6 research outputs found
Proteome and Allergenome of Asian Wasp, <i>Vespa affinis</i>, Venom and IgE Reactivity of the Venom Components
<i>Vespa affinis</i> (Asian
wasp, Thai banded tiger wasp,
or local name: Tor Hua Seua) causes the most frequent incidence of
medically important Hymenoptera sting in South and Southeast Asia.
However, data on the venom components attributable to the sting derived-clinical
manifestations (local reactions, IgE mediated-anaphylaxis, or systemic
envenomation) are lacking. This study provides the first set information
on <i>V. affinis</i> venom proteome, allergenome, and IgE
reactivity of individual venom components. From 2DE-gel based-proteomics,
the venom revealed 93 protein spots, of which proteins in 51 spots
could be identified and classified into three groups: typical venom
components and structural and housekeeping proteins. Venom proteins
in 32 spots reacted with serum IgE of wasp allergic patients. Major
allergenic proteins that reacted to IgE of >50% of the wasp allergic
patients included PLA<sub>1</sub> (100%), arginine kinase (73%), heat
shock 70 kDa protein (73.3%), venom allergen-5 (66.7%), enolase (66.7%),
PLA<sub>1</sub> magnifin (60%), glyceraldehyde-3-phosphate dehydrogenase
(60%), hyaluronidase (53.3%), and fructose-bisphosphate aldolase (53.3%).
The venom minor allergens were GB17876 transcript (40%), GB17291 transcript
(20%), malic enzyme (13.3%), aconitate hydratase (6.7%), and phosphoglucomutase
(6.7%). The information has diagnostic and clinical implications for
future improvement of case diagnostic sensitivity and specificity,
component-resolve diagnosis, and design of specific Hymenoptera venom
immunotherapy