The initial shear field in models with primordial local non-Gaussianity and implications for halo and void abundances

We generalize the Doroshkevich's celebrated formulae for the eigenvalues of the initial shear field associated with Gaussian statistics to the local non-Gaussian fnl model. This is possible because, to at least second order in fnl, distributions at fixed overdensity are unchanged from the case fnl= 0. We use this generalization to estimate the effect of fnl≠ 0 on the abundance of virialized haloes. Halo abundances are expected to be related to the probability that a certain quantity in the initial fluctuation field exceeds a threshold value, and we study two choices for this variable: it can either be the sum of the eigenvalues of the initial deformation tensor (the initial overdensity) or its smallest eigenvalue. The approach based on a critical overdensity yields results which are in excellent agreement with numerical measurements. We then use these same methods to develop approximations describing the sensitivity of void abundances on fnl. While a positive fnl produces more extremely massive haloes, it makes fewer extremely large voids. Its effect thus is qualitatively different from a simple rescaling of the normalization of the density fluctuation field σ8. Therefore, void abundances furnish complementary information to cluster abundances, and a joint comparison of both might provide interesting constraints on primordial non-Gaussianit

The non-linear redshift space probability distribution function in models with local primordial non-Gaussianity

We use the ellipsoidal collapse approximation to investigate the non-linear redshift space evolution of the density field with primordial non-Gaussianity of the local fnl-type. We utilize the joint distribution of eigenvalues of the initial non-Gaussian shear field and evaluate the evolved redshift space probability distribution function (PDF). It is shown that, similar to the real space analysis, the underdense tail of the non-linear redshift space PDF differs significantly from that for Gaussian initial conditions. We also derive the lowest order correction of the Kaiser's formula in the presence of a non-zero fn

The nonlinear redshift space probability distribution function in models with local primordial non-Gaussianity

We use the ellipsoidal collapse approximation to investigate the nonlinear redshift space evolution of the density field with primordial non-Gaussianity of the local f_{nl}-type. We utilize the joint distribution of eigenvalues of the initial non-Gaussian shear field and evaluate the evolved redshift space probability distribution function (PDF). It is shown that, similar to the real space analysis, the underdense tail of the nonlinear redshift space PDF differs significantly from that for Gaussian initial conditions. We also derive the lowest order correction of the Kaiser's formulain the presence of a non-zero f_{nl}.Comment: Matched version accepted by MNRA

Lack of PD-L1 Expression by iNKT Cells Improves the Course of Influenza A Infection

There is evidence indicating that invariant Natural Killer T (iNKT) cells play an important role in defense against influenza A virus (IAV). However, the effect of inhibitory receptor, programmed death-1 (PD-1), and its ligands, programmed death ligand (PD-L) 1 and 2 on iNKT cells in protection against IAV remains to be elucidated. Here we investigated the effects of these co-stimulatory molecules on iNKT cells in the response to influenza. We discovered that compare to the wild type, PD-L1 deficient mice show reduced sensitivity to IAV infection as evident by reduced weight loss, decreased pulmonary inflammation and cellular infiltration. In contrast, PD-L2 deficient mice showed augmented weight loss, pulmonary inflammation and cellular infiltration compare to the wild type mice after influenza infection. Adoptive transfer of iNKT cells from wild type, PD-L1 or PD-L2 deficient mice into iNKT cell deficient mice recapitulated these findings. Interestingly, in our transfer system PD-L1−/−-derived iNKT cells produced high levels of interferon-gamma whereas PD-L2−/−-derived iNKT cells produced high amounts of interleukin-4 and 13 suggesting a role for these cytokines in sensitivity to influenza. We identified that PD-L1 negatively regulates the frequency of iNKT cell subsets in the lungs of IAV infected mice. Altogether, these results demonstrate that lack of PD-L1 expression by iNKT cells reduces the sensitivity to IAV and that the presence of PD-L2 is important for dampening the deleterious inflammatory responses after IAV infection. Our findings potentially have clinical implications for developing new therapies for influenza

Efficacy of using cancer stem cell markers in isolating and characterizing liver cancer stem cells

Recent evidence suggests that a subset of hepatocellular carcinomas (HCCs) are derived from liver cancer stem cells (LCSCs). In order to isolate and characterize LCSCs, reliable markers that are specific to these cells are required. We evaluated the efficacy of a range of cancer stem cell (CSC) markers in isolating and characterizing LCSCs. We show that the most widely used CSC markers are not specific to LCSCs. By western analysis, protein expression of the common markers showed no significant difference between HCC tumor tissues and adjacent non-cancerous liver. Further, isolation of LCSCs from common HCC cell lines using FACScan and microbeads showed no consistent marker expression pattern. We also show that LCSCs have unique subtypes. Immunohistochemistry of HCC tissues showed that different HCCs express unique combinations of LCSC markers. Quantitative real-time polymerase chain reaction analysis showed that LCSCs isolated using different markers in the same HCC phenotype had different expression profiles. Likewise, LCSCs isolated from different HCC phenotypes with the same marker also had unique expression profiles and displayed varying resistance profiles to Sorafenib. Thus, using a range of commonly used CSC markers in HCCs and cell lines, we demonstrate that currently available markers are not specific for LCSCs. LCSCs have unique subtypes that express distinctive combinations of LCSC markers and altered drug resistance profiles, making their identification problematic

The initial shear field in models with primordial local non-Gaussianity and implications for halo and void abundances

We generalize Doroshkevich's celebrated formulae for the eigenvalues of the initial shear field associated with Gaussian statistics to the local non-Gaussian f_{nl} model. This is possible because, to at least second order in f_{nl}, distributions at fixed overdensity are unchanged from the case f_{nl}=0. We use this generalization to estimate the effect of f_{nl}\ne 0 on the abundance of virialized halos. Halo abundances are expected to be related to the probability that a certain quantity in the initial fluctuation field exceeds a threshold value, and we study two choices for this variable: it can either be the sum of the eigenvalues of the initial deformation tensor (the initial overdensity), or its smallest eigenvalue. The approach based on a critical overdensity yields results which are in excellent agreement with numerical measurements. We then use these same methods to develop approximations describing the sensitivity of void abundances on f_{nl}. While a positive f_{nl} produces more extremely massive halos, it makes fewer extremely large voids. Its effect thus is qualitatively different from a simple rescaling of the normalisation of the density fluctuation field \sigma_8. Therefore, void abundances furnish complementary information to cluster abundances, and a joint comparison of both might provide interesting constraints on primordial non-Gaussianity.Comment: 16 pages, 8 figures, match version accepted by MNRA

Surveillance of emerging drugs of abuse in Hong Kong: Validation of an analytical tool

© 2015, Hong Kong Academy of Medicine Press. All rights reserved. Objective: To validate a locally developed chromatography-based method to monitor emerging drugs of abuse whilst performing regular drug testing in abusers. Design: Cross-sectional study. Setting: Eleven regional hospitals, seven social service units, and a tertiary level clinical toxicology laboratory in Hong Kong. Participants: A total of 972 drug abusers and high-risk individuals were recruited from acute, rehabilitation, and high-risk settings between 1 November 2011 and 31 July 2013. A subset of the participants was of South Asian ethnicity. In total, 2000 urine or hair specimens were collected. Main outcome measures: Proof of concept that surveillance of emerging drugs of abuse can be performed whilst conducting routine drug of abuse testing in patients. Results: The method was successfully applied to 2000 samples with three emerging drugs of abuse detected in five samples: PMMA (paramethoxymethamphetamine), TFMPP [1-(3-trifluoromethylphenyl)piperazine], and methcathinone. The method also detected conventional drugs of abuse, with codeine, methadone, heroin, methamphetamine, and ketamine being the most frequently detected drugs. Other findings included the observation that South Asians had significantly higher rates of using opiates such as heroin, methadone, and codeine; and that ketamine and cocaine had significantly higher detection rates in acute subjects compared with the rehabilitation population. Conclusions: This locally developed analytical method is a valid tool for simultaneous surveillance of emerging drugs of abuse and routine drug monitoring of patients at minimal additional cost and effort. Continued, proactive surveillance and early identification of emerging drugs will facilitate prompt clinical, social, and legislative management.Link_to_subscribed_fulltex

HD 191939 revisited: New and refined planet mass determinations, and a new planet in the habitable zone

HD 191939 (TOI-1339) is a nearby (d = 54 pc), bright (V = 9 mag), and inactive Sun-like star (G9 V) known to host a multi-planet transiting system. Ground-based spectroscopic observations confirmed the planetary nature of the three transiting sub-Neptunes (HD 191939 b, c, and d) originally detected by TESS and were used to measure the masses for planets b and c with 3\ucf precision. These previous observations also reported the discovery of an additional Saturn-mass planet (HD 191939 e) and evidence for a further, very long-period companion (HD 191939 f). Here, we report the discovery of a new non-transiting planet in the system and a refined mass determination of HD 191939 d. The new planet, HD 191939 g, has a minimum mass of 13.5\ub12.0 M- and a period of about 280 days. This period places the planet within the conservative habitable zone of the host star, and near a 1:3 resonance with HD 191939 e. The compilation of 362 radial velocity measurements with a baseline of 677 days from four different high-resolution spectrographs also allowed us to refine the properties of the previously known planets, including a 4.6\ucf mass determination for planet d, for which only a 2\ucf upper limit had been set until now. We confirm the previously suspected low density of HD 191939 d, which makes it an attractive target for attempting atmospheric characterisation. Overall, the planetary system consists of three sub-Neptunes interior to a Saturn-mass and a Uranus-mass planet plus a high-mass long-period companion. This particular configuration has no counterpart in the literature and makes HD 191939 an exceptional multi-planet transiting system with an unusual planet demographic worthy of future observation