Table1_Intranasal neuropeptide Y is most effective in some aspects of acute stress compared to melatonin, oxytocin and orexin.pdf

Objectives: In the current study, we compared the effects of a single intranasal administration of clomipramine with effects of four neuropeptides, melatonin, oxytocin, orexin, and neuropeptide Y, to compare them in an acute stress model.Methods: The anti-stress effect was evaluated in the sucrose preference and forced swimming tests. Serum corticosterone level in rats was measured to evaluate the stress response.Results: Neuropeptide Y reduced immobilization time in the Porsolt test and decreased corticosterone levels, but increased the anhedonia. Orexin had no positive effect on animal behavior, but decreased corticosterone levels. Oxytocin decreased immobilization time, maintained anhedonia at the level of control, but did not affect corticosterone levels. Melatonin demonstrated no positive effects in any of the tests.Conclusion: The intranasal administered neuropeptide Y could be a promising compound for the treatment of stress disorders.</p

Presentation1_Intranasal neuropeptide Y is most effective in some aspects of acute stress compared to melatonin, oxytocin and orexin.pdf

Objectives: In the current study, we compared the effects of a single intranasal administration of clomipramine with effects of four neuropeptides, melatonin, oxytocin, orexin, and neuropeptide Y, to compare them in an acute stress model.Methods: The anti-stress effect was evaluated in the sucrose preference and forced swimming tests. Serum corticosterone level in rats was measured to evaluate the stress response.Results: Neuropeptide Y reduced immobilization time in the Porsolt test and decreased corticosterone levels, but increased the anhedonia. Orexin had no positive effect on animal behavior, but decreased corticosterone levels. Oxytocin decreased immobilization time, maintained anhedonia at the level of control, but did not affect corticosterone levels. Melatonin demonstrated no positive effects in any of the tests.Conclusion: The intranasal administered neuropeptide Y could be a promising compound for the treatment of stress disorders.</p

Additional file 1: Table S1. of Low-dose lipopolysaccharide (LPS) inhibits aggressive and augments depressive behaviours in a chronic mild stress model in mice

Primer sequences for qPCR. Primers were custom designed and validated by PrimerDesign Ltd. (Southampton, UK). Figure S1. The effect of a low dose of LPS on locomotor activity at 24 and 48 h post-challenge in naïve mice. Naïve animals were subjected to a single dose of LPS (0.1 or 0.5 mg/kg) or vehicle injection and were tested at 24 or 48 h post-injection. (A) Neither the resting time was unaltered by the treatment in the TruScan open field nor (B) rearing in the novel cage test for the total number of rear. (C–E) Aggressive behaviour was also unaltered. Data are mean ± SEM, two-way ANOVA throughout. Figure S2. (A, B) Body weight in the chronic stress experiment. Experimental groups were balanced upon baseline mean values of body weight measured 7 days prior the start of the chronic stress experiment and LPS challenge. Mice exposed to chronic stress had a significant reduction in body weight as compared with baseline measurements (*p < 0.05, pairwise t test). Chronically stressed mice injected either with vehicle or LPS had similar mean body weight prior the LPS challenge. (C–E) Sucrose preference. Experimental groups were balanced upon baseline mean values of sucrose preference when evaluated 7 days prior the experiment chronic stress procedure and LPS challenge. Experimental groups had similar mean measures of sucrose and water intake. (p > 0.05, one-way ANOVA and post hoc Tukey test; see the text). (F) Naïve and stressed animals (10 days) were challenged with a single dose of LPS (0.1 mg/kg) or vehicle (saline) and tested 24 h thereafter in a novel cage test for total number of rears (see the text). Data are mean ± SEM. No differences between the groups were observed. Figure S3. (A–C) Baseline behaviour in a resident-intruder test. Experimental groups were balanced upon baseline mean scores of behaviours in a resident-intruder test that were studied 7 days prior the experimental chronic stress procedure and LPS challenge. Mice had similar mean measures of (A) latency to attack, (B) number of attack and (C )duration of crawl over behaviour. (p > 0.05, one-way ANOVA and post hoc Tukey test; see the text). (D) The latency to attack after the chronic stress was not significantly altered

Second temple and Roman period in historical work of Abraham ibn Daud

The thesis contains a Czech translation of Hebrew historiographical monographs Zikhron divre Romi and Divre Malkhe Yisra'el be-Bayit sheni by the Jewish historian Abraham ibn Daud (ca 1110 - ca 1180). The translation is accompanied by a commentary and an introduction divided into three chapters

MOESM9 of Temozolomide promotes genomic and phenotypic changes in glioblastoma cells

Additional file 9: Figure S1. qRT-PCR analysis of selected genes in TMZ-treated cells. a The relative gene expression ratios (fold-change) of stem cell markers CD133, OCT4, SOX2, and NANOG. b The relative gene expression ratio (fold-change) of MGMT. Gene expression fold-change values were derived by 2-ΔΔCT method (expressed as ratios relative to control values after normalization to the internal control TBP). TBP – TATA-binding protein; CD133 – prominin 1; OCT4 – POU class 5 homeobox 1; SOX2 – SRY (sex determining region Y)-box 2; NANOG – Nanog homeobox; MGMT – O-6-methylguanine-DNA methyltransferase

MOESM8 of Temozolomide promotes genomic and phenotypic changes in glioblastoma cells

Additional file 8: Table S8. Table S8. A list of genes/proteins, which were experimentally shown to increase/reduce the sensitivity of tumour cells to temozolomide (TMZ) treatment

MOESM5 of Temozolomide promotes genomic and phenotypic changes in glioblastoma cells

Additional file 5: Table S5. Table S5. A list of CCAs/NCCAs and their copy number variation in C6, C6TMZ, C6R1, and C6R2TMZ cells

MOESM3 of Temozolomide promotes genomic and phenotypic changes in glioblastoma cells

Additional file 3: Table S3. Table S3. A list of CCAs/NCCAs and their copy number variation in T98G and T98GTMZ cells

MOESM7 of Temozolomide promotes genomic and phenotypic changes in glioblastoma cells

Additional file 7: Table S7. Table S7. A list of annotated genes in gained 4p16.1-q26 region retrieved using a the DAVID bioinformatics resource or b the NCBI Map Viewer. These lists of annotated genes were cross-checked with a manually curated list of published proteins/miRNAs that were shown to contribute to TMZ resistance (see Suppl. Table S8), and hits were marked with colour

Methodological aspects of MRI of transplanted superparamagnetic iron oxide-labeled mesenchymal stem cells in live rat brain

<div><p><i>In vivo</i> tracking of transplanted mesenchymal stem cells (MSCs) migration and homing is vital for understanding the mechanisms of beneficial effects of MSCs transplantation in animal models of diseases and in clinical trials. Transplanted cells can be labeled with superparamagnetic iron oxide (SPIO) particles and visualized in vivo using a number of iron sensitive MRI techniques. However, the applicability of those techniques for SPIO-labeled MSCs tracking in live brain has not been sufficiently investigated. The goal of this study was to estimate the efficiency of various MRI techniques of SPIO-labeled cell tracing in the brain. To achieve that goal, the precision and specificity of T2WI, T2*WI and SWI (Susceptibility-Weighted Imaging) techniques of SPIO-labeled MSCs tracing <i>in vitro</i> and in live rat brain were for the first time compared in the same experiment. We have shown that SWI presents the most sensitive pulse sequence for SPIO-labeled MSCs MR visualization. After intracerebral administration due to limitations caused by local micro-hemorrhages the visualization threshold was 10² cells, while after intra-arterial transplantation SWI permitted detection of several cells or even single cells. There is just one publication claiming detection of individual SPIO-labeled MSCs in live brain, while the other state much lower sensitivity, describe detection of different cell types or high resolution tracing of MSCs in other tissues. This study confirms the possibility of single cell tracing in live brain and outlines the necessary conditions. SWI is a method convenient for the detection of single SPIO labeled MSCs and small groups of SPIO labeled MSCs in brain tissue and can be appropriate for monitoring migration and homing of transplanted cells in basic and translational neuroscience.</p></div