The Importance of Support Networks for At-Promise Students

At-promise students enter colleges and universities with various challenges including being academically underprepared and lacking those essential critical thinking skills to be successful. However, providing support mechanisms within a nurturing environment can help these students overcome academic obstacles as well as personal challenges in order to achieve academic success. This article explores the role of an educational opportunity program and its efforts to support student success

METHOD FOR LIGHTWEIGHT REPRESENTATION AND COMPARISON OF CAD FILES

A method and system are proposed to greatly reduce the size of computer aided design (CAD) files. The system represents and stores the CAD files as a sequence of operations to create the end object. The sequence of operations describes the CAD geometry including a range of mathematical descriptions in text form. The CAD file is structured as a hierarchical set of operations. Each change in operation is stored as a delta version taking up very little storage space. Major versions are saved with graphics for quick view, similar to current CAD files storage. The textual representation of the CAD file is used for comparing and merging of parts/features if needed. The lightweight file size is easier for performing meaningful diff operations such as merging of different versions for collaboration and version management. Backups are made possible for every change allowing fine grained return to any previous state

Invisible design: exploring insights and ideas through ambiguous film scenarios

Invisible Design is a technique for generating insights and ideas with workshop participants in the early stages of concept development. It involves the creation of ambiguous films in which characters discuss a technology that is not directly shown. The technique builds on previous work in HCI on scenarios, persona, theatre, film and ambiguity. The Invisible Design approach is illustrated with three examples from unrelated projects; Biometric Daemon, Panini and Smart Money. The paper presents a qualitative analysis of data from a series of workshops where these Invisible Designs were discussed. The analysis outlines responses to the films in terms of; existing problems, concerns with imagined technologies and design speculation. It is argued that Invisible Design can help to create a space for critical and creative dialogue during participatory concept development

Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides

Phosphoinositides are a small family of phospholipids that act as signaling hubs and key regulators of cellular function. Detecting their subcellular distribution is crucial to gain insights into membrane organization and is commonly done by the overexpression of biosensors. However, this leads to cellular perturbations and is challenging in systems that cannot be transfected. Here, we present a toolkit for the reliable, fast, multiplex, and super-resolution detection of phosphoinositides in fixed cells and tissue, based on recombinant biosensors with self-labeling SNAP tags. These are highly specific and reliably visualize the subcellular distributions of phosphoinositides across scales, from 2D or 3D cell culture to Drosophila tissue. Further, these probes enable super-resolution approaches, and using STED microscopy, we reveal the nanoscale organization of PI(3)P on endosomes and PI(4)P on the Golgi. Finally, multiplex staining reveals an unexpected presence of PI(3,5)P2-positive membranes in swollen lysosomes following PIKfyve inhibition. This approach enables the versatile, high-resolution visualization of multiple phosphoinositide species in an unprecedented manner.</p

Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides

Phosphoinositides are a small family of phospholipids that act as signaling hubs and key regulators of cellular function. Detecting their subcellular distribution is crucial to gain insights into membrane organization and is commonly done by the overexpression of biosensors. However, this leads to cellular perturbations and is challenging in systems that cannot be transfected. Here, we present a toolkit for the reliable, fast, multiplex, and super-resolution detection of phosphoinositides in fixed cells and tissue, based on recombinant biosensors with self-labeling SNAP tags. These are highly specific and reliably visualize the subcellular distributions of phosphoinositides across scales, from 2D or 3D cell culture to Drosophila tissue. Further, these probes enable super-resolution approaches, and using STED microscopy, we reveal the nanoscale organization of PI(3)P on endosomes and PI(4)P on the Golgi. Finally, multiplex staining reveals an unexpected presence of PI(3,5)P2-positive membranes in swollen lysosomes following PIKfyve inhibition. This approach enables the versatile, high-resolution visualization of multiple phosphoinositide species in an unprecedented manner.</p

Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides

Phosphoinositides are a small family of phospholipids that act as signaling hubs and key regulators of cellular function. Detecting their subcellular distribution is crucial to gain insights into membrane organization and is commonly done by the overexpression of biosensors. However, this leads to cellular perturbations and is challenging in systems that cannot be transfected. Here, we present a toolkit for the reliable, fast, multiplex, and super-resolution detection of phosphoinositides in fixed cells and tissue, based on recombinant biosensors with self-labeling SNAP tags. These are highly specific and reliably visualize the subcellular distributions of phosphoinositides across scales, from 2D or 3D cell culture to Drosophila tissue. Further, these probes enable super-resolution approaches, and using STED microscopy, we reveal the nanoscale organization of PI(3)P on endosomes and PI(4)P on the Golgi. Finally, multiplex staining reveals an unexpected presence of PI(3,5)P2-positive membranes in swollen lysosomes following PIKfyve inhibition. This approach enables the versatile, high-resolution visualization of multiple phosphoinositide species in an unprecedented manner

Health Related Quality of Life and Return to Work After Minor Extremity Injuries: a Longitudinal Study Comparing Upper Versus Lower Extremity Injuries

Purpose: To investigate the impact on health related quality of life (HRQL) during the first year after minor extremity injury and to determine whether there is a difference in recovery patterns and return to work between upper extremity injuries (UEI) and lower extremity injuries (LEI). Method: A total of 181 adults’ age 18 years or older randomly selected from patients admitted to an emergency department with minor injuries were studied. HRQL was measured using the Functional Status Questionnaire (FSQ) at 1–2 weeks, 3, 6, and 12-months post-injury. Pre-injury FSQ scores were measured retrospectively at admission. A quasi-least square (QLS) model was constructed to examine differences of FSQ scores at each measuring point for UEI and LEI. Results: Fractures of the knee/lower leg (25%) were the most frequently injured body area. Slips or falls (57%) and traffic-related events (22%) were the most common injury causes. The mean ISS was 4.2 (SD 0.86). Both groups had significant declines in the FSQ scores physical and social functioning at 1–2 weeks after injury. Patients with UEI made larger improvements in the first 3 months post-injury versus patients with LEI whose improvements extended over the first 6 months. None of the groups reached the pre-injury FSQ scores during the first post-injury year except in the subscale work performance where UEI exceeded the pre-injury scores. At 12 months post-injury, significant lower FSQ scores remained in the LEI group compared to the UEI group in intermediate activities of daily living (p = 0.036, d 0.4) and work performance (p = 0.004, d 0.7). The return to work at 3 months and 12 months were 76% and 88% for UEI and 58% and 77% for LEI. No significant differences were found between groups in the FSQ scale mental health and social interaction. Conclusions: LEI had the highest impact on HRQL and return to work during the first year which exceeded the consequences of UEI. These findings contribute to the information about the consequences of injury in order to give sufficient prognostic information to patients and different stakeholders. Future investigations should aim to investigate specific minor extremity injuries and identify factors that facilitate recovery and return to work

GJ357: A low-mass planetary system uncovered by precision radial-velocities and dynamical simulations

We report the detection of a new planetary system orbiting the nearby M2.5V star GJ357, using precision radial-velocities from three separate echelle spectrographs, HARPS, HiRES, and UVES. Three small planets have been confirmed in the system, with periods of 9.125+/-0.001, 3.9306+/-0.0003, and 55.70+/-0.05 days, and minimum masses of 3.33+/-0.48, 2.09+/-0.32, and 6.72+/-0.94 Me, respectively. The second planet in our system, GJ357c, was recently shown to transit by the Transiting Exoplanet Survey Satellite (TESS; Luque et al. 2019), but we could find no transit signatures for the other two planets. Dynamical analysis reveals the system is likely to be close to coplanar, is stable on Myrs timescales, and places strong upper limits on the masses of the two non-transiting planets b and d of 4.25 and 11.20 Me, respectively. Therefore, we confirm the system contains at least two super-Earths, and either a third super-Earth or mini-Neptune planet. GJ357b & c are found to be close to a 7:3 mean motion resonance, however no libration of the orbital parameters was found in our simulations. Analysis of the photometric lightcurve of the star from the TESS, when combined with our radial-velocities, reveal GJ357c has an absolute mass, radius, and density of 2.248+0.117-0.120 Me, 1.167+0.037-0.036 Re, and 7.757+0.889-0.789 g/cm3, respectively. Comparison to super-Earth structure models reveals the planet is likely an iron dominated world. The GJ357 system adds to the small sample of low-mass planetary systems with well constrained masses, and further observational and dynamical follow-up is warranted to better understand the overall population of small multi-planet systems in the solar neighbourhood.Comment: Manuscript accepted to MNRAS 15/10/201