Introdução: Queer/Cuir das Américas: tradução, decolonialidade e o incomensurável

Editores: Diego Falconí Trávez(Universitat Autònoma de Barcelona/Universidad San Francisco de Quito),Lourdes Martínez-Echazábal (Universidade de Califórnia Santa Cruz/Universidade Federal deSanta Catarina), Joseph M. Pierce (Stony Brook University), Salvador Vidal-Ortiz (AmericanUniversity) e Maria Amelia Viteri (Universidad San Francisco de Quito)Editores: Diego Falconí Trávez(Universitat Autònoma de Barcelona/Universidad San Francisco de Quito),Lourdes Martínez-Echazábal (Universidade de Califórnia Santa Cruz/Universidade Federal deSanta Catarina), Joseph M. Pierce (Stony Brook University), Salvador Vidal-Ortiz (AmericanUniversity) e Maria Amelia Viteri (Universidad San Francisco de Quito)Editores: Diego Falconí Trávez(Universitat Autònoma de Barcelona/Universidad San Francisco de Quito),Lourdes Martínez-Echazábal (Universidade de Califórnia Santa Cruz/Universidade Federal deSanta Catarina), Joseph M. Pierce (Stony Brook University), Salvador Vidal-Ortiz (AmericanUniversity) e Maria Amelia Viteri (Universidad San Francisco de Quito

Comprehensive cross-platform comparison of methods for non-invasive EGFR mutation testing : results of the RING observational trial.

Abstract Several platforms for noninvasive EGFR testing are currently used in the clinical setting with sensitivities ranging from 30% to 100%. Prospective studies evaluating agreement and sources for discordant results remain lacking. Herein, seven methodologies including two next-generation sequencing (NGS)-based methods, three high-sensitivity PCR-based platforms, and two FDA-approved methods were compared using 72 plasma samples, from EGFR-mutant non-small-cell lung cancer (NSCLC) patients progressing on a first-line tyrosine kinase inhibitor (TKI). NGS platforms as well as high-sensitivity PCR-based methodologies showed excellent agreement for EGFR-sensitizing mutations (K = 0.80-0.89) and substantial agreement for T790M testing (K = 0.77 and 0.68, respectively). Mutant allele frequencies (MAFs) obtained by different quantitative methods showed an excellent reproducibility (intraclass correlation coefficients 0.86-0.98). Among other technical factors, discordant calls mostly occurred at mutant allele frequencies (MAFs) ≤ 0.5%. Agreement significantly improved when discarding samples with MAF ≤ 0.5%. EGFR mutations were detected at significantly lower MAFs in patients with brain metastases, suggesting that these patients risk for a false-positive result. Our results support the use of liquid biopsies for noninvasive EGFR testing and highlight the need to systematically report MAFs. Keywords: NGS; circulating free DNA; epidermal growth factor receptor; non-small-cell lung cancer; osimertinib; tyrosine kinase inhibitor

Seeking a relevant queerness: Sexual, racial and nationalist negotiations of identity amongst the Latino immigrant community in Washington, D.C.

Based on ethnographic work conducted between 2004 and 2006 with LGBT (Lesbian, Gay, Bisexual and Transgender) Latino community members living in the D.C. metro area, as well as summer research conducted in Ecuador and El Salvador during the summers of 2005 and 2006 respectively, I ethnographically map throughout this Anthropological research project how U.S. identity categories such as 'queer,' 'Latino,' 'American' are not stable categories but are constantly translated and as such reinvented and politicized according to diverse constructions of race and sexuality where notions of space are blurred with narratives from the 'homeland.' Having said this, I use 'queer' as an analytical tool to problematize the notion of a seamless relationship between identity and practice by illustrating the multiple unfixed meanings that 'Latino'-'American'- take as these categories are re-appropriated and translated by LGBT Latinos in D.C., El Salvador and Ecuador. I argue that LGBT 'Latinos and Latinas' negotiation towards and against a 'queer' and 'Latino' fixed identity act as a way to contest a 'western' (understood as colonial and Eurocentric) 'authority' embodied by these scripts and labels. This analysis is conducted considering the importance of understanding translation and border crossing as non-linear processes. The importance of looking at the intersection of categories such as 'Latino' and 'queer' that clash with discourses around citizenship particularly considering the current anti-immigration debate, produces new methodological and theoretical questions. These questions call to revisit the foundations of those same tools we use to conduct research where LGBT Latinos - and not (only) the theories about them - need to be exposed