Patterns of antenal sensilla of Panstrongylus megistus from three Brazilian states

The objective of the present study was to analyze and describe the phenotype of the antennal sensilla of Panstrongylus megistus, one of the epidemiologically most important species of triatomines in Brazil. Specimens from the Brazilian states of Goiás (GO), Minas Gerais (MG), and Rio Grande do Sul (RS) were compared, based on studies of four types of sensilla on three antennal segments: thick-walled trichoid (TK), thin-walled trichoid (TH), bristles (BR), and basiconica (BA). Discriminant analysis allowed the separation of the RS specimens from those of GO and MG. Multivariate discriminant analysis demonstrated that the sensilla of males differed from those of females, the variables with greatest weight being the BA of all three segments and the TK of flagellum 1. The basiconica sensilla were significantly more abundant in females, on all three segments. Antennal sensilla patterns also demonstrated significant differences among P. megistus specimens.Fil: Villela, M. M.. Fundación Oswaldo Cruz; BrasilFil: Catala, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de Catamarca. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Secretaría de Industria y Minería. Servicio Geológico Minero Argentino. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Provincia de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; ArgentinaFil: Juberg, J.. Instituto Oswaldo Cruz; Brasil. Fundación Oswaldo Cruz; BrasilFil: Silva, I. G.. Universidade Federal de Goiás; BrasilFil: Dias, J. C. P.. Fundación Oswaldo Cruz; Brasi

Reprogramming of lysosomal gene expression by interleukin-4 and Stat6.

BACKGROUND: Lysosomes play important roles in multiple aspects of physiology, but the problem of how the transcription of lysosomal genes is coordinated remains incompletely understood. The goal of this study was to illuminate the physiological contexts in which lysosomal genes are coordinately regulated and to identify transcription factors involved in this control. RESULTS: As transcription factors and their target genes are often co-regulated, we performed meta-analyses of array-based expression data to identify regulators whose mRNA profiles are highly correlated with those of a core set of lysosomal genes. Among the ~50 transcription factors that rank highest by this measure, 65% are involved in differentiation or development, and 22% have been implicated in interferon signaling. The most strongly correlated candidate was Stat6, a factor commonly activated by interleukin-4 (IL-4) or IL-13. Publicly available chromatin immunoprecipitation (ChIP) data from alternatively activated mouse macrophages show that lysosomal genes are overrepresented among Stat6-bound targets. Quantification of RNA from wild-type and Stat6-deficient cells indicates that Stat6 promotes the expression of over 100 lysosomal genes, including hydrolases, subunits of the vacuolar H⁺ ATPase and trafficking factors. While IL-4 inhibits and activates different sets of lysosomal genes, Stat6 mediates only the activating effects of IL-4, by promoting increased expression and by neutralizing undefined inhibitory signals induced by IL-4. CONCLUSIONS: The current data establish Stat6 as a broadly acting regulator of lysosomal gene expression in mouse macrophages. Other regulators whose expression correlates with lysosomal genes suggest that lysosome function is frequently re-programmed during differentiation, development and interferon signaling

Fast approaches for Bayesian estimation of size of hard-to-reach populations using Network Scale-up

The Network scale-up method is commonly used to overcome difficulties in estimating the size of hard-to-reach populations. The method uses indirect information based on social network of each participant taken from the general population, but in some applications a fast computational approach would be highly recommended. We propose a Gibbs sampling method and a Monte Carlo approach to sample from the random degree model. We applied the abovementioned analytical strategies to previous data on heavy drug users from Curitiba, Brazil

SIGMA Observations of the Bursting Pulsar GRO J1744-28

We present the results of the GRANAT/SIGMA hard X-/soft gamma-ray long-term monitoring of the Galactic Center (GC) region concerning the source GRO J1744-28, discovered on 1995 Dec. 2 by CGRO/BATSE. SIGMA observed the region containing the source in 14 opportunities between 1990 and 1997. In two of these observing sessions, corresponding to March 1996 and March 1997, GRO J1744-28 was detected with a confidence level greater than 5(sigma) in the 35-75 keV energy band without detection in the 75-150 keV energy band. For the other sessions, upper limits of the flux are indicated. The particular imaging capabilities of the SIGMA telescope allow us to identify, specifically, the source position in the very crowded GC region, giving us a mean flux of (73.1 +/- 5.5)E-11 and (44.7 +/- 6.4)E-11 ergs cm^-2 s^-1 in the 35-75 keV energy band, for the March 1996 and March 1997 observing sessions, respectively. Combining the March 1997 SIGMA and BATSE observations, we found evidence pointing to the type-II nature of the source bursts for this period. For the same observing campaigns, spectra were obtained in the 35 to 150 keV energy band. The best fit corresponds to an optically thin thermal Bremsstrahlung with F(50 keV)=(3.6 +/- 0.6)E-4 phot cm^-2 s^-1 keV^-1 and kT(Bremss)=28 +/- 7 keV, for the first campaign, and F(50 keV)=(2.3 +/- 0.7)E-4 phot cm^-2 s^-1 keV^-1 and kT(Bremss)=18 (+12/-7) keV, for the second. This kind of soft spectrum is typical of binary sources containing a neutron star as the compact object, in contrast to the harder spectra typical of systems containing a black hole candidateComment: Accepted for publication in the Astrophysical Journal, 4 pages, 4 figure