Essential Oils of Plants as Biocides againstMicroorganisms Isolated from Cuban and Argentine Documentary Heritage

Fil: Borrego, Sofía. Laboratory of Preventive Conservation. National Archive of the Republic of Cuba. Havana; CubaFil: Valdés, Oderlaise. Laboratorio de Conservación Preventiva. Archivo Nacional de la República de Cuba. La Habana Vieja. La Habana; CubaFil: Vivar, Isbel. Laboratorio de Conservación Preventiva. Archivo Nacional de la República de Cuba. La Habana Vieja. La Habana; CubaFil: Lavin, Paola. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA). Departamento de Química. Facultad de Ciencias Exactas. Universidad Nacional de La Plata; ArgentinaFil: Gómez de Saravia, Sandra Gabriela. Cátedra de Zoología Invertebrados I. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Borges, Pedro. Food Industry Research Institute. Havana; Cub

Biocidal activity of two essential oils on fungi that cause degradation of paper documents

The aim of this study was to evaluate the biocidal activity of essential oils of Syzygium aromaticum (L.) Merr. and L. M. Perry (nail) and Allium sativum L. (garlic) against different fungal species producing paper degradation and deterioration. Essential oils (EOs) were obtained from harvested plants in their natural habitat in Cuba, and were tested against the species Aspergillus niger, Aspergillus flavus and Penicillium sp. which were isolated from archival indoor environments and documents with patrimonial value The biocidal activity was studied at different concentrations (70, 50, 25, 12.5 and 7.5%) using the agar diffusion method. The effect of extracts on paper alterations was studied through different techniques including determination of pH and number of copper and scanning electron microscopy (SEM) observations. EOs were analyzed by gas chromatography coupled to mass spectroscopy (GC/MS). The determination of inhibition zones by the agar diffusion method of the tested EOs showed a moderate and/or positive effect. The study of the antifungal activity on paper (“in vivo”) shows that both clove and garlic oils were potent biocides. Although the paper structure was not affected by EOs pure, some molecular damages were observed at lower concentrations across determinations of the pH and copper number.Facultad de Ciencias Exacta

Biocidal activity of two essential oils on fungi that cause degradation of paper documents

The aim of this study was to evaluate the biocidal activity of essential oils of Syzygium aromaticum (L.) Merr. and L. M. Perry (nail) and Allium sativum L. (garlic) against different fungal species producing paper degradation and deterioration. Essential oils (EOs) were obtained from harvested plants in their natural habitat in Cuba, and were tested against the species Aspergillus niger, Aspergillus flavus and Penicillium sp. which were isolated from archival indoor environments and documents with patrimonial value The biocidal activity was studied at different concentrations (70, 50, 25, 12.5 and 7.5%) using the agar diffusion method. The effect of extracts on paper alterations was studied through different techniques including determination of pH and number of copper and scanning electron microscopy (SEM) observations. EOs were analyzed by gas chromatography coupled to mass spectroscopy (GC/MS). The determination of inhibition zones by the agar diffusion method of the tested EOs showed a moderate and/or positive effect. The study of the antifungal activity on paper (“in vivo”) shows that both clove and garlic oils were potent biocides. Although the paper structure was not affected by EOs pure, some molecular damages were observed at lower concentrations across determinations of the pH and copper number.Facultad de Ciencias Exacta

Biocidal activity of two essential oils on fungi that cause degradation of paper documents

The aim of this study was to evaluate the biocidal activity of essential oils of Syzygium  aromaticum (L.) Merr. and L. M. Perry (nail) and Allium sativum L. (garlic) against different fungal species producing paper degradation and deterioration. Essential oils (EOs) were obtained from harvested plants in their natural habitat in Cuba, and were tested against the species Aspergillus niger, Aspergillus flavus and Penicillium sp. which were isolated from archival indoor environments and documents with patrimonial value The biocidal activity was studied at different concentrations (70, 50, 25, 12.5 and 7.5%) using the agar diffusion method. The effect of extracts on paper alterations was studied through different techniques including determination of pH and number of copper and scanning electron microscopy (SEM) observations. EOs were analyzed by gas chromatography coupled to mass spectroscopy (GC/MS). The determination of inhibition zones by the agar diffusion method of the tested EOs showed a moderate and/or positive effect. The study of the antifungal activity on paper (?in vivo?) shows that both clove and garlic oils were potent biocides. Although the paper structure was not affected by EOs pure, some molecular damages were observed at lower concentrations across determinations of the pH and copper number.Fil: Borrego, Sofía B. Archivo Nacional de Cuba Arnac; CubaFil: Gómez de Saravia, Sandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas. Área de Pinturas Protectoras | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Área de Pinturas Protectoras; ArgentinaFil: Valdés, Oderlaise. Archivo Nacional de Cuba Arnac; CubaFil: Vivar, Isbel. Archivo Nacional de Cuba Arnac; CubaFil: Battistoni, Patricia Adriana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Guiamet, Patricia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentin

Productos ambientalmente amigables de origen vegetal empleados en el control de microorganismos intervinientes en el biodeterioro del patrimonio cultural

Fil: Gómez de Saravia, Sandra Gabriela. Cátedra de Zoología Invertebrados I. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Borrego, Sofía. Laboratory of Preventive Conservation. National Archive of the Republic of Cuba. Havana; CubaFil: Lavin, Paola. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA). Departamento de Química. Facultad de Ciencias Exactas. Universidad Nacional de La Plata; ArgentinaFil: Valdés, Oderlaise. Laboratorio de Conservación Preventiva. Archivo Nacional de la República de Cuba. La Habana Vieja. La Habana; CubaFil: Vivar, Isbel. Laboratorio de Conservación Preventiva. Archivo Nacional de la República de Cuba. La Habana Vieja. La Habana; CubaFil: Battistoni, Patricia A.. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA). Departamento de Química. Facultad de Ciencias Exactas. Universidad Nacional de La Plata; ArgentinaFil: Guiamet, Patricia Sandra. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA). Departamento de Química. Facultad de Ciencias Exactas. Universidad Nacional de La Plata; Argentin

Environmentally friendly products from plants in the control of biodeteriogen agents

Fil: Gómez de Saravia, Sandra Gabriela. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA). Departamento de Química. Facultad de Ciencias Exactas. Universidad Nacional de La Plata; ArgentinaFil: Borrego, Sofía. Laboratorio de Conservación Preventiva. Archivo Nacional de la República de Cuba. La Habana Vieja; CubaFil: Lavin, Paola. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA). Departamento de Química. Facultad de Ciencias Exactas. Universidad Nacional de La Plata; ArgentinaFil: Valdés, Oderlaise. Laboratorio de Conservación Preventiva. Archivo Nacional de la República de Cuba. La Habana Vieja. La Habana; CubaFil: Vivar, Isbel. Laboratorio de Conservación Preventiva. Archivo Nacional de la República de Cuba. La Habana Vieja. La Habana; CubaFil: Battistoni, Patricia A.. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA). Departamento de Química. Facultad de Ciencias Exactas. Universidad Nacional de La Plata; ArgentinaFil: Guiamet, Patricia Sandra. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA). Departamento de Química. Facultad de Ciencias Exactas. Universidad Nacional de La Plata; Argentin

Essential Oils of Plants as Biocides against Microorganisms Isolated from Cuban and Argentine Documentary Heritage

Natural products obtained from plants with biocidal activity represent an alternative and useful source in the control of biodeterioration of documentary heritage, without negative environmental and human impacts. In this work, we studied the antimicrobial activity of seven essential oils against microorganisms associated with the biodeterioration of documentary heritage. The essential oils were obtained by steam distillation. The antimicrobial activity was analyzed using the agar diffusion method against 4 strains of fungi and 6 bacterial strains isolated fromrepositories air and documents of theNational Archive of the Republic of Cuba and theHistorical Archive of theMuseum of La Plata, Argentina. Anise and garlic oils showed the best antifungal activity at all concentrations studied, while oregano oil not only was effective against fungi tested but also prevented sporulation of them all. Orange sweet and laurel oils were ineffective against fungi. Clove, garlic, and oregano oils showed the highest antibacterial activity at 25% against Enterobacter agglomerans and Streptomyces sp., while only clove and oregano oils were effective against Bacillus sp. at all concentrations studied. This study has an important implication for the possible use of the natural products from plants in the control of biodeterioration of documentary heritage.Facultad de Ciencias Exacta

Environmentally friendly products of vegetal origin used in the control of microorganisms involved in biodeterioration of cultural heritage

Las sustancias químicas que tradicionalmente se han utilizado como antimicrobianas en la prevención del biodeterioro del patrimonio cultural, son tóxicas para el medioambiente y producen efectos negativos sobre la salud y el material. Desde la antigüedad, los extractos y los aceites esenciales de plantas “biocidas vegetales”, han sido utilizados como antimicrobianos en distintos campos. Son producidos por las plantas como mecanismos de defensa, poseen actividad de amplio espectro, son productos económicos y son biodegradables. El utilizar productos ambientalmente amigables se presenta como una solución viable, que muestra ventajas desde el punto de vista económico y medioambiental y resulta ser una salida ecológica que propicia la disminución del uso de sustancias químicas tóxicas y contaminantes para el medioambiente. El objetivo de este trabajo es presentar los estudios en los que se emplearon “biocidas vegetales” para el control de microorganismos participantes en los procesos de biodeterioro, que contaminan los ambientes de archivos y bibliotecas, y causan problemas a la salud humana. Los “biocidas vegetales” se obtuvieron de diversas plantas cosechadas de su hábitat natural en La Plata, Argentina y en La Habana, Cuba y se probaron frente a bacterias y hongos aislados de ambientes de archivos y bibliotecas y de materiales pertenecientes al patrimonio cultural. La actividad biocida se estudió empleando el método de difusión en agar. Los extractos y aceites fueron analizados por cromatografía en fase gaseosa acoplada a espectroscopia de masas (CG/EM). En la literatura se ha indicado que la actividad antimicrobiana de los “biocidas vegetales” es debida a sesquiterpenos, triterpenoides, flavonoides, esteroles, fenoles, alcaloides, ésteres, cumarinas, etc., algunos de ellos están presentes en los productos ensayados. La determinación de zonas de inhibición por el método de difusión en agar mostró diferencias en la efectividad.Chemicals that have traditionally been used as antimicrobial in preventing biodeterioration of cultural heritage are toxic to the environment and cause adverse health and material effects. Since ancient times, extracts and essential oils of plants "biocides plant" have been used as antimicrobials in different fields. They are produced by plants as defense mechanisms, have broad spectrum activity are economic products and are biodegradable. Using environmentally friendly products is presented as a viable solution shows advantages from the standpoint of economic and environmental and ecological output proves that encourages reduced use of toxic chemicals and pollutants to the environment. The aim of this paper is to present the studies that were used "biocides plant" for the control of microorganisms involved in biodeterioration processes and that pollute the environment of archives and libraries, and cause human health problems. The "biocides plants" were obtained from various plants harvested from natural habitat of La Plata, Argentina and Havana, Cuba, and were tested against bacteria and fungi isolated from archival and library environments and materials belonging to cultural heritage. The biocidal activity was studied using the agar diffusion method. Essential oils and extracts were analyzed by gas chromatography coupled to mass spectroscopy (GC/MS). The literature has indicated that the antimicrobial activity of "biocide plants" is due to sesquiterpenes, triterpenoids, flavonoids, sterol, phenols, alkaloids, esters, coumarins, etc., Some of them are present in the products tested. The determination of zones of inhibition by the agar diffusion method showed moderate effectiveness and / or positive in most plant biocides tested.Asociación de Universidades Grupo Montevide

Essential Oils of Plants as Biocides against Microorganisms Isolated from Cuban and Argentine Documentary Heritage

Natural products obtained from plants with biocidal activity represent an alternative and useful source in the control of biodeterioration of documentary heritage, without negative environmental and human impacts. In this work, we studied the antimicrobial activity of seven essential oils against microorganisms associated with the biodeterioration of documentary heritage. The essential oils were obtained by steam distillation. The antimicrobial activity was analyzed using the agar diffusion method against 4 strains of fungi and 6 bacterial strains isolated fromrepositories air and documents of theNational Archive of the Republic of Cuba and theHistorical Archive of theMuseum of La Plata, Argentina. Anise and garlic oils showed the best antifungal activity at all concentrations studied, while oregano oil not only was effective against fungi tested but also prevented sporulation of them all. Orange sweet and laurel oils were ineffective against fungi. Clove, garlic, and oregano oils showed the highest antibacterial activity at 25% against Enterobacter agglomerans and Streptomyces sp., while only clove and oregano oils were effective against Bacillus sp. at all concentrations studied. This study has an important implication for the possible use of the natural products from plants in the control of biodeterioration of documentary heritage.Facultad de Ciencias Exacta

Diagnóstico do estado de conservação do fundo Governo Superior Civil do Arquivo Nacional da República de Cuba

The study aims to conduct a comprehensive assessment of the “Gobierno Superior Civil” collection in order to determine deteriorations present, analyze the conditions of the vault where documents are kept, and evaluate temperature, relative humidity, and lighting conditions in the room. During 2013, the average values of temperature and relative humidity inside the vault were 29 °C and 59%, respectively. Illuminance values did not exceed those allowed for paper documents (50 lux), except when it came from artificial light on cloudy days. 312 bundles, from a total of 1677, were analyzed using the Diagnos program. Such bundles are composed almost entirely of rag paper written with iron gall ink. The predominant deteriorations were dirt, yellowing, brittle paper, with cracks and creases, and documents damaged due to oxidation of iron gall ink.El objetivo del estudio fue realizar el diagnóstico integral del fondo Gobierno Superior Civil para determinar los deterioros que presenta, analizar las condiciones del depósito donde se conserva y evaluar el comportamiento de la temperatura, la humedad relativa y la iluminancia en el local durante el 2013. En este año, los valores promedio de temperatura y humedad relativa en el interior del depósito fueron de 29 °C y 59 %, respectivamente. Los valores de iluminancia no sobrepasaron los permitidos para documentos en papel (50 lux) solo cuando proviene de la luz artificial en días nublados. De un total de 1677 legajos, se analizaron 312 empleando el programa Diagnos. Tales legajos están conformados casi en su totalidad por papel de trapo y fueron escritos con tinta ferrogálica. Los deterioros predominantes fueron suciedad, amarillamiento, papeles quebradizos, con roturas, con dobleces y documentos afectados por la oxidación de las tintas ferrogálicas.O objetivo do estudo foi realizar o diagnóstico integral do fundo Governo Superior Civil para determinar as deteriorações que se apresentam, para analisar as condições do depósito onde se conserva e avaliar o comportamento da temperatura, a umidade relativa e a iluminância no local durante o ano de 2013. Neste ano, os valores aproximados de temperatura e umidade relativa no interior do depósito foram de 29 °C e 59 %, respectivamente. Os valores de iluminância não ultrapassaram os permitidos para documentos em papel (50 lux) somente quando provém da luz artificial em dias nublados. De um total de 1677 ficheiros, se analisaram 312 empregando o programa Diagnos. Estes ficheiros estão conformados quase totalmente por papel de trapo e foram escritos com tinta ferrogálica. As deteriorações predominantes foram sujeira, amarelecimento, papel quebradiço, com roturas, com dobras e documentos afetados pela oxidação das tintas ferrogálicas