Biological Flora of Britain and Ireland: <i>Hammarbya paludosa</i>

1. This account presents information on all aspects of the biology of Hammarbya paludosa (L.) Kuntze (bog orchid, bog adder's-mouth orchid) (Malaxis paludosa (L.) Sw., Ophrys paludosa L.), that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the Biological Flora of Britain and Ireland: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history, and conservation.2. Hammarbya paludosa is a small bisexual perennial forb. It occurs in open habitats in bogs, mires and heaths as well as semi-shaded open woodland and, in some areas, in shaded coniferous forest. It is found in a few locations in the southern England (the New Forest, Dorset, Devon and Cornwall), in Cumbria and Northumberland, and in western counties in Wales. It is rare in Ireland but wide spread but very local in Scotland. H. paludosa has a Holarctic circumboreal range. Ninety per cent of known locations are recorded in Northern Europe and the British Isles.3. Hammarbya paludosa occurs on Sphagnum, other mosses and bare peat, overlying a variety of superficial deposits and sedimentary, metamorphic and igneous rocks. The pH of the rooting substrates ranges from acidic to neutral and slightly alkaline. Its nutrient status is oligotrophic, although occasionally mesotrophic, and very rarely eutrophic. It grows well in conditions of consistent lateral flow of water in the substratum.4. Hammarbya paludosay is visited by small insects of the order Diptera. Two species of gnats are recorded as orchid pollinators: Phronia digitata and Sciara thomae. It produces a small amount of nectar but is often considered as non-rewarding. The seeds are dust-like, dispersed by wind and water and the species has a short-term seed bank. Tiny bulbils (propagules) are formed on the leaf margins. They are dispersed by water.5. Hammarbya paludosa has declined in Britain since the late 19th Century primarily due to drainage of its mire habitats in lowland regions of southern and eastern England and Wales. The decline slowed down by the 2000s, and new locations have been found in Scotland and Ireland

Luminescence properties and time-resolved spectroscopy of rare-earth doped SrMoO4 single crystals

The work of V. Pankratova was supported by the financial support of Scientific Research Project for Students and Young Researchers (SJZ/2020/05) realized at Institute of Solid State Physics, University of Latvia. The Institute of Solid State Physics, University of Latvia as the Center of Excellence has received funding from the European Union’s Horizon 2020 Framework Programme H2020-WIDESPREAD-01-2016-2017-TeamingPhase2 under grant agreement No. 739508, project CAMART2.Luminescence properties of nominally pure and doped with Eu3+ and Pr3+ ions SrMoO4 single crystals grown by the Czochralski method have been studied. Thermal quenching of intrinsic emission of pure and doped SrMoO4 single crystals has been observed, as well as a correlation of thermal quenching activation energies with rare-earth ion concentration has been observed. Tunable laser was used to study time-resolved luminescence in a range from 10 K to room temperature. The effect of dopant nature and concentration on intrinsic emission and decay kinetics has been elucidated. --//-- Viktorija Pankratova, Elizaveta E. Dunaeva, Irina S. Voronina, Anna P. Kozlova, Roman Shendrik, Vladimir Pankratov, Luminescence properties and time-resolved spectroscopy of rare-earth doped SrMoO4 single crystals, Optical Materials: X, Volume 15, 2022, 100169, ISSN 2590-1478, https://doi.org/10.1016/j.omx.2022.100169. Article published under the CC BY-NC-ND licence.Scientific Research Project for Students and Young Researchers (SJZ/2020/05); the Institute of Solid State Physics, University of Latvia as the Center of Excellence has received funding from the European Union’s Horizon 2020 Framework Programme H2020-WIDESPREAD-01-2016-2017-TeamingPhase2 under grant agreement No. 739508, project CAMART2

PROFESSIONAL ORIENTATION COMPUTER TESTING AND COUNSELING STUDYING AS A TOOL FOR INNOVATION HUMAN POTENTIAL OF THE REGION

В статье рассмотрены вопросы применения профориентационного компьютерного тестирования и консультирования при профессиональном самоопределении школьников 8–11-х классов. В качестве средств самопознания и развития личности использовался компьютерный психодиагностический комплекс «Профориентатор». Результаты тестирования использовались в профориентационном консультировании респондентов. Полученные результаты могут являться ключевым моментом в плане построения жизненной траектории развития личности школьника. Для региона это может служить средством для инновационного развития кадрового потенциала.The article examines the application of professional orientation of computer testing and counseling for professional self-determination of students of 8–11 grades. As a means of self-knowledge and personal development to use computer psychodiagnostic complex “Proforientator”. The test results were used in vocational guidance counseling respondents. The results can be a key factor in terms of building the life path of the individual student. For the region, this can serve as a tool for innovative development of human resources

The impact of lifestyle factors on age-related differences in hair trace element content in pregnant women in the third trimester

BACKGROUND: Trace elements play a significant role in the regulation of human reproduction, while advanced age may have a significant impact on trace element metabolism. The objective of the present study was to assess the impact of lifestyle factors on age-related differences in hair trace element content in pregnant women in the third trimester. METHODS: A total of 124 pregnant women aged 20&ndash;29 (n = 72) and 30&ndash;39 (n = 52) were ex- amined. Scalp hair trace element content was assessed using inductively coupled plasma mass spectrometry at NexION 300D (Perkin Elmer, USA) after microwave digestion. RESULTS: The results showed that the elder pregnant women had 36% (p = 0.009), 14% (p = 0.045), and 45% (p = 0.044) lower hair Zn, V, and Cd content, and 16% (p = 0.044) higher hair B levels &ndash; in comparison to the respective younger group values. Multiple regression analysis demonstrated that the age of the women had a significant influence on hair V and Zn levels. B content was also significantly influenced by age at first intercourse, smoking status, and specific dietary habits. None of the lifestyle factors were associated with hair Cd content in pregnant women. Hair V levels were also affected by following a special diet. Interestingly, alcohol intake did not have a significant impact on hair trace element content. CONCLUSIONS: These data indicate that lifestyle factors have a significant influence on age-related changes in hair trace elements during pregnancy that may impact the outcome of pregnancy

Genomic features of resistant <i>Klebsiella pneumonia</i>, isolated from the bloodstream and cerebrospinal fluid of pediatric hospital patients

Introduction. Carbapenemase-producing Klebsiella pneumoniae (CP-Kp), which are international high-risk clones, have become a problem of utmost importance. CP-Kps, adapting to the hospital environment, evolve into convergent pathotypes. Such variants combine traits of two genetic lineages: multidrug resistant (MDR) and hypervirulent. The pathotypes, along with MDR K. pneumoniae, pose an exceptional threat to young patients during systemic infection. The objective of this study is the detailed molecular genetic analysis of MDR isolates of K. pneumoniae detected during the monitoring of resistant Gram-negative bacteria at the National Medical Research Center for Children’s Health in 2014–2021. Materials and methods. Whole-genome sequencing with a subsequent bioinformatics analysis of eight MDR isolates from the bloodstream and cerebrospinal fluid. Results. MDR isolates belonged to 4 sublineages (SL): SL307, SL395, SL29 and SL1198. In the genomes of 6 pangrug-resistant (PDR) isolates, genes associated with resistance to all categories of antibiotics recommended for Enterobacteriaceae therapy were identified. Plasmids were present in all genomes. In 6 isolates, plasmids contained heavy metal ion resistance operons in addition to antibiotic resistance genes. Prophages within the plasmids were also involved in the transfer of resistance genes. The ST395 isolate from the cerebrospinal fluid belonged to the convergent pathotype in terms of resistance and virulence. Comparison of genomes within SLs revealed recombination events in the K- and O-locus regions and the Yersiniabactin operon. Conclusion. Thus, in a sample of resistant K. pneumoniae isolated from bloodstream and cerebrospinal fluid, 6 PDR isolates were detected, one of which belongs to the convergent pathotype ST395

Design, synthesis, computational and biological evaluation of new anxiolytics

Abstract New anxiolytics have been discovered by prediction of biological activity with computer programs PASS and DE DEREK for a heterogeneous set of 5494 highly chemically diverse heterocyclic compounds (thiazoles, pyrazoles, isatins, a fused imidazoles and others). The majority of tested compounds exhibit the predicted anxiolytic effect. The most potent activity was found in 2 (4 nitro phenyl) 3 (4 phenylpiperazinomethyl)imidazo[1,2 a]pyridine 8, 1 [(4 bromophenyl) 2 oxoethyl] 3 (1,3 dioxolano) 2 indolinone 3, 5 hydroxy 3 methoxycarbonyl 1 phenylpyrazole 5 and 2 (4 fluorophenyl) 3 (4 methylpiperazinomethyl)imidazo[1,2 a]pyridine 7. The application of the computer assisted approach significantly reduced the number of synthesized and tested compounds and increased the chance of finding new chemical entities (NCEs)

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin