Mitochondrial DNA haplotypes indicate two postglacial re-colonization routes of the spruce bark beetle Ips typographus through northern Europe to Scandinavia

Species in northern Europe re-colonized the region after the last glacial maximum via several routes, which could have lingering signatures in current intraspecific trait variation. The spruce bark beetle, Ips typographus, occurs across Europe, and biological differences have been found between southern and northern Scandinavian populations. However, the postglacial history of I. typographus in Scandinavia has not been previously studied at a fine geographical scale. Therefore, we collected specimens across northern Europe and analysed the genetic variation in a quite large mitochondrial fragment (698 bp). A high genetic diversity was found in some of the most northern populations, in the Baltic States, Gotland and central Europe. Detected genetic and phylogeographic structures suggest that I. typographus re-colonized Scandinavia via two pathways, one from the northeast and one from the south. These findings are consistent with the re-colonization history of its host plant, Picea abies. However, we observed low haplotype and nucleotide diversity in southern Scandinavian populations of I. typographus, indicating that (unlike P. abies) it did not disperse across the Baltic Sea in multiple events. Further, the divergence among Scandinavian populations was shallow, conflicting with a scenario where I. typographus expanded concurrently with its host plant from a 'cryptic refugium' in the northwest

Pioglitazone Prevents Capillary Rarefaction in Streptozotocin-Diabetic Rats Independently of Glucose Control and Vascular Endothelial Growth Factor Expression

Background/Aims: Reduction of capillary network density occurs early in the development of metabolic syndrome and may be relevant for the precipitation of diabetes. Agonists of the peroxisome proliferator-activated receptor (PPAR)-gamma transcription factor are vasculoprotective, but their capacity for structural preservation of the microcirculation is unclear. Methods: Male Wistar rats were rendered diabetic by streptozotocin and treated with pioglitazone in chow for up to 12 weeks. Capillary density was determined in heart and skeletal muscle after platelet endothelial cell adhesion molecule-1 (PECAM-1) immunostaining. Hallmarks of apoptosis and angiogenesis were determined. Results: Capillary density deteriorated progressively in the presence of hyperglycemia (from 971/mm(2) to 475/mm(2) in quadriceps muscle during 13 weeks). Pioglitazone did not influence plasma glucose, left ventricular weight, or body weight but nearly doubled absolute and relative capillary densities compared to untreated controls (1.2 vs. 0.6 capillaries/myocyte in heart and 1.5 vs. 0.9 capillaries/myocyte in quadriceps muscle) after 13 weeks of diabetes. No antiapoptotic or angiogenic influence of pioglitazone was detected while a reduced expression of hypoxia-inducible factor-3 alpha and PPAR coactivator-1 alpha (PGC-1 alpha) mRNA as well as vascular endothelial growth factor (VEGF) protein possibly occurred as a consequence of improved vascularization. Conclusion: Pioglitazone preserves microvascular structure in diabetes independently of improvements in glycemic control and by a mechanism unrelated to VEGF-mediated angiogenesis. Copyright (C) 2012 S. Karger AG, Base

Geochemical characterisation of northern Norwegian fjord surface sediments: a baseline for further paleo-environmental investigations

Norwegian fjord sediments are promising archives for very high resolution records of past environmental changes. Recent investigations of the modern depositional environment within fjords revealed that the accurate quantification of the inputs, sources, and sedimentary preservation of organic and inorganic material is crucial to decipher long term past climate signals in the sedimentary record of a certain fjord. Here, we investigate the elemental composition, bulk mineral assemblage and grain size distribution of forty-one surface sediment samples from a northern Norwegian fjord system. We reveal modern geochemical and sedimentological processes that occur within the Vestfjord, Ofotfjord and Tysfjord. Our results indicate a very heterogeneous sediment supply and intricate sedimentation processes. We propose that this is related to the complex fjord bathymetry, a low hydrodynamic energy environment, differences in the hinterland bedrock composition and a relatively small drainage area causing a rather diffuse freshwater inflow. Moreover, we show that marine carbonate productivity is the main calcite and Ca source in all three fjords

Two real parton contributions to non-singlet kernels for exclusive QCD DGLAP evolution

Results for the two real parton differential distributions needed for implementing a next-to-leading order (NLO) parton shower Monte Carlo are presented. They are also integrated over the phase space in order to provide solid numerical control of the MC codes and for the discussion of the differences between the standard $\bar{MS}$ factorization and Monte Carlo implementation at the level of inclusive NLO evolution kernels. Presented results cover the class of non-singlet diagrams entering into NLO kernels. The classic work of Curci-Furmanski-Pertonzio was used as a guide in the calculations.Comment: 34 pages, 3 figure

Parton Fragmentation within an Identified Jet at NNLL

The fragmentation of a light parton i to a jet containing a light energetic hadron h, where the momentum fraction of this hadron as well as the invariant mass of the jet is measured, is described by "fragmenting jet functions". We calculate the one-loop matching coefficients J_{ij} that relate the fragmenting jet functions G_i^h to the standard, unpolarized fragmentation functions D_j^h for quark and gluon jets. We perform this calculation using various IR regulators and show explicitly how the IR divergences cancel in the matching. We derive the relationship between the coefficients J_{ij} and the quark and gluon jet functions. This provides a cross-check of our results. As an application we study the process e+ e- to X pi+ on the Upsilon(4S) resonance where we measure the momentum fraction of the pi+ and restrict to the dijet limit by imposing a cut on thrust T. In our analysis we sum the logarithms of tau=1-T in the cross section to next-to-next-to-leading-logarithmic accuracy (NNLL). We find that including contributions up to NNLL (or NLO) can have a large impact on extracting fragmentation functions from e+ e- to dijet + h.Comment: expanded introduction, typos fixed, journal versio

Sorting of chromosomes by magnetic separation

Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension. Streptavidin was covalently coupled to the surface of magnetic beads and these were incubated with the hybridized chromosomes. The human chromosomes were bound to the magnetic beads through the strong biotin-streptavidin complex and then rapidly separated from nonlabeled Chinese hamster chromosomes by a simple permanent magnet. The hybridization was visualized by additional binding of avidin-FITC (fluorescein) to the unoccupied biotinylated human DNA bound to the human chromosomes. After magnetic separation, up to 98% of the individual chromosomes attached to magnetic beads were classified as human chromosomes by fluorescence microscopy

The Soft-Collinear Bootstrap: N=4 Yang-Mills Amplitudes at Six and Seven Loops

Infrared divergences in scattering amplitudes arise when a loop momentum $\ell$ becomes collinear with a massless external momentum $p$. In gauge theories, it is known that the L-loop logarithm of a planar amplitude has much softer infrared singularities than the L-loop amplitude itself. We argue that planar amplitudes in N=4 super-Yang-Mills theory enjoy softer than expected behavior as $\ell \parallel p$ already at the level of the integrand. Moreover, we conjecture that the four-point integrand can be uniquely determined, to any loop-order, by imposing the correct soft-behavior of the logarithm together with dual conformal invariance and dihedral symmetry. We use these simple criteria to determine explicit formulae for the four-point integrand through seven-loops, finding perfect agreement with previously known results through five-loops. As an input to this calculation we enumerate all four-point dual conformally invariant (DCI) integrands through seven-loops, an analysis which is aided by several graph-theoretic theorems we prove about general DCI integrands at arbitrary loop-order. The six- and seven-loop amplitudes receive non-zero contributions from 229 and 1873 individual DCI diagrams respectively.Comment: 27 pages, 48 figures, detailed results including PDF and Mathematica files available at http://goo.gl/qIKe8 v2: minor corrections v3: figure 7 corrected, Lemma 2 remove

Split tendon transfers for the correction of spastic varus foot deformity: a case series study

<p>Abstract</p> <p>Background</p> <p>Overactivity of anterior and/or posterior tibial tendon may be a causative factor of spastic varus foot deformity. The prevalence of their dysfunction has been reported with not well defined results. Although gait analysis and dynamic electromyography provide useful information for the assessment of the patients, they are not available in every hospital. The purpose of the current study is to identify the causative muscle producing the deformity and apply the most suitable technique for its correction.</p> <p>Methods</p> <p>We retrospectively evaluated 48 consecutive ambulant patients (52 feet) with spastic paralysis due to cerebral palsy. The average age at the time of the operation was 12,4 yrs (9-18) and the mean follow-up 7,8 yrs (4-14). Eigtheen feet presented equinus hind foot deformity due to gastrocnemius and soleus shortening. According to the deformity, the feet were divided in two groups (Group I with forefoot and midfoot inversion and Group II with hindfoot varus). The deformities were flexible in all cases in both groups. Split anterior tibial tendon transfer (SPLATT) was performed in Group I (11 feet), while split posterior tibial tendon transfer (SPOTT) was performed in Group II (38 feet). In 3 feet both procedures were performed. Achilles tendon sliding lengthening (Hoke procedure) was done in 18 feet either preoperatively or concomitantly with the index procedure.</p> <p>Results</p> <p>The results in Group I, were rated according to Hoffer's clinical criteria as excellent in 8 feet and satisfactory in 3, while in Group II according to Kling's clinical criteria were rated as excellent in 20 feet, good in 14 and poor in 4. The feet with poor results presented residual varus deformity due to intraoperative technical errors.</p> <p>Conclusion</p> <p>Overactivity of the anterior tibial tendon produces inversion most prominent in the forefoot and midfoot and similarly overactivity of the posterior tibial tendon produces hindfoot varus. The deformity can be clinically unidentifiable in some cases when Achilles shortening co-exists producing foot equinus. By identifying the muscle causing the deformity and performing the appropriate technique, very satisfying results were achieved in the majority of our cases. In three feet both muscles contributed to a combined deformity and simultaneous SPLATT and SPOTT were considered necessary. For complex foot deformities where the component of cavus co-exists, supplementary procedures are required along with the index operation to obtain the best result.</p

Impact-parameter dependent nuclear parton distribution functions: EPS09s and EKS98s and their applications in nuclear hard processes

We determine the spatial (impact parameter) dependence of nuclear parton distribution functions (nPDFs) using the $A$-dependence of the spatially independent (averaged) global fits EPS09 and EKS98. We work under the assumption that the spatial dependence can be formulated as a power series of the nuclear thickness functions $T_A$. To reproduce the $A$-dependence over the entire $x$ range we need terms up to $[T_A]^4$. As an outcome, we release two sets, EPS09s (LO, NLO, error sets) and EKS98s, of spatially dependent nPDFs for public use. We also discuss the implementation of these into the existing calculations. With our results, the centrality dependence of nuclear hard-process observables can be studied consistently with the globally fitted nPDFs for the first time. As an application, we first calculate the LO nuclear modification factor $R^{1jet}_{AA}$ for primary partonic-jet production in different centrality classes in Au+Au collisions at RHIC and Pb+Pb collisions at LHC. Also the corresponding central-to-peripheral ratios $R_{CP}^{1jet}$ are studied. We also calculate the LO and NLO nuclear modification factors for single inclusive neutral pion production, $R_{dAu}^{\pi^0}$, at mid- and forward rapidities in different centrality classes in d+Au collisions at RHIC. In particular, we show that our results are compatible with the PHENIX mid-rapidity data within the overall normalization uncertainties given by the experiment. Finally, we show our predictions for the corresponding modifications $R_{pPb}^{\pi^0}$ in the forthcoming p+Pb collisions at LHC.Comment: 36 page