35 research outputs found

    Retinoic acid regulates avian lung branching through a molecular network

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    Retinoic acid (RA) is of major importance during vertebrate embryonic development and its levels need to be strictly regulated otherwise congenital malformations will develop. Through the action of specific nuclear receptors, named RAR/RXR, RA regulates the expression of genes that eventually influence proliferation and tissue patterning. RA has been described as crucial for different stages of mammalian lung morphogenesis, and as part of a complex molecular network that contributes to precise organogenesis; nonetheless, nothing is known about its role in avian lung development. The current report characterizes, for the first time, the expression pattern of RA signaling members (stra6, raldh2, raldh3, cyp26a1, rar alpha, and rar beta) and potential RA downstream targets (sox2, sox9, meis1, meis2, tgf beta 2, and id2) by in situ hybridization. In the attempt of unveiling the role of RA in chick lung branching, in vitro lung explants were performed. Supplementation studies revealed that RA stimulates lung branching in a dose-dependent manner. Moreover, the expression levels of cyp26a1, sox2, sox9, rar beta, meis2, hoxb5, tgf beta 2, id2, fgf10, fgfr2, and shh were evaluated after RA treatment to disclose a putative molecular network underlying RA effect. In situ hybridization analysis showed that RA is able to alter cyp26a1, sox9, tgf beta 2, and id2 spatial distribution; to increase rar beta, meis2, and hoxb5 expression levels; and has a very modest effect on sox2, fgf10, fgfr2, and shh expression levels. Overall, these findings support a role for RA in the proximal-distal patterning and branching morphogenesis of the avian lung and reveal intricate molecular interactions that ultimately orchestrate branching morphogenesis.The authors would like to thank Ana Lima for slide sectioning and Rita Lopes for contributing to the initiation of this project. This work has been funded by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT), under the scope of the Project POCI-01-0145-FEDER-007038; and by the Project NORTE-01-0145- FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.info:eu-repo/semantics/publishedVersio

    Path dependence and the stabilization of strategic premises: how the funeral industry buries itself

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    POTENTIAL OF SILYBUM MARIANUM L. FOR PHYTOREMEDIATION OF SOILS CONTAMINATED WITH HEAVY METALS

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    Comparative research has been conducted to allow us to determine the accumulation of heavy metals, micro and macroelements in the vegetative and reproductive organs of milk thistle, the quality of milk thistle oil, as well as the possibilities to use the plant for phytoremediation of heavy metal contaminated soils. The experiment was performed on an agricultural field contaminated by the Non-Ferrous-Metal Works (NFMW) near Plovdiv, Bulgaria. The experimental plots were situated at different distances (0.5, and 15 km) from the source of pollution. The contents of heavy metals in plant materials (roots, stems, leaves, seeds and head seeds) were determined. The quality of milk thistle oils (heavy metals and fatty acid composition) was also determined. The quantitative measurements were carried out with inductively-coupled plasma (ICP). Mllk thistle is a plant that is tolerant to heavy metals and can be referred to the hyperaccumulators of lead and the accumulators of cadmium and zinc. The plant can be successfully used in the phytoremediation of heavy metal contaminated soils. Heavy metal soil contamination has no significant effect on the oil content of the milk thistle seeds and the fatty acid composition of the oil. Oil of milk thistle grown in contaminated soils is characterized by a lower content of saturated acids (with the exception of arachidonic acid) and polyunsaturated acids, and a higher monounsaturated acid content compared to the oil from the uncontaminated area. The processing of milk thistle seeds into oil and the use of the obtained oil will greatly reduce the cost of phytoremediation

    History, material memory and the temporality of identity construction

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    A growing body of research on how organizations engage with their histories has shown that organizational members revisit history in the light of present-day concerns to inspire or legitimate future courses of action. Studies of the processes through which organizational history is brought to bear on the present and future, however, remain rare. To uncover the processes and practices through which organizational members systematically engage with history, we investigated the uses of four corporate museums established by Italian manufacturers of consumer goods Alessi, Alfa Romeo, Ducati and Piaggio. We identified three distinct forms of engagement, reflecting different perspectives on the relationship between history and identity, involved different cognitive processes and emotional responses, and influenced organizational action in different ways. Our theoretical insights have significant implications not only for understanding the use of history in organizations, but also for research on organizational identity and organizational memory
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