Diabetic alteration versus postnatal maturation of rat kidney glycoconjugates — comparative detection by lectin probes

A panel of ten lectins with different carbohydrate specificities, including three original lectin preparations (MPFA, LABA, and LVA), was used for the investigation of rat kidney glycoconjugate remodeling during postnatal morphogenesis, and the findings were compared with the impairments seen in streptozotocin-induced diabetic nephropathy. Postnatal morphogenesis was accompanied by the accumulation and generalization of DMan, LFuc, and NeuNAc, with simultaneous reduction of DGal and DGalNAc sugar determinants and enhanced heterogeneity of renal microstructure. The most significant redistribution of lectin receptor sites was detected between postnatal days 1 and 20. Beginning from postnatal day 20, renal corpuscles showed selective MPFA, WGA, and RCA labeling. Stabilization of carbohydrate determinants on postnatal day 60 coincided with rat kidney maturity. Diabetic nephropathy induced carbohydrate remodeling reciprocal to that seen during postnatal development, that is, enhanced exposure of DGal and DGalNAc, reduced reactivity of DMan, LFuc, and NeuNAc determinants, and increased lectin labeling of renal tubule brush borders. These results extend the existing data on rearrangement of rat kidney glycoconjugates under physiological and pathological conditions, as well as demonstrate the applicability of three original lectin preparations in glycoconjugate histochemistry. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 92–102

INVESTIGATION OF THE CHEMICAL COMPOSITION AND EFFICIENCY OF SEED OIL OF SPINDLE TREE (EUONYMUS EUROPAEA L.) ON THE MODEL OF NON-ALLERGIC DERMATITIS

The aim of the research was to study the chemical composition and effectiveness of the seed oil of Spindle tree (Euonymus europaea L.) on the non-allergic contact dermatitis model. The oil was obtained by the extraction by petroleum ether from the seeds. The analysis of fatty acids and determination of their quantitative content was carried out using gas chromatography. Determination of carotenoids and tocopherols content in fatty oils was carried out after chromatography on a silica gel column. Investigation of anti-inflammatory action of the Spindle tree seed oil on white rats was carried. Determination of biochemical parameters of blood plasma was performed on the semi-automatic biochemical analyzer BS3000M (Poland). The yield of oil was 20–28 % of the weight of the seeds. Nine fatty acids were identified in the oil by the gas chromatography, among which 4 are unsaturated (oleic, palmitoleic, linoleic and linolenic), which together make up 87.79 % of all fatty acids from this oil. Spindle tree oil contains 26±5 mg – % carotenoids and 40±5 mg – % tocopherols, which were separated by chromatography on a silica gel column. There were no symptoms of intoxication with the introduction of Spindle tree oil in the stomach of rats and it can be classified in the 4 the grade of danger in accordance with GOST 12.1.005-88. The anti-inflammatory activity of Spindle tree oil in comparison with the oil of Sea buckthorn on the model of non-allergic contact dermatitis was weaker, but sufficient to recommend it for the treatment of skin diseases in ointments

INVESTIGATION OF THE CHEMICAL COMPOSITION AND EFFICIENCY OF SEED OIL OF SPINDLE TREE (EUONYMUS EUROPAEA L.) ON THE MODEL OF NON-ALLERGIC DERMATITIS

The aim of the research was to study the chemical composition and effectiveness of the seed oil of Spindle tree (Euonymus europaea L.) on the non-allergic contact dermatitis model.The oil was obtained by the extraction by petroleum ether from the seeds. The analysis of fatty acids and determination of their quantitative content was carried out using gas chromatography. Determination of carotenoids and tocopherols content in fatty oils was carried out after chromatography on a silica gel column. Investigation of anti-inflammatory action of the Spindle tree seed oil on white rats was carried. Determination of biochemical parameters of blood plasma was performed on the semi-automatic biochemical analyzer BS3000M (Poland).The yield of oil was 20–28 % of the weight of the seeds. Nine fatty acids were identified in the oil by the gas chromatography, among which 4 are unsaturated (oleic, palmitoleic, linoleic and linolenic), which together make up 87.79 % of all fatty acids from this oil. Spindle tree oil contains 26±5 mg – % carotenoids and 40±5 mg – % tocopherols, which were separated by chromatography on a silica gel column. There were no symptoms of intoxication with the introduction of Spindle tree oil in the stomach of rats and it can be classified in the 4 the grade of danger in accordance with GOST 12.1.005-88.The anti-inflammatory activity of Spindle tree oil in comparison with the oil of Sea buckthorn on the model of non-allergic contact dermatitis was weaker, but sufficient to recommend it for the treatment of skin diseases in ointments

Adaptation of children with the special necessities to the studies at elementary school

В статті розглянуто особливості адаптації дітей з особливими потребами до навчання в початковій школі. The article discusses the features adaptation of children with the special necessities to the studies at elementary school

Isolation and identification in human blood serum of the proteins possessing the ability to bind with 48 kDa form of unconventional myosin 1c and their possible diagnostic and prognostic value

We firstly identified 48 kDa molecular form of the unconventional myosin 1c (p48/Myo1C), and isolated it from blood serum of multiple sclerosis patients. The amount of p48/Myo1C in human blood serum correlated with some autoimmune, hemato-oncological and neurodegenerative diseases and thus may serve as a potential molecular biomarker. The biological functions of this protein in human blood remain unknown. Previously, we used the monodisperse magnetic poly (glycidyl methacrylate)(mag-PGMA–NH2) microspheres with immobilized 48/Myo1C and western-blot analysis, which allowed us to identify IgM and IgG immunoglobulins presenting an affinity to this protein. Here, we used mass spectrometry followed by the western blotting in order to identify other blood serum proteins with affinity to 48/Myo1C. The obtained data demonstrate that 48/Myo1C binds to component 3 of the complement and the antithrombin-III proteins. A combination of magnetic microparticle-based affinity chromatography with MALDI–TOF mass spectrometry and an in silico analysis provided an opportunity to identify the partners of interaction of 48/Myo1C with other proteins, in particular those participating in complement and coagulation cascades