Evaluation of the urban design storm concept

This report describes an evaluation of the assumption commonly employed in drainage design that the return period of the rainfall used to design a system is the same as the peak flow produced by that rainfall. Specifically, the sensitivity of the frequency response of four catchments to design storm parameters is examined. Parameters include, hyetograph shape, antecedent soil moisture and rainfall duration. A continuous simulation model is used to compute simulated historical frequency responses for three different long term rainfall records. Design storms are also developed from depth-duration-frequency analyses of the rainfall data. Comparisons are made on frequency graphs. It is concluded that an appropriate choice of design storm parameters can produce a design which yields peak flows of the desired return period.U.S. Department of the InteriorU.S. Geological SurveyOpe

Rebates and Reward Programs: Conflicting Drivers

Rebate programs and customer reward programs have evolved almost side by side within the hospitality, tourism, services and retailing sectors. Interestingly, they both share a common theme of delaying rewards for consumers. In each case consumers are motivated to purchase a good or service contingent upon a reward that is delayed until a later time. At present there has been little research that examines how these programs function together and whether when implemented in tandem that they might actually be in conflict. An online survey was completed by 68 members of a shopping blog that asked about their participation and satisfaction with various rebate and reward programs. Data revealed a strong positive relationship between rebate proneness and various deal-seeking shopping behaviors, while a negative relationship was found between rebate usage and loyalty variables. We suggest that these parallel programs may actually be in conflict with each other and that practitioners must carefully manage these programs to avoid converting their loyal customers into deal seekers

Illinois least-cost sewer system design model: ILSD-1 & 2 user’s guide

ILSD models are sewer system models for least-cost optimal design of the entire system. ILSD-1 designs for a specified layout the size and slope of the sewers with or without detention storages with user supplied rainfall and/or inlet hydrographs. ILSD-2 is similar to ILSD-1 but also with risk consideration; i.e., with the risk damage cost included in the optimization procedure and a risk equation supplied by the user. The user may choose either ILSD-1 or 2 as he (she) wishes and according to the available data. This user's guide provides the necessary information to use the computer program. Data preparation for various options to fit different engineering situations is presented.U.S. Department of the InteriorU.S. Geological SurveyOpe

Electron monochromator mass spectrometry for the analysis of whole bacteria and bacterial spores. Anal. Chem

Spores from a variety of Bacillus species were analyzed with direct probe mass spectrometry using an electron monochromator to select electrons of distinct energies for ionization. Electron energies were chosen to match the electron capture energies of taxonomically important compounds such as dipicolinic acid and fatty acids. Previous negative ion interferences were not observed when the monochromator was used, and the signal-tonoise ratio of targeted compounds was significantly enhanced using this approach. To demonstrate the selectivity of the technique, the monochromator was swept over a range of electron energies while monitoring the masses of compounds with known electron capture energies. Scanning the monochromator while the mass spectrometer was operated in single-ion mode enabled dipicolinic acid to be detected in 10 5 spores. The results presented here demonstrate the utility of the electron monochromator for selectively ionizing compounds directly in bacteria and bacterial spores

Time and "angular" dependent backgrounds from stationary axisymmetric solutions

Backgrounds depending on time and on "angular" variable, namely polarized and unpolarized $S^1 \times S^2$ Gowdy models, are generated as the sector inside the horizons of the manifold corresponding to axisymmetric solutions. As is known, an analytical continuation of ordinary $D$-branes, $iD$-branes allows one to find $S$-brane solutions. Simple models have been constructed by means of analytic continuation of the Schwarzchild and the Kerr metrics. The possibility of studying the $i$-Gowdy models obtained here is outlined with an eye toward seeing if they could represent some kind of generalized $S$-branes depending not only on time but also on an ``angular'' variable.Comment: 24 pages, 5 figures, corrected typos, references adde

An Essential Role for the Proximal but Not the Distal Cytoplasmic Tail of Glycoprotein M in Murid Herpesvirus 4 Infection

Murid herpesvirus-4 (MuHV-4) provides a tractable model with which to define common, conserved features of gamma-herpesvirus biology. The multi-membrane spanning glycoprotein M (gM) is one of only 4 glycoproteins that are essential for MuHV-4 lytic replication. gM binds to gN and is thought to function mainly secondary envelopment and virion egress, for which several predicted trafficking motifs in its C-terminal cytoplasmic tail could be important. We tested the contribution of the gM cytoplasmic tail to MuHV-4 lytic replication by making recombinant viruses with varying C-terminal deletions. Removing an acidic cluster and a distal YXXΦ motif altered the capsid distribution somewhat in infected cells but had little effect on virus replication, either in vitro or in vivo. In contrast, removing a proximal YXXΦ motif as well completely prevented productive replication. gM was still expressed, but unlike its longer forms showed only limited colocalization with co-transfected gN, and in the context of whole virus appeared to support gN expression less well. We conclude that some elements of the gM cytoplasmic tail are dispensible for MuHV-4 replication, but the tail as a whole is not

Identification of 15 new psoriasis susceptibility loci highlights the role of innate immunity

To gain further insight into the genetic architecture of psoriasis, we conducted a meta-analysis of 3 genome-wide association studies (GWAS) and 2 independent data sets genotyped on the Immunochip, including 10,588 cases and 22,806 controls. We identified 15 new susceptibility loci, increasing to 36 the number associated with psoriasis in European individuals. We also identified, using conditional analyses, five independent signals within previously known loci. The newly identified loci shared with other autoimmune diseases include candidate genes with roles in regulating T-cell function (such as RUNX3, TAGAP and STAT3). Notably, they included candidate genes whose products are involved in innate host defense, including interferon-mediated antiviral responses (DDX58), macrophage activation (ZC3H12C) and nuclear factor (NF)-κB signaling (CARD14 and CARM1). These results portend a better understanding of shared and distinctive genetic determinants of immune-mediated inflammatory disorders and emphasize the importance of the skin in innate and acquired host defense

Genome-wide scan reveals association of psoriasis with IL-23 and NF-κB pathways

Psoriasis is a common immune-mediated disorder that affects the skin, nails and joints. To identify psoriasis susceptibility loci, we genotyped 438,670 SNPs in 1,409 psoriasis cases and 1,436 controls of European ancestry. We followed up 21 promising SNPs in 5,048 psoriasis cases and 5,041 controls. Our results provide strong support for the association of at least seven genetic loci and psoriasis (each with combined P less than 5 × 10−8). Loci with confirmed association include HLA-C, three genes involved in IL-23 signaling (IL23A, IL23R, IL12B), two genes that act downstream of TNF-α and regulate NF-κB signaling (TNIP1, TNFAIP3) and two genes involved in the modulation of Th2 immune responses (IL4, IL13). Although the proteins encoded in these loci are known to interact biologically, we found no evidence for epistasis between associated SNPs. Our results expand the catalog of genetic loci implicated in psoriasis susceptibility and suggest priority targets for study in other auto-immune disorders