tri večernje slike (izvođači: Barbara Kšenek, Sunčica Hadaš, Petar Krokar, Jana Palac, Ema Perić, Tin Reba, Ana Majer)

Koncert praizvedbi (studenti Muzičke akademije Sveučilišta u Zagrebu, 31. 5. 2022.)

Prvi dio snimke koncerta praizvedbi skladbi studenata Muzičke akademije Sveučilišta u Zagrebu. Koncert je održan na Muzičkoj akademiji u Koncertnoj dvorani "Blagoje Bersa" 31. 5. 2022. Izvođači: Doris Tkalčević (violina), Luka Jalšovec (klavir), Niko Kos (kontrabas), Svit Štarkl (udaraljke), Paula Bukvić (violina), Dora Šimunić (viola), Karla Kostović (violončelo), Matej Vukić (truba), Shushan Ghukasyan (klavir), Ana Labazan Brajša (violina), Marija Matshkalyan (violina), Tin Reba (viola), Janko Franković (violončelo), Mateo Narančić (klavir), Lovro Čutić (truba), Marko Mumlek (truba), Filip Pavlić (truba), Kristina Strinić (truba), Barbara Kšenek (flauta), Sunčica Hadaš (klarinet), Petar Krokar (klavir), Jana Palac (violina), Ema Perić (violina), Ana Majer (violončelo), Ante Labetić (violina), Filip Nemet Gužvić (viola), Bruno Tarbuk (viola), Zoran Resnik (violončelo), Damir Gregurić, v. umj. sur. (klavir), mješoviti zbor (soprani: Dora Ćutić, Vita Jozić, Latica Kovačević, Sara Vrdoljak, Antonija Žarković; alti: Karla Bakter, Katarina Boras, Lovorka Brkić, Valeria Markić, Gabriela Posavec, Marina Veža, Noemi Vučković; tenori: Lovro Bzdilik, Emanuel Tomljenović; baritoni: Lovro Ivić, Bruno Kota, Šime Vuksan; basovi: Mihael Horvat, Ivan Makar, Ronald Strabić). Program: 1. Tin Ujević: Prva studija za violinu (izvođač: Doris Tkalčević); 2. Luka Jalšovec: Chiaroscuro (izvođači: Luka Jalšovec, Niko Kos); 3. Svit Štarkl: Prelude (izvođač: Svit Štarkl); 4. Bruno Kota: Horloge za mješoviti zbor i gudački kvartet (izvođači: mješoviti zbor, Ante Labetić, Filip Nemet Gužvić, Bruno Tarbuk, Zoran Resnik); 5. Ivan Makar: Stavak za klavir (izvođač: Damir Gregurić, v. umj. sur.); 6. Karlo Bogatac: Nokturno (izvođači: Paula Bukvić, Doris Tkalčević, Dora Šimunić, Karla Kostović, Matej Vukić); 7. Fabijan Košćak: Les Episodes, kvintet za klavir i gudače (izvođači: Shushan Ghukasyan, Ana Labazan Brajša, Marija Matshkalyan, Tin Reba, Janko Franković); 8. Josip Prajz: Omne vivum ex vivo (izvođači: Mateo Narančić, Lovro Čutić, Marko Mumlek, Filip Pavlić, Kristina Strinić); 9. Fabijan Košćak: Tri večernje slike (izvođači: Barbara Kšenek, Sunčica Hadaš, Petar Krokar, Jana Palac, Ema Perić, Tin Reba, Ana Majer)

Koncert praizvedbi (studenti Muzičke akademije Sveučilišta u Zagrebu, 31. 5. 2022.)

Drugi dio snimke koncerta praizvedbi skladbi studenata Muzičke akademije Sveučilišta u Zagrebu. Koncert je održan na Muzičkoj akademiji u Koncertnoj dvorani "Blagoje Bersa" 31. 5. 2022. Izvođači: Doris Tkalčević (violina), Luka Jalšovec (klavir), Niko Kos (kontrabas), Svit Štarkl (udaraljke), Paula Bukvić (violina), Dora Šimunić (viola), Karla Kostović (violončelo), Matej Vukić (truba), Shushan Ghukasyan (klavir), Ana Labazan Brajša (violina), Marija Matshkalyan (violina), Tin Reba (viola), Janko Franković (violončelo), Mateo Narančić (klavir), Lovro Čutić (truba), Marko Mumlek (truba), Filip Pavlić (truba), Kristina Strinić (truba), Barbara Kšenek (flauta), Sunčica Hadaš (klarinet), Petar Krokar (klavir), Jana Palac (violina), Ema Perić (violina), Ana Majer (violončelo), Ante Labetić (violina), Filip Nemet Gužvić (viola), Bruno Tarbuk (viola), Zoran Resnik (violončelo), Damir Gregurić, v. umj. sur. (klavir), mješoviti zbor (soprani: Dora Ćutić, Vita Jozić, Latica Kovačević, Sara Vrdoljak, Antonija Žarković; alti: Karla Bakter, Katarina Boras, Lovorka Brkić, Valeria Markić, Gabriela Posavec, Marina Veža, Noemi Vučković; tenori: Lovro Bzdilik, Emanuel Tomljenović; baritoni: Lovro Ivić, Bruno Kota, Šime Vuksan; basovi: Mihael Horvat, Ivan Makar, Ronald Strabić). Program: 1. Tin Ujević: Prva studija za violinu (izvođač: Doris Tkalčević); 2. Luka Jalšovec: Chiaroscuro (izvođači: Luka Jalšovec, Niko Kos); 3. Svit Štarkl: Prelude (izvođač: Svit Štarkl); 4. Bruno Kota: Horloge za mješoviti zbor i gudački kvartet (izvođači: mješoviti zbor, Ante Labetić, Filip Nemet Gužvić, Bruno Tarbuk, Zoran Resnik); 5. Ivan Makar: Stavak za klavir (izvođač: Damir Gregurić, v. umj. sur.); 6. Karlo Bogatac: Nokturno (izvođači: Paula Bukvić, Doris Tkalčević, Dora Šimunić, Karla Kostović, Matej Vukić); 7. Fabijan Košćak: Les Episodes, kvintet za klavir i gudače (izvođači: Shushan Ghukasyan, Ana Labazan Brajša, Marija Matshkalyan, Tin Reba, Janko Franković); 8. Josip Prajz: Omne vivum ex vivo (izvođači: Mateo Narančić, Lovro Čutić, Marko Mumlek, Filip Pavlić, Kristina Strinić); 9. Fabijan Košćak: Tri večernje slike (izvođači: Barbara Kšenek, Sunčica Hadaš, Petar Krokar, Jana Palac, Ema Perić, Tin Reba, Ana Majer)