Effect of front and back squat techniques on peak loads experienced by the Achilles tendon

Background A primary technique in the discipline of strength and conditioning the squat has two principal ‘back and front’ variants. Despite the physiological and strength benefits of the squat, the propensity for musculoskeletal injury is high. The current investigation examined the influence of the front and back squat variations on the load experienced by the Achilles tendon. Material/Methods Achilles tendon loads were obtained from eighteen experienced male participants as they completed both back and front squats. Differences between squat conditions were examined using Bonferroni adjusted (p = 0.0125) paired t-tests. Results The results showed that the peak Achilles tendon load was significantly greater in the back squat (2.67 ±0.74 B.W) condition compared to the front squat (2.37 ±0.69 B.W). Conclusions Given the proposed relationship between the magnitude of the load experienced by the Achilles tendon and tendon pathology, the back squat appears to place lifters at greater risk from Achilles tendon injury. Therefore, it may be prudent for lifters who are predisposed to Achilles tendon pathology to utilize the front squat in their training

Effects of foot orthoses on patellofemoral load in recreational runners

The most common chronic injury in recreational runners is patellofemoral pain. Whilst there is evidence to suggest that orthotic intervention may reduce symptoms in runners who experience patellofemoral pain the mechanism by which their clinical effects are mediated is currently poorly understood. The aim of the current investigation was to determine whether foot orthoses reduce the loads experienced by the patellofemoral joint during running. Patellofemoral loads were obtained from fifteen male runners who ran at 4.0 m·s-1. Patellofemoral loads with and without orthotics were contrasted using paired t-tests. The results showed that patellofemoral joint loads were significantly reduced as a function of running with the orthotic device. The current investigation indicates that through reductions in patellofemoral loads, foot orthoses may serve to reduce the incidence of chronic running injuries at this joint

Modelling Muscle Force Distributions during the Front and Back Squat in Trained Lifters

The barbell squat is a fundamental strength and conditioning exercise, with two principal variants; back and front. Whilst previous studies have examined the mechanical differences of the front and back squat, there is no information comparing the distributions of muscle forces between these variants. This study aimed to compare estimated forces developed by the primary skeletal muscles used in the front and back squat. Twenty-five male participants were recruited with 6.24 ±2.21 years of experience in squat lifting and 1 repetition maximum values of 127.5 ±18.8 and 90.6 ±14.4 kg for the back and front squat lifts. Participants completed both back and front squats at 70% of their front squat 1 repetition maximum. Muscle forces were determined during dynamic situations using motion capture data, in addition to sagittal plane kinematics. Differences between squat conditions were examined using a multivariate analysis of variance. The kinematic analysis showed that the back squat was associated with significantly (p 0.05). Our results indicate that neither the front nor back squat provides any marked difference in muscle force production, aside from that isolated to the lower back. These findings lead the conclusion that neither the front nor back squat conditions confer any additional benefits over the other in terms of the skeletal muscle force output

Effects of front and back squat techniques on patellofemoral joint kinetics in males

Purpose: The barbell squat is fundamental in strength and conditioning, with two principal variants; the back and front squat. Unfortunately, the propensity for injury is high particularly at the knee. The aim of the current investigation was examine the influence of front and back squat variations on patellofemoral joint load. Methods: Patellofemoral loads were obtained from thirty-five experienced male participants, who completed both back and front squats at 70% of 1 RM. Differences between squat conditions were examined using Bonferroni adjusted (P = 0.008) paired t-tests. Results: The results showed that significant differences (P < .008) in patellofemoral load were identified between both conditions with the highest load being experienced during the back squat exercise. Conclusions: Given the proposed relationship between the magnitude of the load experienced by the patellofemoral joint and associated injury pathology, the back squat appears to place lifters at greater risk from injury. Therefore, it may be prudent therefore for lifters who are predisposed to patellofemoral pain syndrome to utilize the front squat in their training

Effects of Varus Orthotics on Lower Extremity Kinematics During the Pedal Cycle

Purpose. Cycling has been shown to be associated with a high incidence of chronic pathologies. Foot orthoses are frequently used by cyclists in order to reduce the incidence of chronic injuries. The aim of the current investigation was to examine the influence of different varus orthotic inclines on the three-dimensional kinematics of the lower extremities during the pedal cycle. Methods. Kinematic information was obtained from ten male cyclists using an eight-camera optoelectronic 3-D motion capture system operating at 250 Hz. Participants cycled with and without orthotic intervention at three different cadences (70, 90 and 110 RPM). The orthotic device was adjustable and four different wedge conditions (0 mm – no orthotic, 1.5 mm, 3.0 mm and 4.5 mm) were examined. Two-way repeated measures ANOVAs were used to compare the kinematic parameters obtained as a function of orthotic inclination and cadence. Participants were also asked to subjectively rate their comfort in cycling using each of the four orthotic devices on a 10-point Likert scale. Results. The kinematic analysis indicated that the orthotic device had no significant influence at any of the three cadences. Analysis of subjective preferences showed a clear preference for the 0 mm, no orthotic, condition. Conclusions. This study suggests that foot orthoses do not provide any protection from skeletal malalignment issues associated with the aetiology of chronic cycling injuries

TWEAK and Fn14 expression in the pathogenesis of joint inflammation and bone erosion in rheumatoid arthritis

Extent: 10p.INTRODUCTION: TNF-like weak inducer of apoptosis (TWEAK) has been proposed as a mediator of inflammation and bone erosion in rheumatoid arthritis (RA). This study aimed to investigate TWEAK and TWEAK receptor (Fn14) expression in synovial tissue from patients with active and inactive rheumatoid arthritis (RA), osteoarthritis (OA) and normal controls and assess soluble (s)TWEAK levels in the synovial fluids from patients with active RA and OA. Effects of sTWEAK on osteoclasts and osteoblasts were investigated in vitro. METHODS: TWEAK and Fn14 expression were detected in synovial tissues by immunohistochemistry (IHC). Selected tissues were dual labelled with antibodies specific for TWEAK and lineage-selective cell surface markers CD68, Tryptase G, CD22 and CD38. TWEAK mRNA expression was examined in human peripheral blood mononuclear cells (PBMC) sorted on the basis of their expression of CD22. sTWEAK was detected in synovial fluid from OA and RA patients by ELISA. The effect of sTWEAK on PBMC and RAW 264.7 osteoclastogenesis was examined. The effect of sTWEAK on cell surface receptor activator of NF Kappa B Ligand (RANKL) expression by human osteoblasts was determined by flow cytometry. RESULTS: TWEAK and Fn14 expression were significantly higher in synovial tissue from all patient groups compared to the synovial tissue from control subjects (P < 0.05). TWEAK was significantly higher in active compared with inactive RA tissues (P < 0.05). TWEAK expression co-localised with a subset of CD38+ plasma cells and with CD22+ B-lymphocytes in RA tissues. Abundant TWEAK mRNA expression was detected in normal human CD22+ B cells. Higher levels of sTWEAK were observed in synovial fluids isolated from active RA compared with OA patients. sTWEAK did not stimulate osteoclast formation directly from PBMC, however, sTWEAK induced the surface expression of RANKL by human immature, STRO-1+ osteoblasts. CONCLUSIONS: The expression of TWEAK by CD22+ B cells and CD38+ plasma cells in RA synovium represents a novel potential pathogenic pathway. High levels of sTWEAK in active RA synovial fluid and of TWEAK and Fn14 in active RA tissue, together with the effect of TWEAK to induce osteoblastic RANKL expression, is consistent with TWEAK/Fn14 signalling being important in the pathogenesis of inflammation and bone erosion in RA.Anak A. S. S. K. Dharmapatni, Malcolm D. Smith, Tania N. Crotti, Christopher A. Holding, Cristina Vincent, Helen M. Weedon, Andrew C. W. Zannettino, Timothy S. Zheng, David M. Findlay, Gerald J. Atkins and David R. Hayne

An Investigation into the Potential of Targeting Escherichia coli rne mRNA with Locked Nucleic Acid (LNA) Gapmers as an Antibacterial Strategy

The increase in antibacterial resistance is a serious challenge for both the health and defence sectors and there is a need for both novel antibacterial targets and antibacterial strategies. RNA degradation and ribonucleases, such as the essential endoribonuclease RNase E, encoded by the rne gene, are emerging as potential antibacterial targets while antisense oligonucleotides may provide alternative antibacterial strategies. As rne mRNA has not been previously targeted using an antisense approach, we decided to explore using antisense oligonucleotides to target the translation initiation region of the Escherichia coli rne mRNA. Antisense oligonucleotides were rationally designed and were synthesised as locked nucleic acid (LNA) gapmers to enable inhibition of rne mRNA translation through two mechanisms. Either LNA gapmer binding could sterically block translation and/or LNA gapmer binding could facilitate RNase H-mediated cleavage of the rne mRNA. This may prove to be an advantage over the majority of previous antibacterial antisense oligonucleotide approaches which used oligonucleotide chemistries that restrict the mode-of-action of the antisense oligonucleotide to steric blocking of translation. Using an electrophoretic mobility shift assay, we demonstrate that the LNA gapmers bind to the translation initiation region of E. coli rne mRNA. We then use a cell-free transcription translation reporter assay to show that this binding is capable of inhibiting translation. Finally, in an in vitro RNase H cleavage assay, the LNA gapmers facilitate RNase H-mediated mRNA cleavage. Although the challenges of antisense oligonucleotide delivery remain to be addressed, overall, this work lays the foundations for the development of a novel antibacterial strategy targeting rne mRNA with antisense oligonucleotides

Resolving the apparent transmission paradox of African sleeping sickness

Human African trypanosomiasis (HAT), or African sleeping sickness, is a fatal disease found throughout sub-Saharan Africa. The disease is close to elimination in many areas, although it was similarly close to elimination once before and subsequently reemerged, despite seemingly low rates of transmission. Determining how these foci persisted and overcame an apparent transmission paradox is key to finally eliminating HAT. By assessing clinical, laboratory, and mathematical data, we propose that asymptomatic infections contribute to transmission through the presence of an overlooked reservoir of skin-dwelling parasites. Our assessment suggests that a combination of asymptomatic and parasitaemic cases is sufficient to maintain transmission at foci without animal reservoirs, and we argue that the current policy not to treat asymptomatic HAT should be reconsidered