Mutations in the tryptophan operon allow PurF-independent thiamine synthesis by altering flux in vivo

Phosphoribosyl amine (PRA) is an intermediate in purine biosynthesis and also required for thiamine biosynthesis in Salmonella enterica. PRA is normally synthesized by phosphoribosyl pyrophosphate (PRPP) amidotransferase, a high-turnover enzyme of the purine biosynthetic pathway encoded by purF. However, PurF-independent PRA synthesis has been observed in strains with different genetic backgrounds and growing under diverse conditions. Genetic analysis has shown that the anthranilate synthase-phosphoribosyltransferase (AS-PRT) enzyme complex, involved in the synthesis of tryptophan, can play a role in the synthesis of phosphoribosyl amine (PRA). This work describes the in vitro synthesis of PRA in the presence of the purified components of AS-PRT complex. Results from in vitro assays and in vivo studies indicate the cellular accumulation of phosphoribosyl anthranilate can result in non-enzymatic PRA formation sufficient for thiamine synthesis. These studies have uncovered a mechanism used by cells to redistribute metabolites to ensure thiamine synthesis, and may define a general paradigm of metabolic robustness

Characterization of soils in an area of prescribed fire

The prescribed fire is a technique that is often used, it has several advantages. Pedological and hydropedological techniques were tested to assess the prescribed fire changes may cause in soils. This work was performed in Tresminas area (Vila Pouca de Aguiar, Northern Portugal), during February and March 2011. In the present study we applied several techniques. For the field sampling was followed the ISO 10381-1[1], ISO 10381-2[2], and FAO rules [3], as well as were used a grid with 17 points for measuring the soil parameters. During the fire, we have tried to check, with the assistance of the Portuguese Forestry Authority, some important parameters such as, the propagation speed, the size of the flame front and the intensity of energy emitted per unit area. Before the fire, was collected carefully soil disturbed and undisturbed samples for laboratory analysis, and measured soil water content; we also have placed four sets of thermocouples for measuring soil temperature. After the fire, were collected the thermocouples and new soil samples; the water content were measured in the soil and collected ashes. In the laboratory, after preparing and sieving the samples, were determined the soil particle size. The soil pH and electrical conductivity in water was also determined. The total carbon (TC) and inorganic carbon (IC)[4] was measured by a Shimadzu TOC-Vcsn. The water content in soil has not varied significantly before and after the fire, as well as soil pH and soil electrical conductivity. The TC and IC did not change, which was expected, since the fire not overcome the 200° C. Through the various parameters, we determined that the prescribed fire didn’t affect the soil. The low temperature of the fire and its rapid implementation that lead to the possible adverse effects caused by the wild fire didn’t occurred

Genomic characterization of murine monocytes reveals C/EBPβ transcription factor dependence of Ly6C(-) cells

Monocytes are circulating, short-lived mononuclear phagocytes, which in mice and man comprise two main subpopulations. Murine Ly6C(+) monocytes display developmental plasticity and are recruited to complement tissue-resident macrophages and dendritic cells on demand. Murine vascular Ly6C(-) monocytes patrol the endothelium, act as scavengers, and support vessel wall repair. Here we characterized population and single cell transcriptomes, as well as enhancer and promoter landscapes of the murine monocyte compartment. Single cell RNA-seq and transplantation experiments confirmed homeostatic default differentiation of Ly6C(+) into Ly6C(-) monocytes. The main two subsets were homogeneous, but linked by a more heterogeneous differentiation intermediate. We show that monocyte differentiation occurred through de novo enhancer establishment and activation of pre-established (poised) enhancers. Generation of Ly6C(-) monocytes involved induction of the transcription factor C/EBP{beta} and C/EBP{beta}-deficient mice lacked Ly6C(-) monocytes. Mechanistically, C/EBP{beta} bound the Nr4a1 promoter and controlled expression of this established monocyte survival factor

Spitzer Space Telescope study of disks in the young σ\sigma Orionis cluster

We report new Spitzer Space Telescope observations from the IRAC and MIPS instruments of the young (~ 3 Myr) sigma Orionis cluster. We identify 336 stars as members of the cluster using optical and near-infrared color magnitude diagrams. Using the spectral energy distribution (SED) slopes in the IRAC spectral range, we place objects in several classes: non-excess stars, stars with optically thick disks(like classical T Tauri stars), class I (protostellar) candidates, and stars with ``evolved disks''; the last exhibit smaller IRAC excesses than optically thick disk systems. In general, this classification agrees with the location expected in IRAC-MIPS color-color diagrams for these objects. We find that the evolved disk systems are mostly a combination of objects with optically thick but non-flared disks, suggesting grain growth and/or settling, and transition disks, systems in which the inner disk is partially or fully cleared of small dust. In all, we identify 7 transition disk candidates and 3 possible debris disk systems. As in other young stellar populations, the fraction of disks depends on the stellar mass, ranging from ~10% for stars in the Herbig Ae/Be mass range (>2 msun) to ~35% in the T Tauri mass range (1-0.1 msun). We find that the disk fraction does not decrease significantly toward the brown dwarf candidates (<0.1 msun). The IRAC infrared excesses found in stellar clusters and associations with and without central high mass stars are similar, suggesting that external photoevaporation is not very important in many clusters. Finally, we find no correlation between the X-ray luminosity and the disk infrared excess, suggesting that the X-rays are not strongly affected by disk accretion.Comment: 44pages, 17 figures. Sent to Ap

Leishmania spp. epidemiology of canine leishmaniasis in the Yucatan Peninsula

Canine Leishmaniasis is widespread in various Mexican states, where different species of Leishmania have been isolated from dogs. In the present study, we describe the detection of L. braziliensis, L. infantum, and L. mexicana in serum of dogs from the states of Yucatan and Quintana Roo in the Yucatan Peninsula (Mexico). A total of 412 sera were analyzed by ELISA using the total extract of the parasite and the iron superoxide dismutase excreted by different trypanosomatids as antigens. We found the prevalence of L. braziliensis to be 7.52%, L. infantum to be 6.07%, and L. mexicana to be 20.63%, in the dog population studied. The results obtained with ELISA using iron superoxide dismutase as the antigen were confirmed by western blot analysis with its greater sensitivity, and the agreement between the two techniques was very high

Gut microbiome variation modulates the effects of dietary fiber on host metabolism

Background: There is general consensus that consumption of dietary fermentable fiber improves cardiometabolic health, in part by promoting mutualistic microbes and by increasing production of beneficial metabolites in the distal gut. However, human studies have reported variations in the observed benefits among individuals consuming the same fiber. Several factors likely contribute to this variation, including host genetic and gut microbial differences. We hypothesized that gut microbial metabolism of dietary fiber represents an important and differential factor that modulates how dietary fiber impacts the host. Results: We examined genetically identical gnotobiotic mice harboring two distinct complex gut microbial communities and exposed to four isocaloric diets, each containing different fibers: (i) cellulose, (ii) inulin, (iii) pectin, (iv) a mix of 5 fermentable fibers (assorted fiber). Gut microbiome analysis showed that each transplanted community preserved a core of common taxa across diets that differentiated it from the other community, but there were variations in richness and bacterial taxa abundance within each community among the different diet treatments. Host epigenetic, transcriptional, and metabolomic analyses revealed diet-directed differences between animals colonized with the two communities, including variation in amino acids and lipid pathways that were associated with divergent health outcomes. Conclusion: This study demonstrates that interindividual variation in the gut microbiome is causally linked to differential effects of dietary fiber on host metabolic phenotypes and suggests that a one-fits-all fiber supplementation approach to promote health is unlikely to elicit consistent effects across individuals. Overall, the presented results underscore the importance of microbe-diet interactions on host metabolism and suggest that gut microbes modulate dietary fiber efficacy. [MediaObject not available: see fulltext.]Fil: Murga Garrido, Sofia M.. Universidad Nacional Autónoma de México; México. University of Wisconsin; Estados UnidosFil: Hong, Qilin. University of Wisconsin; Estados UnidosFil: Cross, Tzu Wen L.. University of Wisconsin; Estados Unidos. Purdue University; Estados UnidosFil: Hutchison, Evan R.. University of Wisconsin; Estados UnidosFil: Han, Jessica. Wisconsin Institute for Discovery; Estados UnidosFil: Thomas, Sydney P.. Wisconsin Institute for Discovery; Estados UnidosFil: Vivas, Eugenio I.. University of Wisconsin; Estados UnidosFil: Denu, John. Wisconsin Institute for Discovery; Estados UnidosFil: Ceschin, Danilo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; ArgentinaFil: Tang, Zheng Zheng. University of Wisconsin; Estados Unidos. Wisconsin Institute for Discovery; Estados UnidosFil: Rey, Federico E.. University of Wisconsin; Estados Unido

25 Orionis: A Kinematically Distinct 10 Myr Old Group in Orion OB1a

We report here on the photometric and kinematic properties of a well defined group of nearly 200 low-mass pre-main sequence stars, concentrated within ~ 1 deg of the early-B star 25 Ori, in the Orion OB1a sub-association. We refer to this stellar aggregate as the 25 Orionis group. The group also harbors the Herbig Ae/Be star V346 Ori and a dozen other early type stars with photometry, parallaxes, and some with IR excess emission, consistent with group membership. The number of high and low-mass stars is in agreement with expectations from a standard Initial Mass Function. The velocity distribution for the young stars in 25 Ori shows a narrow peak centered at 19.7 km/s, very close to the velocity of the star 25 Ori. Our results provide new and compelling evidence that the 25 Ori group is a distinct kinematic entity, and that considerable space and velocity structure is present in the Ori OB1a sub-association. The low-mass members follow a well defined band in the color-magnitude diagram, consistent with an isochronal age of ~ 7-10 Myr, depending on the assumed evolutionary model. The highest density of members is located near the star 25 Ori, but the actual extent of the cluster cannot be well constrained with our present data. In a simple-minded kinematic evolution scenario, the 25 Ori group may represent the evolved counterpart of a younger aggregate like the sigma Ori cluster. The 25 Ori stellar aggregate is the most populous ~ 10 Myr sample yet known within 500 pc, setting it as an excellent laboratory to study the evolution of solar-like stars and protoplanetary disks.Comment: 28 pages, 5 figures. Astrophysical Journal, in press. Abridged abstrac

A Complete Spectroscopic Survey of the Milky Way Satellite Segue 1: The Darkest Galaxy

We present the results of a comprehensive Keck/DEIMOS spectroscopic survey of the ultra-faint Milky Way satellite galaxy Segue 1. We have obtained velocity measurements for 98.2% of the stars within 67 pc (10 arcmin, or 2.3 half-light radii) of the center of Segue 1 that have colors and magnitudes consistent with membership, down to a magnitude limit of r=21.7. Based on photometric, kinematic, and metallicity information, we identify 71 stars as probable Segue 1 members, including some as far out as 87 pc. After correcting for the influence of binary stars using repeated velocity measurements, we determine a velocity dispersion of 3.7^{+1.4}_{-1.1} km/s, with a corresponding mass within the half-light radius of 5.8^{+8.2}_{-3.1} x 10^5 Msun. The stellar kinematics of Segue 1 require very high mass-to-light ratios unless the system is far from dynamical equilibrium, even if the period distribution of unresolved binary stars is skewed toward implausibly short periods. With a total luminosity less than that of a single bright red giant and a V-band mass-to-light ratio of 3400 Msun/Lsun, Segue 1 is the darkest galaxy currently known. We critically re-examine recent claims that Segue 1 is a tidally disrupting star cluster and that kinematic samples are contaminated by the Sagittarius stream. The extremely low metallicities ([Fe/H] < -3) of two Segue 1 stars and the large metallicity spread among the members demonstrate conclusively that Segue 1 is a dwarf galaxy, and we find no evidence in favor of tidal effects. We also show that contamination by the Sagittarius stream has been overestimated. Segue 1 has the highest measured dark matter density of any known galaxy and will therefore be a prime testing ground for dark matter physics and galaxy formation on small scales.Comment: 24 pages, 4 tables, 11 figures (10 in color). Submitted for publication in ApJ. V3 revised according to comments from the refere