Influence of hyperthermal regimes on experimental teratoma development in vitro

We screened for the impact of hyperthermal regimes varying in the cumulative equivalent minutes at 43°C (CEM43°C) and media composition on tumour development using an original teratoma in vitro model. Rat embryos (three germ layers) were microsurgically isolated and cultivated at the air-liquid interface. During a two week period, ectodermal, mesodermal and endodermal derivatives developed within trilaminar teratomas. Controls were grown at 37°C. Overall growth was measured, and teratoma survival and differentiation were histologically assessed. Cell proliferation was stereologically quantified by the volume density of Proliferating Cell Nuclear Antigen. Hyperthermia of 42°C, applied for 15 minutes after plating (CEM43°C 3.75 minutes), diminished cell proliferation (P ˂ .0001) and enhanced differentiation of both myotubes (P ˂ .01) and cylindrical epithelium (P ˂ .05). Hyperthermia of 43°C applied each day for 30 minutes during the first week (CEM43°C 210 minutes) impaired overall growth (P ˂ .01) and diminished cell proliferation (P ˂ .0001). Long-term hyperthermia of 40.5°C applied for two weeks (CEM43°C 630 minutes) significantly impaired survival (P ˂ .005). Long-term hyperthermia of 40.5°C applied from the second day when differentiation of tissues begins (CEM43°C 585 minutes) impaired survival (P ˂ .0001), overall growth (P ˂ .01) and cartilage differentiation (P ˂ .05). No teratomas survived extreme regimes: 43°C for 24 hours (CEM43°C 1440 minutes), hyperthermia in the scant serum-free medium (CEM43°C 630 minutes) or treatment with an anti-HSP70 antibody before long-term hyperthermia 40.5°C from the second day (CEM43°C 585 minutes). This in vitro research provided novel insights into the impact of hyperthermia on the development of experimental teratomas from their undifferentiated sources and are thus of potential interest for future therapeutic strategies in corresponding in vivo models

Acute hemorrhagic edema of infancy – is it really a mild, benign disease?

Background. Acute hemorrhagic edema of infancy (AHEI) is a rare vasculitis, which presents dramatically as palpable purpuric skin lesions on the limb, face and auricles, with swelling of these parts and low-grade fever, in children up to 2 years of age. To date, approximately 400 cases have been described in the literature. The etiology remains mostly unknown. With or without treatment, AHEI goes to spontaneous recovery within 1-3 weeks, usually without any complications. To our knowledge, compartment syndrome as complication of AHEI has only been reported in one case. We present an unusual case of AHEI with serious complications due to compartment syndrome of the right-hand fingers. Case. A 16–month–old male child presented with fever and sudden appearance and rapid spread of palpable, painless, non–itching ecchymotic hematomas on the thigh, cheeks, earlobes, forearms, dorsum of hands and feet, with mild edema of these regions. Complete systemic examination and all vital parameters were normal for age. There was no history of bleeding disorders in the family. Except low hemoglobin on complete blood count and increased D-dimer values, all other laboratory investigations were in the normal range. Changes on the right forearm and hand expanded on almost the entire dorsal side and all surfaces of the fingers, with pronounced swelling and formation of bullous lesions, which were spreading and cracking. Skin biopsy confirmed nonspecific small–vessel vasculitis. That required the use of Methylprednisolone, low-molecular-weight heparin, antibiotics and debridement of necrotic eschar, with necrectomy of the affected fingers. Conclusions. Early recognition of AHEI is important to avoid unnecessary investigation and therapy. On the other hand, our reported case warns that unexpected complications may occur

Crustal velocity structure associated with the eastern Tennessee seismic zone: Vp and Vs images based upon local earthquake tomography

We present three-dimensional P and S wave velocity models for the active eastern Tennessee seismic zone (ETSZ) using arrival time data from more than 1000 local earthquakes. A nonlinear tomography method is used that involves sequential inversion for model and hypocenter parameters. We image several velocity anomalies that persist through most of the inversion volume. Some anomalies support the presence of known features such as an ancient rift zone in northern Tennessee. Other anomalies reveal the presence of basement features that can be correlated with regional gravity and magnetic anomalies. We image a narrow, NE-SW trending, steeply dipping zone of low velocities that extends to a depth of at least 24 km and is associated with the vertical projection of the prominent New York-Alabama magnetic lineament. The low-velocity zone may have an apparent dip to the SE at depths exceeding 15 km. The low-velocity zone is interpreted as a major basement fault juxtaposing Granite-Rhyolite basement to the NW from Grenville southern Appalachian basement to the SE. Relocated hypocenters align in near-vertical segments suggesting reactivation of a distributed zone of deformation associated with a major strike-slip fault. We suggest that the ETSZ represents reactivation of an ancient shear zone established during formation of the super continent Rodinia. ©2013. American Geophysical Union. All Rights Reserved

RECURRENT WOMEN'S GENITAL CANDIDOSIS - HUMORAL IMMUNITY

The first explorations of the women's vaginal mucosa were devoted to thehumoral immunity study. There are few studies conceming the role of specific serumand secretion immunoglobulins in women with recurrent genital candidosis (RGC).The aim of the paper was to detect the antiCandida TgG and IgA antibodies in theexamined woman's blood in order to determine a possible causal relation between the findings of the specific immunoglobulin and those of the fungi of the Candida sort inthe woman's genital tract. The examined test group for the serological analyzescomprised 60 women with the RGC, namely, those having a positive finding of theCandida albicans in their vaginal secretion. The control group consisted of 60 chosenwomen with no finding of the Candida sp. infection/colonization of the genital tract.The specific antiCandida IgG and IgA antibodies in the examineđ women's bloodwere detennined by a non standard zed indirect immunofluorescent test. The specificanti Candida IgG antibodies were confirmed in 90% of the women with the RGC andin 45% of the control group women. The IgA specific immunoglobulins were foundin 12 women of the test group and only in one woman of the control group. The lowertiter of the IgG antibodies was proved in approximately the same number of womenof both the examined groups. A higher titer of the IgG antibodies was found in aconsiderably greater number of women with chronic fungi genital infection (34) withrespect to the control group women (9)

PET with 62Cu-ATSM and 62Cu-PTSM is a useful imaging tool for hypoxia and perfusion in pulmonary lesions.

ObjectiveHypoxia is a characteristic of many tumors and portends a worse prognosis in lung, cervical, prostate, and rectal cancers. Unlike the others, lung cancers present a unique challenge in measuring hypoxia, with invasive biopsies and higher rates of complications. Noninvasive imaging studies detecting hypoxia using isotopes of copper-diacetyl-bis(N4-methylthiosemicarbazone) ((62)Cu-ATSM) have predicted prognosis and treatment outcomes in some small feasibility trials. These images, however, may not identify all areas of hypoxia. Hence, we hypothesize that the addition of another PET imaging agent, copper-pyruvaldehyde-bis(N4-methylthiosemicarbazone) ((62)Cu-PTSM), which can detect areas of perfusion, can augment the information obtained in (62)Cu-ATSM PET scans.Subjects and methodsTo characterize tumors on the basis of both perfusion and hypoxia, 10 patients were studied using both (62)Cu-ATSM and (62)Cu-PTSM PET scans. In addition, proteomic arrays looking at specific proangiogenic, survival, and proinflammatory targets were assessed.ResultsSix of 10 patients had evaluable PET scans. Our initial experience of characterizing lung tumor hypoxia using (62)Cu-ATSM and (62)Cu-PTSM PET scans showed that visualization of areas with hypoxia normalized for perfusion is feasible. All studied tumors exhibited some hypoxia. Despite the small sample size, a positive relationship was noted between epidermal growth factor levels and (62)Cu-ATSM-detected hypoxia.ConclusionThis initial series of (62)Cu-ATSM and (62)Cu-PTSM PET scans shows that evaluating lung masses by visualizing hypoxia and perfusion is a feasible and novel technique to provide more information. Further investigation is warranted to assess the potential role of (62)Cu-ATSM and (62)Cu-PTSM PET techniques combined with proteomics as alternatives to invasive biopsy techniques in clinical care