Grapevine virus L: a novel vitivirus in grapevine

Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Soltero Brisbane, Reid. Foundation Plant Services; Estados UnidosFil: Voncina, Darko. University of Zagreb; CroaciaFil: Almeida, Rodrigo P.. University of California at Berkeley; Estados UnidosFil: Blouin, Arnaud G.. No especifíca;Fil: Al Rwahnih, Maher. University of California at Berkeley; Estados UnidosFil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentin

Mosquito Repellency of Polyester Nets Treated with Cyclodextrin/Repellent Complexes

Fabric treatments with β-Cyclodextrins (β-CD) have been studied for different applications as nanotechnologycal approaches to achieve functional textiles. In particular, repellents and insecticides have been incorporated in β-CD treated textiles to prolong their release. In this case, Citriodiol®, a naturally derived mosquito repellent, was incorporated to β-CD treated polyester (PET) nets. Two methods for citriodiol inclusion were studied; i) pipette dripping or ii) impregnation of fabric in a plastic bag, in order to increase the repellent activity of PET textile substrates. Release profiles were analyzed by gas chromatography and repellency was monitored by in vivo assays with Aedes aegypti mosquitoes. Long lasting and reloadable mosquito repellent nets could be achieved by treating PET knits with citriodiol/β-CD complexes.Centro de Estudios Parasitológicos y de Vectore

Mosquito Repellency of Polyester Nets Treated with Cyclodextrin/Repellent Complexes

Fabric treatments with β-Cyclodextrins (β-CD) have been studied for different applications as nanotechnologycal approaches to achieve functional textiles. In particular, repellents and insecticides have been incorporated in β-CD treated textiles to prolong their release. In this case, Citriodiol®, a naturally derived mosquito repellent, was incorporated to β-CD treated polyester (PET) nets. Two methods for citriodiol inclusion were studied; i) pipette dripping or ii) impregnation of fabric in a plastic bag, in order to increase the repellent activity of PET textile substrates. Release profiles were analyzed by gas chromatography and repellency was monitored by in vivo assays with Aedes aegypti mosquitoes. Long lasting and reloadable mosquito repellent nets could be achieved by treating PET knits with citriodiol/β-CD complexes.Centro de Estudios Parasitológicos y de Vectore

New distributional data on bryophytes of Poland and Slovakia, 13

This work presents a list of localities for the following species: Didymodon fallax var. brevifolius, Fissidens exilis, Nowellia curvifolia, Orthocaulis atlanticus, Orthotrichum lyellii, Ptilium crista-castrensis, and Syntrichia virescens

New distributional data on Bryophytes of Poland and Slovakia, 8

This work presents a list of localities for the following species: Anastrophyllum michauxii, Campylopus introflexus, Cephaloziella elachista, Cinclidotus fontinaloides, Cololejeunea calcarea, Dicranum viride, Didymodon spadiceus, Fissidens dubius var. mucronatus, Fossombronia wondraczekii, Fuscocephaloziopsis macrostachya, Hypnum cupressiforme var. subjulaceum, Lophozia ascendens, Mesoptychia heterocolpos, Nowellia curvifolia, Rhytidiadelphus loreus, Saccobasis polita, and Trichocolea tomentella

A contribution to the moss and liverwort flora of the Reberce Nature Reserve at the Przemyśl Foothills (Western Carpathians, Poland)

The paper presents the results of studies on bryophyte flora of the Reberce forest reserve located in the Przemyśl foothills. Thirty-three species of liverworts, 70 species and two varieties of mosses were found. Terrestrial (66 species) and epixylic (58) bryophytes formed the largest ecological groups in the reserve. Twenty-four protected species were also recorded, eight endangered bryophytes and six taxa considered as primeval forest species. The most interesting finds are: Bazzania trilobata, Buxbaumia viridis, Calypogeia suecica, Cephalozia catenulata, Frullania dilatata, Metzgeria conjugata, Nowellia curvifolia, Orthotrichum lyellii, O. stramineum, Trichocolea tomentella and Ulota crispa