Clinical case: Mayer-Rokitansky-Kuster-Hauser syndrome, atypic form

Secţia Urologie IMSP SCM „Sf. Treime”, Catedra Urologie şi Nefrologie Chirurgicală, USMF „N. Testemiţanu”, Al V-lea Congres de Urologie, Dializă şi Transplant Renal din Republica Moldova cu participare internaţională (1-13 iunie 2011)Summary The Mayer-Rokitansky-kuster-Hauser (MRkH) syndrome is a rare anomaly in the prenatal development of the female genital tract. In this paper we report a clinical case of this syndrome observed in its atypical form with affected urogenital system, as well as uterine and renal agenesia. This case is interesting, as the patient did not receive any specific treatment associated with the syndrome until the age of 29. We provide a detailed description of the clinical case, its specifics and anamnesis. A literature review on the prenatal development of the female genital tract, urogenital system and the renal agenesia is also given

Гигиенические и эргономические аспекты, характерные для швейной промышленности

CMP Soroca, CSP Fălești, CSP Floreşti, CSP Orhei, CSP Chişinău, CSP Ialoveni, Conferinţa știinţifico-practică naţională cu participare internaţională Sănătatea ocupaţională: probleme și realizări prima ediţie 5-7 iunie 2014The paper presents some aspects hygienic ergonomic characteristic entities in the clothing industry and their impact on the health of workers exposed.В статье представлены некоторые гигиенические и эргономические аспекты, характерные для предприятий швейной промышленности и их влияние на здоровье работников, подвергающихся их воздействию

Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset]

Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles (“SmartFlares”) can result in ring like shaped GNP arrangements around the cell nucleus. Transmission electron microscopy revealed GNP accumulation in vicinity to the intracellular membrane structures including them of the endoplasmatic reticulum. A quantification of the radio therapeutic dose enhancement as a proof of principle was conducted with γH2AX foci analysis: The application of both – SmartFlares and unmodified GNPs – lead to a significant dose enhancement with a factor of up to 1.2 times the dose deposition compared to non-treated breast cancer cells. This enhancement effect was even more pronounced for SmartFlares. Furthermore, it was shown that a magnetic field of 1 Tesla simultaneously applied during irradiation has no detectable influence on neither the structure nor the dose enhancement dealt by gold nanoparticles

Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells.

Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles ("SmartFlares") can result in ring like shaped GNP arrangements around the cell nucleus. Transmission electron microscopy revealed GNP accumulation in vicinity to the intracellular membrane structures including them of the endoplasmatic reticulum. A quantification of the radio therapeutic dose enhancement as a proof of principle was conducted with γH2AX foci analysis: The application of both-SmartFlares and unmodified GNPs-lead to a significant dose enhancement with a factor of up to 1.2 times the dose deposition compared to non-treated breast cancer cells. This enhancement effect was even more pronounced for SmartFlares. Furthermore, it was shown that a magnetic field of 1 Tesla simultaneously applied during irradiation has no detectable influence on neither the structure nor the dose enhancement dealt by gold nanoparticles

Epifluorescence image of SkBr3 cells transfected with SmartFlares illuminated with a 671 nm laser.

<p>While Cy5 signals can be clearly seen in the cytoplasm of the cells, cells treated with no or unmodified GNP are almost not visible due to the very low auto-fluorescence at 671 nm (not shown). The scale bar equals 20 μm.</p

3D image of fluorescently labelled γH2AX foci (pink) in a SkBr3 cell nucleus after irradiation of 4 Gy and incubation with 8 μl SmartFlare solution.

<p>The foci are represented with NIS-Elements. The cube’s edge length equals 20 μm. No special foci arrangement or distribution could be detected, independently from dose or GNP concentration.</p

Localization microscopy images of a SkBr3 cells.

<p>Illumination at 561 nm transfected with 8 μl SmartFlare solution after 18 h incubation time with the characteristic ring like shape (A-C). Wide-field overview (A) and localization microscopy image (B) with points representing the loci of blinking events. Merged image (C) of wide-field overview (red) and detected GNP signals (yellow) of the pointillist localization microscopy image. Localization microscopy image of a cell treated by unmodified GNP for comparison (D). The scale bars equal 20 μm each.</p

Localization microscopy signal counts for unmodified GNP, SmartFlares.

<p>For each wavelength of 491 nm and 561 nm standard deviation and the error of the mean are given.</p

Analysis of foci volume and arrangement within the cell nucleus from top line to bottom line: Foci count of the fourth experiment analyzed with NIS elements; average membrane distance of the foci in [%] compared to the maximum possible radius within the nucleus; average foci volume in arbitrary units; volume ratio of all combined foci to the nucleus volume; note that the two values in brackets faced a very high standard deviation and are—Due to the low foci count—Not used for any conclusion even though fitting well to the other values.

<p>Analysis of foci volume and arrangement within the cell nucleus from top line to bottom line: Foci count of the fourth experiment analyzed with NIS elements; average membrane distance of the foci in [%] compared to the maximum possible radius within the nucleus; average foci volume in arbitrary units; volume ratio of all combined foci to the nucleus volume; note that the two values in brackets faced a very high standard deviation and are—Due to the low foci count—Not used for any conclusion even though fitting well to the other values.</p

Comparison of the averaged detected foci count for control (orange), unmodified GNP (green) and SmartFlare probes (brown) at 0 Gy, 2 Gy and 4 Gy.

<p>The standard deviation is highlighted as black arrow line. A significance level of 0.05 was used for all statistical tests. In <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0190183#pone.0190183.t002" target="_blank">Table 2</a> (below) the significance levels are presented which would be required that the significance disappears. These values have to be seen in relation to 0.05 if one compares the histograms in this figure.</p