Monitoring Activity Of Living Cells Marked With Colloidal Semiconductor Quantum Dots

Fluorescent semiconductor nanocrystals in quantum confinement regime (quantum dots) present several well known features which make them very useful tools for biological labeling purposes. Low photo-bleaching rates, high chemical stability, active surface allowing conjugation to living cells, explains the success of this labeling procedure over the commonly used fluorescent dyes. In this paper we report the results obtained with high fluorescent core-shell CdTe-CdS (diameter = 3-7 nm) colloidal nanocrystals synthesized in aqueous medium and conjugated to glucose molecules, incubated with living yeast cells, in order to investigate their glucose up-take activity.6088Menezes, F.D., Brasil Jr., A.G., Moreira, W.L., Barbosa, L.C., Ferreira, R.C., Cerar, C.L., Farias, P.M.A., Santos, B.S., (2005) Microelectronics Journal, 36, pp. 989-991Alivisatos, A., (1996) J. Phys. Chem., 100, p. 13226Castro, V., Farias, P.M.A., Santos, B.S., Menezes, F.D., Ferreira, R., Fontes, A., Lima, P.R.M., Cesar, C.L., (2004) Blood, 104 (11), pp. 741aBrus, L.E., (1991) Appl. Phys. A, 53, p. 465Weller, H., (1993) Adv. Mater., 5 (2), p. 88Brus, L.E., Harkless, J.A.W., Stillinger, F.H., (1996) J. Am. Chem. Soc., 118, p. 4834Schaller, R.D., Klimov, V.I., (2004) Phys. Rev. Lett., 92, p. 186601Gao, M., Sun, J., Dulkeith, E., Gaponik, N., Lemmer, U., Feldmann, J., (2002) Langmuir, 18, p. 4098Pileni, M.P., (2000) Catal. Today, 58, p. 151Moreira, W.L., Fontes, A., Neves, A.A.R., Thomas, A.A., Barbosa, L.C., De Menezes, F.D., Farias, P.M.A., Cesar, C.L., (2005) Proc. Spie: Genet. Eng. Opt. Probes Biomed. Appl. III, 5704, p. 36Farias, P.M.A., Santos, B.S., Menezes, F.D., Cesar, C.L., Fontes, A., Barjas-Castro, M.L., Castro, V., Ferreira, R.C., (2005) Journal of Biomedical Optics, 10 (4), pp. 44023-44026Farias, P.M.A., Santos, B.S., Menezes, F.D., Cesar, C.L., Fontes, A., Barjas-Castro, M.L., Castro, V., Ferreira, R.C., (2005) Journal of Microscopy, 219 (3), pp. 103-108Borchert, H., Talapin, D.V., Gaponik, N., McGinley, C., Adam, S., Lobo, A., Möller, T., Weller, H., (2003) J. Phys. Chem. B, 107, p. 9662Rockenberger, J., Trögr, L., Rogach, A.L., Tischer, M., Grundmann, M., Eychmüller, A., Weller, H., (1998) J. Chem. Phys, 108, p. 780