Reconstruction of Model Defects of the Orbit of Laboratory Animals with Use of Resorbabl 3d Plates

В работе исследованы резорбируемые полимерные 3D-пластины на основе полиэфира гидроксимасляной кислоты – поли-3-гидроксибутирата (П(3ГБ)) в сравнении с коммерческим препаратом ООО “Пластика” (аллохрящ) для реконструкции дефекта нижней стенки глазной орбиты кроликов. Показано, что исследуемые 3D-пластины не вызывают воспалительной реакции после имплантации, способны выполнять функцию изолирующих элементов и обладают свойствами остеопротекции, способствуют сокращению сроков восстановления травмированной костной ткани и полному закрытию дефекта за 60 днейThe study investigated the resorbable polymeric 3D plates based on polyesters of hydroxybutyric acid – poly-3-hydroxybutyrate (P(3HB)) in comparison with the commercial preparation Ltd. “Plastic” (allocartilage) for regeneration of bottom wall orbit defect of rabbits. The studied 3D plates don’t induce inflammatory reactions after implantation. They can perform function of insulating elements and have osteoprotective properties; they contribute to shortening of the period of injured bone tissue restoration, and complete closure of defect for 60 day

High-Throughput Small-Molecule Crystallography at the ‘Belok’ Beamline of the Kurchatov Synchrotron Radiation Source: Transition Metal Complexes with Azomethine Ligands as a Case Study

This paper concisely describes capabilities of the ‘Belok’ beamline at the Kurchatov synchrotron radiation source, related to high-throughput small-molecule X-ray crystallography. As case examples, a series of four novel transition metal complexes with azomethine ligands were selected. The complexes demonstrate somewhat unexpected changes in the coordination geometry and nuclearity in response to the introduction of substituents in the ligand’s periphery

Reconstruction of Model Defects of the Orbit of Laboratory Animals with Use of Resorbabl 3d Plates

В работе исследованы резорбируемые полимерные 3D-пластины на основе полиэфира гидроксимасляной кислоты – поли-3-гидроксибутирата (П(3ГБ)) в сравнении с коммерческим препаратом ООО “Пластика” (аллохрящ) для реконструкции дефекта нижней стенки глазной орбиты кроликов. Показано, что исследуемые 3D-пластины не вызывают воспалительной реакции после имплантации, способны выполнять функцию изолирующих элементов и обладают свойствами остеопротекции, способствуют сокращению сроков восстановления травмированной костной ткани и полному закрытию дефекта за 60 днейThe study investigated the resorbable polymeric 3D plates based on polyesters of hydroxybutyric acid – poly-3-hydroxybutyrate (P(3HB)) in comparison with the commercial preparation Ltd. “Plastic” (allocartilage) for regeneration of bottom wall orbit defect of rabbits. The studied 3D plates don’t induce inflammatory reactions after implantation. They can perform function of insulating elements and have osteoprotective properties; they contribute to shortening of the period of injured bone tissue restoration, and complete closure of defect for 60 day

Chalcogenide Thin Films—Holographic Media for Augmented Reality Devices

Holographic characteristics of chalcogenide film materials with a typical thickness of 0.5–1.5 µm of a number of Ge-S and As-S compositions have been studied theoretically and experimentally with the aim of using them in augmented reality waveguide devices. The possibility of controlling the spectral and holographic properties of materials by varying their composition is shown. The recording of both volume and surface holographic elements operating in the total internal reflection mode was carried out. The operation of holograms in the “periscope mode” with a diffraction efficiency of about 10% is demonstrated. It is concluded that the recording of high-performance holographic elements on chalcogenide films requires significant values of exposure energy when recording in the near UV range (325–355 nm), which makes holographic recording currently ineffective for creating such elements

Synthesis, Structure, Spectral-Luminescent Properties, and Biological Activity of Chlorine-Substituted <i>N</i>-[2-(Phenyliminomethyl)phenyl]-4-methylbenzenesulfamide and Their Zinc(II) Complexes

New azomethine compounds of 2-(N-tosylamino)benzaldehyde or 5-chloro-2-(N-tosylamino)benzaldehyde and the corresponding chlorine-substituted anilines, zinc(II) complexes based on them have been synthesized. The structures of azomethines and their complexes were determined by elemental analysis, IR, 1H NMR, X-ray spectroscopy, and X-ray diffraction. It is found that all ZnL2 complexes have a tetrahedral structure according to XAFS and X-ray diffraction data. The photoluminescent properties of azomethines and zinc complexes in methylene chloride solution and in solid form have been studied. It is shown that the photoluminescence quantum yields of solid samples of the complexes are an order of magnitude higher compared to the solutions and range from 11.34% to 48.3%. The thermal properties of Zn(II) complexes were determined by thermal gravimetric analysis (TGA) and differential scanning calorimetry. The TGA curves of all the compounds suggest their high thermal stability up to temperatures higher than 290 °C. The electrochemical properties of all complexes were investigated by the cyclic voltammetry method. The multilayered devices ITO/PEDOT:PSS/NPD/Zn complex/ TPBI/LiF/Al with wide electroluminescence (EL) color range spanning the range from bluish-green (494 nm) to green (533 nm) and the high values of brightness, current and power efficiency were fabricated. The biological activity of azomethines and zinc complexes has been studied. In the case of complexes, the protistocidal activity of the zinc complex with azomethine of 5-chloro-2-(N-tosylamino)benzaldehyde with 4-chloroaniline was two times higher than the activity of the reference drug toltrazuril

Mononuclear Heptacoordinated 3d-Metal Helicates as a New Family of Single Ion Magnets

The series of Co(II), Fe(II), and Ni(II) mononuclear coordination compounds of [CoL(NCS)2]·3DMSO (1), [CoL(H2O)2](ClO4)2·DMSO (2), [CoL(H2O)(EtOH)][CoCl4]·2H2O (2a), [FeL(NCS)2]·DMSO (3), and [NiL(NCS)2]·CH3CN (4) composition (where L is 2,6-bis(1-(2-(4,6-dimethylpyrimidin-2-yl)hydrazineylidene)ethyl)pyridine), with an [MLA2] coordination unit (where A is a pair of apical monodentate ligands), was synthesized. In compounds 1, 2, 2a, and 3, the ligand L is pentadentate, and cobalt and iron ions are placed in a heavily distorted pentagonal pyramidal coordination environment, while in 4 the Ni(II) ion is hexacoordinated. Easy plane-type magnetic anisotropy (D = 13.69, 11.46, 19.5, and 6.2 cm−1 for 1, 2, 2a, and 4, respectively) was established for cobalt and nickel compounds, while easy axis-type magnetic anisotropy (D = −14.5 cm−1) was established for iron compound 3. The cobalt coordination compounds 1 and 2 show SIM behavior under a 1500 Oe external magnetic field, with effective magnetization reversal barriers of 65(1) and 60(1) K for 1 and 2, respectively. The combination of Orbach and Raman relaxation mechanisms was shown to adequately describe the temperature dependence of relaxation times for 1 and 2. CASSCF/NEVPT2 calculations were performed to model the parameters of the effective spin Hamiltonian for the compounds under study

Novel Genetically Encoded Bright Positive Calcium Indicator NCaMP7 Based on the mNeonGreen Fluorescent Protein

Green fluorescent genetically encoded calcium indicators (GECIs) are the most popular tool for visualization of calcium dynamics in vivo. However, most of them are based on the EGFP protein and have similar molecular brightnesses. The NTnC indicator, which is composed of the mNeonGreen fluorescent protein with the insertion of troponin C, has higher brightness as compared to EGFP-based GECIs, but shows a limited inverted response with an ΔF/F of 1. By insertion of a calmodulin/M13-peptide pair into the mNeonGreen protein, we developed a green GECI called NCaMP7. In vitro, NCaMP7 showed positive response with an ΔF/F of 27 and high affinity (Kd of 125 nM) to calcium ions. NCaMP7 demonstrated a 1.7-fold higher brightness and similar calcium-association/dissociation dynamics compared to the standard GCaMP6s GECI in vitro. According to fluorescence recovery after photobleaching (FRAP) experiments, the NCaMP7 design partially prevented interactions of NCaMP7 with the intracellular environment. The NCaMP7 crystal structure was obtained at 1.75 Å resolution to uncover the molecular basis of its calcium ions sensitivity. The NCaMP7 indicator retained a high and fast response when expressed in cultured HeLa and neuronal cells. Finally, we successfully utilized the NCaMP7 indicator for in vivo visualization of grating-evoked and place-dependent neuronal activity in the visual cortex and the hippocampus of mice using a two-photon microscope and an NVista miniscope, respectively